In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens
"nBackground: Cockroaches have been recognized as a powerful indoor allergen. Cockroach allergy can be a major factor in seri­ous asthma and nasal allergy. Bioinformatics tools have been developed to identify potential allergens. The present study was conducted to identify poten...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2010-09-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/16702.pdf&manuscript_id=16702 |
| _version_ | 1818675345900961792 |
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| author | A Ahmed K Minhas Namood-e-Sahar O Aftab F Sher Khan |
| author_facet | A Ahmed K Minhas Namood-e-Sahar O Aftab F Sher Khan |
| author_sort | A Ahmed |
| collection | DOAJ |
| description | "nBackground: Cockroaches have been recognized as a powerful indoor allergen. Cockroach allergy can be a major factor in seri­ous asthma and nasal allergy. Bioinformatics tools have been developed to identify potential allergens. The present study was conducted to identify potential allergens in Periplaneta americana (Linnaeus)."nMethods: The study focused on the identification of potential allergens among the characterized proteins of P. americana us­ing web-based and publicly available allergen prediction tools that follow the FAO/WHO guidelines for prediction of aller­genic proteins. P. americana protein sequences were retrieved from UniProtKB. The sequences obtained were analyzed us­ing AlgPred. The potential allergens obtained were further analyzed by SDAP for confirmation."nResults: Protein sequences (233 cases) of P. americana were obtained from UniProtKB out of which 25 were known aller­gens. Out of the remaining 208 proteins, 102 potential allergens were predicted by AlgPred. However, only 9 were found to be potential allergens after screening with SDAP. Arginine kinases, RNA polymerase II subunit, parcxpwnx02, peptidyl-pro­lyl cis-trans isomerase, hemocyanin subunit type I and type II, homologue of Sarcophaga proteinase and alpha amylase  were confirmed to be potential allergens by SDAP."nConclusion: We have identified nine potential allergens in P. americana that may be used as preliminary support for further labo­ratory experiments.  |
| first_indexed | 2024-12-17T08:26:07Z |
| format | Article |
| id | doaj.art-ed992d1455ca4650aa4ad63f6f8682ac |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-17T08:26:07Z |
| publishDate | 2010-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-ed992d1455ca4650aa4ad63f6f8682ac2022-12-21T21:56:46ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852010-09-01393109115In Silico Identification of Potential American Cockroach (Periplaneta americana) AllergensA AhmedK MinhasNamood-e-SaharO AftabF Sher Khan"nBackground: Cockroaches have been recognized as a powerful indoor allergen. Cockroach allergy can be a major factor in seri­ous asthma and nasal allergy. Bioinformatics tools have been developed to identify potential allergens. The present study was conducted to identify potential allergens in Periplaneta americana (Linnaeus)."nMethods: The study focused on the identification of potential allergens among the characterized proteins of P. americana us­ing web-based and publicly available allergen prediction tools that follow the FAO/WHO guidelines for prediction of aller­genic proteins. P. americana protein sequences were retrieved from UniProtKB. The sequences obtained were analyzed us­ing AlgPred. The potential allergens obtained were further analyzed by SDAP for confirmation."nResults: Protein sequences (233 cases) of P. americana were obtained from UniProtKB out of which 25 were known aller­gens. Out of the remaining 208 proteins, 102 potential allergens were predicted by AlgPred. However, only 9 were found to be potential allergens after screening with SDAP. Arginine kinases, RNA polymerase II subunit, parcxpwnx02, peptidyl-pro­lyl cis-trans isomerase, hemocyanin subunit type I and type II, homologue of Sarcophaga proteinase and alpha amylase  were confirmed to be potential allergens by SDAP."nConclusion: We have identified nine potential allergens in P. americana that may be used as preliminary support for further labo­ratory experiments. http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/16702.pdf&manuscript_id=16702AllergenBioinformaticsPredictionPeriplaneta americana |
| spellingShingle | A Ahmed K Minhas Namood-e-Sahar O Aftab F Sher Khan In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens Iranian Journal of Public Health Allergen Bioinformatics Prediction Periplaneta americana |
| title | In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens |
| title_full | In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens |
| title_fullStr | In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens |
| title_full_unstemmed | In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens |
| title_short | In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens |
| title_sort | in silico identification of potential american cockroach periplaneta americana allergens |
| topic | Allergen Bioinformatics Prediction Periplaneta americana |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/16702.pdf&manuscript_id=16702 |
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