Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy
Canine visceral leishmaniasis (CVL) due to Leishmania infantum infection is a zoonotic disease prevalent in the areas of South America and the Mediterranean. Infected dogs as reservoirs can contribute to disease transmission and can be a scourge to public health. Therefore, early diagnosis of infect...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.914477/full |
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| author | Sarfaraz Ahmad Ejazi Samiran Saha Anirban Bhattacharyya Sonali Das Nathália Lopes Fontoura Mateus Manoel Sebastião da Costa Lima Herintha Coeto Neitzke-Abreu Ivete Lopes de Mendonca Carlos Henrique Nery Costa Otoni Alves de Oliveira Melo Marcia Almeida de Melo Bartira Rossi-Bergmann Raffaele Corso Laura Manna Nahid Ali |
| author_facet | Sarfaraz Ahmad Ejazi Samiran Saha Anirban Bhattacharyya Sonali Das Nathália Lopes Fontoura Mateus Manoel Sebastião da Costa Lima Herintha Coeto Neitzke-Abreu Ivete Lopes de Mendonca Carlos Henrique Nery Costa Otoni Alves de Oliveira Melo Marcia Almeida de Melo Bartira Rossi-Bergmann Raffaele Corso Laura Manna Nahid Ali |
| author_sort | Sarfaraz Ahmad Ejazi |
| collection | DOAJ |
| description | Canine visceral leishmaniasis (CVL) due to Leishmania infantum infection is a zoonotic disease prevalent in the areas of South America and the Mediterranean. Infected dogs as reservoirs can contribute to disease transmission and can be a scourge to public health. Therefore, early diagnosis of infected dogs may play a pivotal role in circumscribing disease progression. Invasive tissue aspiration and insufficient serological methods impair a single assay for prompt CVL diagnosis. In the present study, we aimed to evaluate the potential of Leishmania donovani isolated membrane protein, LAg, for the diagnosis of CVL through immunological assays. Initially, enzyme-linked immunosorbent assay was done with Brazilian dog sera to evaluate the performance of LAg in diagnosing CVL and found sensitivity and specificity of 92.50% and 95%, respectively. The study further confirmed the diagnostic efficacy of LAg in a dipstick format. The dipstick test of canine sera from three centers in Brazil and one center in Italy collectively showed sensitivity values in the range of 53.33% to 100% in recognizing symptomatic dogs and specificity values between 75% and 100% to rule out healthy dogs. Moreover, a rapid immunochromatographic test was developed and optimized using LAg. This test was able to identify 94.73% of CVL of Brazilian origin with specificity of 97.29%. The current results highlight the reactive potential of the L. donovani antigen, LAg, for L. infantum CVL diagnosis and support our previous findings, which suggest the utility of LAg for the diagnosis of both L. donovani and L. infantum human VL in a variety of endemic regions. LAg as a diagnostic candidate may be employed to identify comprehensive CVL cases in epidemiological areas. |
| first_indexed | 2024-04-13T12:31:14Z |
| format | Article |
| id | doaj.art-ed9b2ca292b1462ca05c020142ef353f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T12:31:14Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-ed9b2ca292b1462ca05c020142ef353f2022-12-22T02:46:51ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-07-011210.3389/fcimb.2022.914477914477Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and ItalySarfaraz Ahmad Ejazi0Samiran Saha1Anirban Bhattacharyya2Sonali Das3Nathália Lopes Fontoura Mateus4Manoel Sebastião da Costa Lima5Herintha Coeto Neitzke-Abreu6Ivete Lopes de Mendonca7Carlos Henrique Nery Costa8Otoni Alves de Oliveira Melo9Marcia Almeida de Melo10Bartira Rossi-Bergmann11Raffaele Corso12Laura Manna13Nahid Ali14CSIR-Indian Institute of Chemical Biology, Kolkata, IndiaCSIR-Indian Institute of Chemical Biology, Kolkata, IndiaCSIR-Indian Institute of Chemical Biology, Kolkata, IndiaCSIR-Indian Institute of Chemical Biology, Kolkata, IndiaFaculty of Veterinary Medicine and Animal Science, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, BrazilAggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, BrazilFaculty