The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study
Equine fertilization cannot be performed in the laboratory as equine spermatozoa do not cross the oocyte's zona pellucida in vitro. Hence, a more profound study of equine oviductal fluid (OF) composition at the pre-ovulatory and post-ovulatory stages could help in understanding what components...
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| Format: | Article |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.694247/full |
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| author | Pablo Fernández-Hernández Pablo Fernández-Hernández Federica Marinaro María Jesús Sánchez-Calabuig Luis Jesús García-Marín Luis Jesús García-Marín María Julia Bragado María Julia Bragado Lauro González-Fernández Lauro González-Fernández Beatriz Macías-García Beatriz Macías-García |
| author_facet | Pablo Fernández-Hernández Pablo Fernández-Hernández Federica Marinaro María Jesús Sánchez-Calabuig Luis Jesús García-Marín Luis Jesús García-Marín María Julia Bragado María Julia Bragado Lauro González-Fernández Lauro González-Fernández Beatriz Macías-García Beatriz Macías-García |
| author_sort | Pablo Fernández-Hernández |
| collection | DOAJ |
| description | Equine fertilization cannot be performed in the laboratory as equine spermatozoa do not cross the oocyte's zona pellucida in vitro. Hence, a more profound study of equine oviductal fluid (OF) composition at the pre-ovulatory and post-ovulatory stages could help in understanding what components are required to achieve fertilization in horses. Our work aimed to elucidate the proteomic composition of equine OF at both stages. To do this, OF was obtained postmortem from oviducts of slaughtered mares ipsilateral to a pre-ovulatory follicle (n = 4) or a recent ovulation (n = 4); the samples were kept at −80°C until analysis. After protein extraction and isobaric tags for relative and absolute quantification (iTRAQ) labeling, the samples were analyzed by nano-liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The analysis of the spectra resulted in the identification of a total of 1,173 proteins present in pre-ovulatory and post-ovulatory samples; among these, 691 were unique for Equus caballus. Proteins from post-ovulatory oviductal fluid were compared with the proteins from pre-ovulatory oviductal fluid and were categorized as upregulated (positive log fold change) or downregulated (negative log fold change). Fifteen proteins were found to be downregulated in the post-ovulatory fluid and 156 were upregulated in the post-ovulatory OF compared to the pre-ovulatory fluid; among the upregulated proteins, 87 were included in the metabolism of proteins pathway. The identified proteins were related to sperm–oviduct interaction, fertilization, and metabolism, among others. Our data reveal consistent differences in the proteome of equine OF prior to and after ovulation, helping to increase our understanding in the factors that promote fertilization and early embryo development in horses. |
| first_indexed | 2024-12-17T12:48:32Z |
| format | Article |
| id | doaj.art-ed9d88a403534222a53be63eca5a7da8 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-17T12:48:32Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ed9d88a403534222a53be63eca5a7da82022-12-21T21:47:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-08-01810.3389/fvets.2021.694247694247The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative StudyPablo Fernández-Hernández0Pablo Fernández-Hernández1Federica Marinaro2María Jesús Sánchez-Calabuig3Luis Jesús García-Marín4Luis Jesús García-Marín5María Julia Bragado6María Julia Bragado7Lauro González-Fernández8Lauro González-Fernández9Beatriz Macías-García10Beatriz Macías-García11Research Group of Intracellular Signaling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, SpainDepartment of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, SpainStem Cell Therapy Unit, Jesús Usón Minimally Invasive Surgery Centre, Cáceres, SpainDepartment of Animal Medicine and Surgery, Faculty of Veterinary Sciences, University Complutense of Madrid, Madrid, SpainResearch Group of Intracellular Signaling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, SpainDepartment of Physiology, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, SpainResearch Group of Intracellular Signaling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, SpainDepartment of Biochemistry and Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, SpainResearch Group of Intracellular Signaling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, SpainDepartment of Biochemistry and Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, SpainResearch Group of Intracellular Signaling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, SpainDepartment of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, SpainEquine fertilization cannot be performed in the laboratory as equine spermatozoa do not cross the oocyte's zona pellucida in vitro. Hence, a more profound study of equine oviductal fluid (OF) composition at the pre-ovulatory and post-ovulatory stages could help in understanding what components are required to achieve fertilization in horses. Our work aimed to elucidate the proteomic composition of equine OF at both stages. To do this, OF was obtained postmortem from oviducts of slaughtered mares ipsilateral to a pre-ovulatory follicle (n = 4) or a recent ovulation (n = 4); the samples were kept at −80°C until analysis. After protein extraction and isobaric tags for relative and absolute quantification (iTRAQ) labeling, the samples were analyzed by nano-liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The analysis of the spectra resulted in the identification of a total of 1,173 proteins present in pre-ovulatory and post-ovulatory samples; among these, 691 were unique for Equus caballus. Proteins from post-ovulatory oviductal fluid were compared with the proteins from pre-ovulatory oviductal fluid and were categorized as upregulated (positive log fold change) or downregulated (negative log fold change). Fifteen proteins were found to be downregulated in the post-ovulatory fluid and 156 were upregulated in the post-ovulatory OF compared to the pre-ovulatory fluid; among the upregulated proteins, 87 were included in the metabolism of proteins pathway. The identified proteins were related to sperm–oviduct interaction, fertilization, and metabolism, among others. Our data reveal consistent differences in the proteome of equine OF prior to and after ovulation, helping to increase our understanding in the factors that promote fertilization and early embryo development in horses.https://www.frontiersin.org/articles/10.3389/fvets.2021.694247/fullhorseoviductal fluidovulationmass spectrometryproteomedomestic animal reproduction |
| spellingShingle | Pablo Fernández-Hernández Pablo Fernández-Hernández Federica Marinaro María Jesús Sánchez-Calabuig Luis Jesús García-Marín Luis Jesús García-Marín María Julia Bragado María Julia Bragado Lauro González-Fernández Lauro González-Fernández Beatriz Macías-García Beatriz Macías-García The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study Frontiers in Veterinary Science horse oviductal fluid ovulation mass spectrometry proteome domestic animal reproduction |
| title | The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study |
| title_full | The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study |
| title_fullStr | The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study |
| title_full_unstemmed | The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study |
| title_short | The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study |
| title_sort | proteome of equine oviductal fluid varies before and after ovulation a comparative study |
| topic | horse oviductal fluid ovulation mass spectrometry proteome domestic animal reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.694247/full |
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