Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae)
Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatom...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/7/640 |
| _version_ | 1827687546195279872 |
|---|---|
| author | Natalia R. Moyetta Fabián O. Ramos Jimena Leyria Lilián E. Canavoso Leonardo L. Fruttero |
| author_facet | Natalia R. Moyetta Fabián O. Ramos Jimena Leyria Lilián E. Canavoso Leonardo L. Fruttero |
| author_sort | Natalia R. Moyetta |
| collection | DOAJ |
| description | Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine <i>Dipetalogaster maxima</i>. Moreover, in this work we describe, for the first time, the ultrastructural features of <i>D. maxima</i> hemocytes. Using phase contrast microscopy of fresh preparations, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers. |
| first_indexed | 2024-03-10T09:36:48Z |
| format | Article |
| id | doaj.art-ed9e85bf14824764afd22e48ddee0b91 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T09:36:48Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-ed9e85bf14824764afd22e48ddee0b912023-11-22T04:04:48ZengMDPI AGInsects2075-44502021-07-0112764010.3390/insects12070640Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae)Natalia R. Moyetta0Fabián O. Ramos1Jimena Leyria2Lilián E. Canavoso3Leonardo L. Fruttero4Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, ArgentinaDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, ArgentinaDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, ArgentinaDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, ArgentinaDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba 5000, ArgentinaHemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine <i>Dipetalogaster maxima</i>. Moreover, in this work we describe, for the first time, the ultrastructural features of <i>D. maxima</i> hemocytes. Using phase contrast microscopy of fresh preparations, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.https://www.mdpi.com/2075-4450/12/7/640hemocytetriatominaemorphologyultrastructureclassification |
| spellingShingle | Natalia R. Moyetta Fabián O. Ramos Jimena Leyria Lilián E. Canavoso Leonardo L. Fruttero Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) Insects hemocyte triatominae morphology ultrastructure classification |
| title | Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) |
| title_full | Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) |
| title_fullStr | Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) |
| title_full_unstemmed | Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) |
| title_short | Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous <i>Dipetalogaster maxima</i> (Hemiptera: Reduviidae) |
| title_sort | morphological and ultrastructural characterization of hemocytes in an insect model the hematophagous i dipetalogaster maxima i hemiptera reduviidae |
| topic | hemocyte triatominae morphology ultrastructure classification |
| url | https://www.mdpi.com/2075-4450/12/7/640 |
| work_keys_str_mv | AT nataliarmoyetta morphologicalandultrastructuralcharacterizationofhemocytesinaninsectmodelthehematophagousidipetalogastermaximaihemipterareduviidae AT fabianoramos morphologicalandultrastructuralcharacterizationofhemocytesinaninsectmodelthehematophagousidipetalogastermaximaihemipterareduviidae AT jimenaleyria morphologicalandultrastructuralcharacterizationofhemocytesinaninsectmodelthehematophagousidipetalogastermaximaihemipterareduviidae AT lilianecanavoso morphologicalandultrastructuralcharacterizationofhemocytesinaninsectmodelthehematophagousidipetalogastermaximaihemipterareduviidae AT leonardolfruttero morphologicalandultrastructuralcharacterizationofhemocytesinaninsectmodelthehematophagousidipetalogastermaximaihemipterareduviidae |