Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy
We have reported that selenium (Se) provided to grazing beef cattle in an inorganic (ISe) form versus a 1:1 mixture (MIX) of inorganic and organic (OSe) forms affects cholesterol biosynthesis in the corpus luteum (CL), the abundance of interferon tau (IFNτ) and progesterone (P4)-induced mRNAs in the...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17327 |
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| author | Sarah N. Carr Benjamin R. Crites Harshraj Shinde Phillip J. Bridges |
| author_facet | Sarah N. Carr Benjamin R. Crites Harshraj Shinde Phillip J. Bridges |
| author_sort | Sarah N. Carr |
| collection | DOAJ |
| description | We have reported that selenium (Se) provided to grazing beef cattle in an inorganic (ISe) form versus a 1:1 mixture (MIX) of inorganic and organic (OSe) forms affects cholesterol biosynthesis in the corpus luteum (CL), the abundance of interferon tau (IFNτ) and progesterone (P4)-induced mRNAs in the caruncular (CAR) tissue of the endometrium, and conceptus length at maternal recognition of pregnancy (MRP). In this study, beef heifers were supplemented with a vitamin–mineral mix containing 35 ppm Se as ISe or MIX to achieve a Se-adequate status. Inseminated heifers were killed at MRP (d 17, n = 6 per treatment) for tissue collection. In CAR samples from MIX versus ISe heifers, qPCR revealed that mRNA encoding the thyroid regulating DIO2 and DIO3 was decreased (<i>p</i> < 0.05) and a complete transcriptomic analysis revealed effects on the interferon JAK-STAT1/2 pathway, including decreased expression of mRNAs encoding the classical interferon stimulated genes IFIT1, IFIT2, IFIT3, IRF1, IRF9, ISG15, OAS2, and RSAD2 (<i>p</i> < 0.05). Treatment also affected the abundance of mRNAs contributing to the immunotolerant environment (<i>p</i> < 0.05). In combination, these findings suggest more advanced preparation of the CAR and developing conceptus for implantation and to evade immune rejection by the maternal system in MIX- vs. ISe-treated heifers. |
| first_indexed | 2024-03-08T20:42:18Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:42:18Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eda639cf055945fdaf14bcc2120fa6132023-12-22T14:14:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241732710.3390/ijms242417327Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of PregnancySarah N. Carr0Benjamin R. Crites1Harshraj Shinde2Phillip J. Bridges3Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USADepartment of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USADepartment of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USADepartment of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USAWe have reported that selenium (Se) provided to grazing beef cattle in an inorganic (ISe) form versus a 1:1 mixture (MIX) of inorganic and organic (OSe) forms affects cholesterol biosynthesis in the corpus luteum (CL), the abundance of interferon tau (IFNτ) and progesterone (P4)-induced mRNAs in the caruncular (CAR) tissue of the endometrium, and conceptus length at maternal recognition of pregnancy (MRP). In this study, beef heifers were supplemented with a vitamin–mineral mix containing 35 ppm Se as ISe or MIX to achieve a Se-adequate status. Inseminated heifers were killed at MRP (d 17, n = 6 per treatment) for tissue collection. In CAR samples from MIX versus ISe heifers, qPCR revealed that mRNA encoding the thyroid regulating DIO2 and DIO3 was decreased (<i>p</i> < 0.05) and a complete transcriptomic analysis revealed effects on the interferon JAK-STAT1/2 pathway, including decreased expression of mRNAs encoding the classical interferon stimulated genes IFIT1, IFIT2, IFIT3, IRF1, IRF9, ISG15, OAS2, and RSAD2 (<i>p</i> < 0.05). Treatment also affected the abundance of mRNAs contributing to the immunotolerant environment (<i>p</i> < 0.05). In combination, these findings suggest more advanced preparation of the CAR and developing conceptus for implantation and to evade immune rejection by the maternal system in MIX- vs. ISe-treated heifers.https://www.mdpi.com/1422-0067/24/24/17327seleniumselenoproteinscaruncleendometriummaternal recognition of pregnancyinterferon tau |
| spellingShingle | Sarah N. Carr Benjamin R. Crites Harshraj Shinde Phillip J. Bridges Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy International Journal of Molecular Sciences selenium selenoproteins caruncle endometrium maternal recognition of pregnancy interferon tau |
| title | Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy |
| title_full | Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy |
| title_fullStr | Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy |
| title_full_unstemmed | Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy |
| title_short | Transcriptomic Changes in Response to Form of Selenium on the Interferon-Tau Signaling Mechanism in the Caruncular Tissue of Beef Heifers at Maternal Recognition of Pregnancy |
| title_sort | transcriptomic changes in response to form of selenium on the interferon tau signaling mechanism in the caruncular tissue of beef heifers at maternal recognition of pregnancy |
| topic | selenium selenoproteins caruncle endometrium maternal recognition of pregnancy interferon tau |
| url | https://www.mdpi.com/1422-0067/24/24/17327 |
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