A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer
Nowadays, analytical techniques are moving towards the development of smart biosensing strategies for the point-of-care accurate screening of disease biomarkers, such as human epididymis protein 4 (HE4), a recently discovered serum marker for early ovarian cancer diagnosis. In this context, the pres...
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MDPI AG
2020-04-01
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Online Access: | https://www.mdpi.com/1424-8220/20/7/2016 |
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author | Valentina Bianchi Monica Mattarozzi Marco Giannetto Andrea Boni Ilaria De Munari Maria Careri |
author_facet | Valentina Bianchi Monica Mattarozzi Marco Giannetto Andrea Boni Ilaria De Munari Maria Careri |
author_sort | Valentina Bianchi |
collection | DOAJ |
description | Nowadays, analytical techniques are moving towards the development of smart biosensing strategies for the point-of-care accurate screening of disease biomarkers, such as human epididymis protein 4 (HE4), a recently discovered serum marker for early ovarian cancer diagnosis. In this context, the present work represents the first implementation of a competitive enzyme-labelled magneto-immunoassay exploiting a homemade IoT Wi-Fi cloud-based portable potentiostat for differential pulse voltammetry readout. The electrochemical device was specifically designed to be capable of autonomous calibration and data processing, switching between calibration, and measurement modes: in particular, firstly, a baseline estimation algorithm is applied for correct peak computation, then calibration function is built by interpolating data with a four-parameter logistic function. The calibration function parameters are stored on the cloud for inverse prediction to determine the concentration of unknown samples. Interpolation function calibration and concentration evaluation are performed directly on-board, thus reducing the power consumption. The analytical device was validated in human serum, demonstrating good sensing performance for analysis of HE4 with detection and quantitation limits in human serum of 3.5 and 29.2 pM, respectively, reaching the sensitivity that is required for diagnostic purposes, with high potential for applications as portable and smart diagnostic tool for point-of-care testing. |
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issn | 1424-8220 |
language | English |
last_indexed | 2024-03-10T20:41:30Z |
publishDate | 2020-04-01 |
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spelling | doaj.art-ed9f2027b8904526980141f80aa9d0a52023-11-19T20:39:08ZengMDPI AGSensors1424-82202020-04-01207201610.3390/s20072016A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian CancerValentina Bianchi0Monica Mattarozzi1Marco Giannetto2Andrea Boni3Ilaria De Munari4Maria Careri5Department of Engineering and Architecture, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Engineering and Architecture, University of Parma, 43124 Parma, ItalyDepartment of Engineering and Architecture, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyNowadays, analytical techniques are moving towards the development of smart biosensing strategies for the point-of-care accurate screening of disease biomarkers, such as human epididymis protein 4 (HE4), a recently discovered serum marker for early ovarian cancer diagnosis. In this context, the present work represents the first implementation of a competitive enzyme-labelled magneto-immunoassay exploiting a homemade IoT Wi-Fi cloud-based portable potentiostat for differential pulse voltammetry readout. The electrochemical device was specifically designed to be capable of autonomous calibration and data processing, switching between calibration, and measurement modes: in particular, firstly, a baseline estimation algorithm is applied for correct peak computation, then calibration function is built by interpolating data with a four-parameter logistic function. The calibration function parameters are stored on the cloud for inverse prediction to determine the concentration of unknown samples. Interpolation function calibration and concentration evaluation are performed directly on-board, thus reducing the power consumption. The analytical device was validated in human serum, demonstrating good sensing performance for analysis of HE4 with detection and quantitation limits in human serum of 3.5 and 29.2 pM, respectively, reaching the sensitivity that is required for diagnostic purposes, with high potential for applications as portable and smart diagnostic tool for point-of-care testing.https://www.mdpi.com/1424-8220/20/7/2016human epididymis protein 4competitive electrochemical immunosensorWiFi portable potentiostaton-board calibrationInternet of Things |
spellingShingle | Valentina Bianchi Monica Mattarozzi Marco Giannetto Andrea Boni Ilaria De Munari Maria Careri A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer Sensors human epididymis protein 4 competitive electrochemical immunosensor WiFi portable potentiostat on-board calibration Internet of Things |
title | A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer |
title_full | A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer |
title_fullStr | A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer |
title_full_unstemmed | A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer |
title_short | A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer |
title_sort | self calibrating iot portable electrochemical immunosensor for serum human epididymis protein 4 as a tumor biomarker for ovarian cancer |
topic | human epididymis protein 4 competitive electrochemical immunosensor WiFi portable potentiostat on-board calibration Internet of Things |
url | https://www.mdpi.com/1424-8220/20/7/2016 |
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