One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be inju...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=2;spage=458;epage=464;aulast= |
| _version_ | 1797738494798331904 |
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| author | Lin Feng Da Li Yao Tian Chengshun Zhao Yun Sun Xiaolong Kou Jun Wu Liu Wang Qi Gu Wei Li Jie Hao Baoyang Hu Yukai Wang |
| author_facet | Lin Feng Da Li Yao Tian Chengshun Zhao Yun Sun Xiaolong Kou Jun Wu Liu Wang Qi Gu Wei Li Jie Hao Baoyang Hu Yukai Wang |
| author_sort | Lin Feng |
| collection | DOAJ |
| description | Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation. |
| first_indexed | 2024-03-12T13:44:36Z |
| format | Article |
| id | doaj.art-ed99c5af3e594bedb444190a9a50efcf |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-03-12T13:44:36Z |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-ed99c5af3e594bedb444190a9a50efcf2023-08-23T09:46:19ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742024-01-0119245846410.4103/1673-5374.377412One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivoLin FengDa LiYao TianChengshun ZhaoYun SunXiaolong KouJun WuLiu WangQi GuWei LiJie HaoBaoyang HuYukai WangNumerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease. Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease. However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells. These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion, effectively maintaining axonal integrity in vitro. Importantly, midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts. Overall, our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=2;spage=458;epage=464;aulast=axonal integrity; cell cryopreservation; cellular environment; cellular niche; cell replacement therapy; dopaminergic progenitors; human pluripotent stem cell; mechanical damage; neuronal cell delivery; parkinson’s disease; small-aperture gelatin microcarriers |
| spellingShingle | Lin Feng Da Li Yao Tian Chengshun Zhao Yun Sun Xiaolong Kou Jun Wu Liu Wang Qi Gu Wei Li Jie Hao Baoyang Hu Yukai Wang One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Neural Regeneration Research axonal integrity; cell cryopreservation; cellular environment; cellular niche; cell replacement therapy; dopaminergic progenitors; human pluripotent stem cell; mechanical damage; neuronal cell delivery; parkinson’s disease; small-aperture gelatin microcarriers |
| title | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| title_full | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| title_fullStr | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| title_full_unstemmed | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| title_short | One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| title_sort | one step cell biomanufacturing platform porous gelatin microcarrier beads promote human embryonic stem cell derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo |
| topic | axonal integrity; cell cryopreservation; cellular environment; cellular niche; cell replacement therapy; dopaminergic progenitors; human pluripotent stem cell; mechanical damage; neuronal cell delivery; parkinson’s disease; small-aperture gelatin microcarriers |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=2;spage=458;epage=464;aulast= |
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