In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines
The present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflamm...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
2019-09-01
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| Series: | Brazilian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | http://rbmv.org/index.php/BJVM/article/view/1014 |
| _version_ | 1819022043715207168 |
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| author | Samira Maria Achkar Elaine Raniero Fernandes Fernanda Guedes Enio Mori Carla Isabel Macedo Maria Irma e Seixas Duarte |
| author_facet | Samira Maria Achkar Elaine Raniero Fernandes Fernanda Guedes Enio Mori Carla Isabel Macedo Maria Irma e Seixas Duarte |
| author_sort | Samira Maria Achkar |
| collection | DOAJ |
| description | The present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings. |
| first_indexed | 2024-12-21T04:16:44Z |
| format | Article |
| id | doaj.art-eda3594c7f5b4d1b8435e1b222155703 |
| institution | Directory Open Access Journal |
| issn | 0100-2430 2527-2179 |
| language | English |
| last_indexed | 2024-12-21T04:16:44Z |
| publishDate | 2019-09-01 |
| publisher | Sociedade de Medicina Veterinária do Estado do Rio de Janeiro |
| record_format | Article |
| series | Brazilian Journal of Veterinary Medicine |
| spelling | doaj.art-eda3594c7f5b4d1b8435e1b2221557032022-12-21T19:16:18ZengSociedade de Medicina Veterinária do Estado do Rio de JaneiroBrazilian Journal of Veterinary Medicine0100-24302527-21792019-09-0141e101419e10141910.29374/2527-2179.bjvm1014191014In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovinesSamira Maria Achkar0Elaine Raniero Fernandes1Fernanda Guedes2Enio Mori3Carla Isabel Macedo4Maria Irma e Seixas Duarte5Instituto Pasteur, São Paulo-SP, BrazilInstituto Pasteur, São Paulo-SP, BrazilInstituto Pasteur, São Paulo-SP, BrazilInstituto Pasteur, São Paulo-SP, BrazilInstituto Pasteur, São Paulo-SP, BrazilUniversidade de São Paulo, São Paulo-SP, BrazilThe present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings.http://rbmv.org/index.php/BJVM/article/view/1014keywords: rabies, equines, bovines, immunohistochemistry, fat, histopathological changes |
| spellingShingle | Samira Maria Achkar Elaine Raniero Fernandes Fernanda Guedes Enio Mori Carla Isabel Macedo Maria Irma e Seixas Duarte In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines Brazilian Journal of Veterinary Medicine keywords: rabies, equines, bovines, immunohistochemistry, fat, histopathological changes |
| title | In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| title_full | In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| title_fullStr | In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| title_full_unstemmed | In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| title_short | In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| title_sort | in situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
| topic | keywords: rabies, equines, bovines, immunohistochemistry, fat, histopathological changes |
| url | http://rbmv.org/index.php/BJVM/article/view/1014 |
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