of Health Sciences, Universidade Federal da Grande Dourados (UFGD), Mato Grosso do Sul, BrazilDepartamento de Clínica e Cirurgia Veterinária, Universidade Federal do Piaui (UFPI), Teresina, BrazilDepartamento de Clínica e Cirurgia Veterinária, Universidade Federal do Piaui (UFPI), Teresina, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilPrograma de Pós-Graduação em Ciência e Saude Animal, Universidade Federal de Campina Grande (UFCG), Campina Grande, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilPrevention Department, Distretto Sanitario 12, Unità Operativa di Prevenzione Collettiva, Azienda Sanitaria Locale Caserta, Caserta, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples, Federico II, Naples, ItalyCSIR-Indian Institute of Chemical Biology, Kolkata, IndiaCanine visceral leishmaniasis (CVL) due to Leishmania infantum infection is a zoonotic disease prevalent in the areas of South America and the Mediterranean. Infected dogs as reservoirs can contribute to disease transmission and can be a scourge to public health. Therefore, early diagnosis of infected dogs may play a pivotal role in circumscribing disease progression. Invasive tissue aspiration and insufficient serological methods impair a single assay for prompt CVL diagnosis. In the present study, we aimed to evaluate the potential of Leishmania donovani isolated membrane protein, LAg, for the diagnosis of CVL through immunological assays. Initially, enzyme-linked immunosorbent assay was done with Brazilian dog sera to evaluate the performance of LAg in diagnosing CVL and found sensitivity and specificity of 92.50% and 95%, respectively. The study further confirmed the diagnostic efficacy of LAg in a dipstick format. The dipstick test of canine sera from three centers in Brazil and one center in Italy collectively showed sensitivity values in the range of 53.33% to 100% in recognizing symptomatic dogs and specificity values between 75% and 100% to rule out healthy dogs. Moreover, a rapid immunochromatographic test was developed and optimized using LAg. This test was able to identify 94.73% of CVL of Brazilian origin with specificity of 97.29%. The current results highlight the reactive potential of the L. donovani antigen, LAg, for L. infantum CVL diagnosis and support our previous findings, which suggest the utility of LAg for the diagnosis of both L. donovani and L. infantum human VL in a variety of endemic regions. LAg as a diagnostic candidate may be employed to identify comprehensive CVL cases in epidemiological areas.https://www.frontiersin.org/articles/10.3389/fcimb.2022.914477/fullLeishmaniaserologyimmunochromatographyic test (ICT)diagnosiscanine |
| spellingShingle | Sarfaraz Ahmad Ejazi Samiran Saha Anirban Bhattacharyya Sonali Das Nathália Lopes Fontoura Mateus Manoel Sebastião da Costa Lima Herintha Coeto Neitzke-Abreu Ivete Lopes de Mendonca Carlos Henrique Nery Costa Otoni Alves de Oliveira Melo Marcia Almeida de Melo Bartira Rossi-Bergmann Raffaele Corso Laura Manna Nahid Ali Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy Frontiers in Cellular and Infection Microbiology Leishmania serology immunochromatographyic test (ICT) diagnosis canine |
| title | Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy |
| title_full | Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy |
| title_fullStr | Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy |
| title_full_unstemmed | Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy |
| title_short | Development of Immunological Assays Based on Leishmania donovani Antigen for Diagnosis of Canine Visceral Leishmaniasis and Their Multicenter Evaluation in Brazil and Italy |
| title_sort | development of immunological assays based on leishmania donovani antigen for diagnosis of canine visceral leishmaniasis and their multicenter evaluation in brazil and italy |
| topic | Leishmania serology immunochromatographyic test (ICT) diagnosis canine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.914477/full |
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