Silica-Based Nanoparticles for Protein Encapsulation and Delivery
Although conceptually obvious, the effective delivery of proteins in therapeutic applications is far from being a routine practice. The major limitation is the conservation of protein physicochemical identity during the transport to the target site. In this regard, nanoparticle-based systems offer n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-11-01
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| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/8/11/886 |
| _version_ | 1828848550327353344 |
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| author | Filippo Begarani Domenico Cassano Eleonora Margheritis Roberto Marotta Francesco Cardarelli Valerio Voliani |
| author_facet | Filippo Begarani Domenico Cassano Eleonora Margheritis Roberto Marotta Francesco Cardarelli Valerio Voliani |
| author_sort | Filippo Begarani |
| collection | DOAJ |
| description | Although conceptually obvious, the effective delivery of proteins in therapeutic applications is far from being a routine practice. The major limitation is the conservation of protein physicochemical identity during the transport to the target site. In this regard, nanoparticle-based systems offer new intriguing possibilities, provided that (i) the harsh and denaturating conditions typically used for nanoparticle synthesis are avoided or mitigated; and (ii) nanoparticle biocompatibility and degradation (for protein release) are optimized. Here, we tackle these issues by starting from a nanoparticle architecture already tested for small chemical compounds. In particular, silica-shielded liposomes are produced and loaded with a test protein (i.e., Green Fluorescent Protein) in an aqueous environment. We demonstrate promising results concerning protein encapsulation, protection during intracellular trafficking and final release triggered by nanoparticle degradations in acidic organelles. We believe this proof of principle may open new applications and developments for targeted and efficient protein delivery. |
| first_indexed | 2024-12-12T22:32:21Z |
| format | Article |
| id | doaj.art-ed9d91150afc49b0b50ebb92e3e2c11e |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-12T22:32:21Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ed9d91150afc49b0b50ebb92e3e2c11e2022-12-22T00:09:35ZengMDPI AGNanomaterials2079-49912018-11-0181188610.3390/nano8110886nano8110886Silica-Based Nanoparticles for Protein Encapsulation and DeliveryFilippo Begarani0Domenico Cassano1Eleonora Margheritis2Roberto Marotta3Francesco Cardarelli4Valerio Voliani5NEST—Scuola Normale Superiore, Pisa 56100, ItalyNEST—Scuola Normale Superiore, Pisa 56100, ItalyCenter for Nanotechnology Innovation, Istituto Italiano di Tecnologia, Pisa 56100, ItalyElectron Microscopy Facility, Istituto Italiano di Tecnologia, Genova 00161, ItalyNEST—Scuola Normale Superiore, Istituto di Nanoscienze-CNR, Pisa 56100, ItalyCenter for Nanotechnology Innovation, Istituto Italiano di Tecnologia, Pisa 56100, ItalyAlthough conceptually obvious, the effective delivery of proteins in therapeutic applications is far from being a routine practice. The major limitation is the conservation of protein physicochemical identity during the transport to the target site. In this regard, nanoparticle-based systems offer new intriguing possibilities, provided that (i) the harsh and denaturating conditions typically used for nanoparticle synthesis are avoided or mitigated; and (ii) nanoparticle biocompatibility and degradation (for protein release) are optimized. Here, we tackle these issues by starting from a nanoparticle architecture already tested for small chemical compounds. In particular, silica-shielded liposomes are produced and loaded with a test protein (i.e., Green Fluorescent Protein) in an aqueous environment. We demonstrate promising results concerning protein encapsulation, protection during intracellular trafficking and final release triggered by nanoparticle degradations in acidic organelles. We believe this proof of principle may open new applications and developments for targeted and efficient protein delivery.https://www.mdpi.com/2079-4991/8/11/886protein-deliverysilicabiodegradationliposomesLysosomal Storage Disorders (LSDs) |
| spellingShingle | Filippo Begarani Domenico Cassano Eleonora Margheritis Roberto Marotta Francesco Cardarelli Valerio Voliani Silica-Based Nanoparticles for Protein Encapsulation and Delivery Nanomaterials protein-delivery silica biodegradation liposomes Lysosomal Storage Disorders (LSDs) |
| title | Silica-Based Nanoparticles for Protein Encapsulation and Delivery |
| title_full | Silica-Based Nanoparticles for Protein Encapsulation and Delivery |
| title_fullStr | Silica-Based Nanoparticles for Protein Encapsulation and Delivery |
| title_full_unstemmed | Silica-Based Nanoparticles for Protein Encapsulation and Delivery |
| title_short | Silica-Based Nanoparticles for Protein Encapsulation and Delivery |
| title_sort | silica based nanoparticles for protein encapsulation and delivery |
| topic | protein-delivery silica biodegradation liposomes Lysosomal Storage Disorders (LSDs) |
| url | https://www.mdpi.com/2079-4991/8/11/886 |
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