Effects of residual H2O2 on the growth of MSCs after decontamination
Introduction: Regenerative therapy is a developing field in medicine. In the production of cell products for these therapies, hygienic management is even more critical than in the production of a chemical drug. At the same time, however, care is required with the use of decontamination agents, consi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Regenerative Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320418300154 |
| _version_ | 1819142016149225472 |
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| author | Riri Chihara Hideki Kitajima Yuuki Ogawa Hiroaki Nakamura Shozo Tsutsui Manabu Mizutani Masahiro Kino-oka Sachikon Ezoe |
| author_facet | Riri Chihara Hideki Kitajima Yuuki Ogawa Hiroaki Nakamura Shozo Tsutsui Manabu Mizutani Masahiro Kino-oka Sachikon Ezoe |
| author_sort | Riri Chihara |
| collection | DOAJ |
| description | Introduction: Regenerative therapy is a developing field in medicine. In the production of cell products for these therapies, hygienic management is even more critical than in the production of a chemical drug. At the same time, however, care is required with the use of decontamination agents, considering their effects on cell viability and characteristics. To date, hydrogen peroxide (H2O2) is most widely used for decontamination in pharmaceutical plants and cell processing facilities. Methods: In this study, we examined the effects of residual H2O2 in the atmosphere of cell processing units after decontamination on the viability and proliferation of mesenchymal stem cells derived from human bone marrow. Results: We detected residual H2O2 sufficient to affect cell proliferation and survival even more than 30 h after decontamination ended. Our results suggest a longer time period is required before starting operations after decontamination and that the operating time should be as short as possible. Conclusions: Here we show the effects of post-decontamination residual H2O2 on the viability and proliferation of mesenchymal stem cells derived from human bone marrow, which may provide us with important information about the hygienic management of cell processing facilities. Keywords: Hydrogen peroxide (H2O2), Hygienic management, Biological safety cabinet (BSC), Messenchymal stem cells (MSCs) |
| first_indexed | 2024-12-22T12:03:38Z |
| format | Article |
| id | doaj.art-ed99485209174856aeb256178b563518 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-22T12:03:38Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-ed99485209174856aeb256178b5635182022-12-21T18:26:30ZengElsevierRegenerative Therapy2352-32042018-12-019111115Effects of residual H2O2 on the growth of MSCs after decontaminationRiri Chihara0Hideki Kitajima1Yuuki Ogawa2Hiroaki Nakamura3Shozo Tsutsui4Manabu Mizutani5Masahiro Kino-oka6Sachikon Ezoe7Development Headquarters, Earth Environmental Service Co., Ltd., 17 Kanda-Konyacho, Chiyodaku, Tokyo, 101-0035, JapanDepartment of Medical Innovation, Osaka University Hospital, 2-1, Yamadaoka, Suita, Osaka, 565-0871, JapanDepartment of Medical Innovation, Osaka University Hospital, 2-1, Yamadaoka, Suita, Osaka, 565-0871, Japan; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, JapanDevelopment Headquarters, Earth Environmental Service Co., Ltd., 17 Kanda-Konyacho, Chiyodaku, Tokyo, 101-0035, JapanDevelopment Headquarters, Earth Environmental Service Co., Ltd., 17 Kanda-Konyacho, Chiyodaku, Tokyo, 101-0035, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, JapanDepartment of Medical Innovation, Osaka University Hospital, 2-1, Yamadaoka, Suita, Osaka, 565-0871, Japan; Department of Environmental Space Infection Control, Graduate School of Medicine, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan; Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan; Corresponding author. Department of Environmental Space Infection Control, Graduate School of Medicine, Osaka University, 1-3, Yamadaoka, Suita, Osaka, 565-0871, Japan. Fax: +81 6 6105 5243.Introduction: Regenerative therapy is a developing field in medicine. In the production of cell products for these therapies, hygienic management is even more critical than in the production of a chemical drug. At the same time, however, care is required with the use of decontamination agents, considering their effects on cell viability and characteristics. To date, hydrogen peroxide (H2O2) is most widely used for decontamination in pharmaceutical plants and cell processing facilities. Methods: In this study, we examined the effects of residual H2O2 in the atmosphere of cell processing units after decontamination on the viability and proliferation of mesenchymal stem cells derived from human bone marrow. Results: We detected residual H2O2 sufficient to affect cell proliferation and survival even more than 30 h after decontamination ended. Our results suggest a longer time period is required before starting operations after decontamination and that the operating time should be as short as possible. Conclusions: Here we show the effects of post-decontamination residual H2O2 on the viability and proliferation of mesenchymal stem cells derived from human bone marrow, which may provide us with important information about the hygienic management of cell processing facilities. Keywords: Hydrogen peroxide (H2O2), Hygienic management, Biological safety cabinet (BSC), Messenchymal stem cells (MSCs)http://www.sciencedirect.com/science/article/pii/S2352320418300154 |
| spellingShingle | Riri Chihara Hideki Kitajima Yuuki Ogawa Hiroaki Nakamura Shozo Tsutsui Manabu Mizutani Masahiro Kino-oka Sachikon Ezoe Effects of residual H2O2 on the growth of MSCs after decontamination Regenerative Therapy |
| title | Effects of residual H2O2 on the growth of MSCs after decontamination |
| title_full | Effects of residual H2O2 on the growth of MSCs after decontamination |
| title_fullStr | Effects of residual H2O2 on the growth of MSCs after decontamination |
| title_full_unstemmed | Effects of residual H2O2 on the growth of MSCs after decontamination |
| title_short | Effects of residual H2O2 on the growth of MSCs after decontamination |
| title_sort | effects of residual h2o2 on the growth of mscs after decontamination |
| url | http://www.sciencedirect.com/science/article/pii/S2352320418300154 |
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