Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis
Traditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/10/5/1097 |
| _version_ | 1797413424712384512 |
|---|---|
| author | Jeong-Ju Yoo Hee Bong Shin Ju Sun Song Minjung Kim Jina Yun Zisun Kim Yoo Min Lee Sang Wook Lee Kwang Woo Lee Woong bin Kim Chang Beom Ryu Sung-Woo Park Seong Kyu Park Ho-Yeon Song Young Ho Kim on the behalf of SMS (Soonchunhyang microbiome multi-disciplinary study group) |
| author_facet | Jeong-Ju Yoo Hee Bong Shin Ju Sun Song Minjung Kim Jina Yun Zisun Kim Yoo Min Lee Sang Wook Lee Kwang Woo Lee Woong bin Kim Chang Beom Ryu Sung-Woo Park Seong Kyu Park Ho-Yeon Song Young Ho Kim on the behalf of SMS (Soonchunhyang microbiome multi-disciplinary study group) |
| author_sort | Jeong-Ju Yoo |
| collection | DOAJ |
| description | Traditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we compared urine NGS results and urine cultures in patients with acute uncomplicated cystitis (AUC) and recurrent cystitis (RC), and evaluated the difference in microbiome patterns in the NGS results. Patients who underwent urine culture and NGS due to AUC or RC were retrospectively reviewed. All urine samples were collected via a transurethral catheter and studied utilizing a type of NGS called 16S ribosomal RNA gene amplification and sequencing. The sensitivity of urine NGS was significantly higher than that of conventional urine culture (69.0% vs. 16.7%, <i>p</i> < 0.05). The detection rate of urine NGS was slightly lower in the RC group than in the AUC group (67.7% vs. 72.7%). Microbiome diversity was significantly higher in the RC group compared to the AUC group (<i>p</i> = 0.007), and the microbiome composition was significantly different between the AUC and RC groups. In the urine NGS results, <i>Pseudomonas, Acinetobacter</i>, and Enterobacteriaceae were found in the AUC group, and <i>Sphingomonas</i>, <i>Staphylococcus, Streptococcus,</i> and <i>Rothia</i> spp. were detected in the RC group. Urine NGS can significantly increase the diagnostic sensitivity compared to traditional urine culture methods, especially in RC patients. AUC and RC patients had significant differences in bacterial diversity and patterns. Therefore, recurrent cystitis might be approached from a different perspective. |
| first_indexed | 2024-03-09T05:17:40Z |
| format | Article |
| id | doaj.art-ed9a3b39e16c4fe1b4606b74aabf6162 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T05:17:40Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-ed9a3b39e16c4fe1b4606b74aabf61622023-12-03T12:44:19ZengMDPI AGJournal of Clinical Medicine2077-03832021-03-01105109710.3390/jcm10051097Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent CystitisJeong-Ju Yoo0Hee Bong Shin1Ju Sun Song2Minjung Kim3Jina Yun4Zisun Kim5Yoo Min Lee6Sang Wook Lee7Kwang Woo Lee8Woong bin Kim9Chang Beom Ryu10Sung-Woo Park11Seong Kyu Park12Ho-Yeon Song13Young Ho Kim14on the behalf of SMS (Soonchunhyang microbiome multi-disciplinary study group)Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Laboratory Medicine and Genetics, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaGC Genome, Green Cross Laboratories, Department of Laboratory Medicine, Yongin 16924, KoreaGC Genome, Green Cross Laboratories, Department of Laboratory Medicine, Yongin 16924, KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Surgery, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Pediatrics, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaDepartment of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 31151, KoreaDepartment of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, KoreaTraditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we compared urine NGS results and urine cultures in patients with acute uncomplicated cystitis (AUC) and recurrent cystitis (RC), and evaluated the difference in microbiome patterns in the NGS results. Patients who underwent urine culture and NGS due to AUC or RC were retrospectively reviewed. All urine samples were collected via a transurethral catheter and studied utilizing a type of NGS called 16S ribosomal RNA gene amplification and sequencing. The sensitivity of urine NGS was significantly higher than that of conventional urine culture (69.0% vs. 16.7%, <i>p</i> < 0.05). The detection rate of urine NGS was slightly lower in the RC group than in the AUC group (67.7% vs. 72.7%). Microbiome diversity was significantly higher in the RC group compared to the AUC group (<i>p</i> = 0.007), and the microbiome composition was significantly different between the AUC and RC groups. In the urine NGS results, <i>Pseudomonas, Acinetobacter</i>, and Enterobacteriaceae were found in the AUC group, and <i>Sphingomonas</i>, <i>Staphylococcus, Streptococcus,</i> and <i>Rothia</i> spp. were detected in the RC group. Urine NGS can significantly increase the diagnostic sensitivity compared to traditional urine culture methods, especially in RC patients. AUC and RC patients had significant differences in bacterial diversity and patterns. Therefore, recurrent cystitis might be approached from a different perspective.https://www.mdpi.com/2077-0383/10/5/109716S rRNA next-generation sequencingcystitisurinary tract infectionmicrobiome |
| spellingShingle | Jeong-Ju Yoo Hee Bong Shin Ju Sun Song Minjung Kim Jina Yun Zisun Kim Yoo Min Lee Sang Wook Lee Kwang Woo Lee Woong bin Kim Chang Beom Ryu Sung-Woo Park Seong Kyu Park Ho-Yeon Song Young Ho Kim on the behalf of SMS (Soonchunhyang microbiome multi-disciplinary study group) Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis Journal of Clinical Medicine 16S rRNA next-generation sequencing cystitis urinary tract infection microbiome |
| title | Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis |
| title_full | Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis |
| title_fullStr | Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis |
| title_full_unstemmed | Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis |
| title_short | Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis |
| title_sort | urinary microbiome characteristics in female patients with acute uncomplicated cystitis and recurrent cystitis |
| topic | 16S rRNA next-generation sequencing cystitis urinary tract infection microbiome |
| url | https://www.mdpi.com/2077-0383/10/5/1097 |
| work_keys_str_mv | AT jeongjuyoo urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT heebongshin urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT jusunsong urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT minjungkim urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT jinayun urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT zisunkim urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT yoominlee urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT sangwooklee urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT kwangwoolee urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT woongbinkim urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT changbeomryu urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT sungwoopark urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT seongkyupark urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT hoyeonsong urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT younghokim urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis AT onthebehalfofsmssoonchunhyangmicrobiomemultidisciplinarystudygroup urinarymicrobiomecharacteristicsinfemalepatientswithacuteuncomplicatedcystitisandrecurrentcystitis |