RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer
In the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (...
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| Format: | Article |
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Elsevier
2003-07-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558603800295 |
| _version_ | 1818127448983732224 |
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| author | Jie Li Zhongqiu Zhang Zunyan Dai Anthony P. Popkie Christoph Plass Carl Morrison Yian Wang Ming You |
| author_facet | Jie Li Zhongqiu Zhang Zunyan Dai Anthony P. Popkie Christoph Plass Carl Morrison Yian Wang Ming You |
| author_sort | Jie Li |
| collection | DOAJ |
| description | In the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (10 of 33) of adenocarcinomas, 25% (3 of 12) of large cell carcinomas, and only 5% (1 of 20) of squamous cell carcinomas contained activated Kras2 mutation at codon 12 or 13. RASSF1A promoter region CpG island methylation was detected in adenocarcinomas (55%), large cell carcinomas (25%), and squamous cell carcinomas (25%). Interestingly, combined RASSF1A methylation and Kras2 mutation data show that only ~7% adenocarcinomas/large cell carcinomas exhibited both KRASSF1A promoter methylation and Kras2 mutation, whereas 24% adenocarcinomas, 50% large cell carcinomas, and 70% squamous cell carcinomas showed neither Kras2 mutation nor RASSF1A promoter methylation. These results showed that the majority of the primary NSCLCs with Kras2 mutations lack RASSF1A inactivation, and both RASSF1A inactivation and Kras2 mutation events occur frequently in adenocarcinomas and large cell carcinomas. Our results indicate a trend of inverse relationship between Kras2 activation and RASSF1A promoter methylation in the majority of human lung adenocarcinomas and large cell carcinomas. |
| first_indexed | 2024-12-11T07:17:32Z |
| format | Article |
| id | doaj.art-edab9ea4c31746c099dfa54e9a582d32 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-11T07:17:32Z |
| publishDate | 2003-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-edab9ea4c31746c099dfa54e9a582d322022-12-22T01:16:11ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022003-07-015436236610.1016/S1476-5586(03)80029-5RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung CancerJie Li0Zhongqiu Zhang1Zunyan Dai2Anthony P. Popkie3Christoph Plass4Carl Morrison5Yian Wang6Ming You7Department of Surgery and the Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USADepartment of Surgery and the Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USAThe Ohio State University Comprehensive Cancer Center, Columbus, OH, USAThe Ohio State University Comprehensive Cancer Center, Columbus, OH, USAThe Ohio State University Comprehensive Cancer Center, Columbus, OH, USAThe Ohio State University Comprehensive Cancer Center, Columbus, OH, USADepartment of Surgery and the Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USADepartment of Surgery and the Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USAIn the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (10 of 33) of adenocarcinomas, 25% (3 of 12) of large cell carcinomas, and only 5% (1 of 20) of squamous cell carcinomas contained activated Kras2 mutation at codon 12 or 13. RASSF1A promoter region CpG island methylation was detected in adenocarcinomas (55%), large cell carcinomas (25%), and squamous cell carcinomas (25%). Interestingly, combined RASSF1A methylation and Kras2 mutation data show that only ~7% adenocarcinomas/large cell carcinomas exhibited both KRASSF1A promoter methylation and Kras2 mutation, whereas 24% adenocarcinomas, 50% large cell carcinomas, and 70% squamous cell carcinomas showed neither Kras2 mutation nor RASSF1A promoter methylation. These results showed that the majority of the primary NSCLCs with Kras2 mutations lack RASSF1A inactivation, and both RASSF1A inactivation and Kras2 mutation events occur frequently in adenocarcinomas and large cell carcinomas. Our results indicate a trend of inverse relationship between Kras2 activation and RASSF1A promoter methylation in the majority of human lung adenocarcinomas and large cell carcinomas.http://www.sciencedirect.com/science/article/pii/S1476558603800295Kras2RASSF1Amutationsmethylationlung cancer |
| spellingShingle | Jie Li Zhongqiu Zhang Zunyan Dai Anthony P. Popkie Christoph Plass Carl Morrison Yian Wang Ming You RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer Neoplasia: An International Journal for Oncology Research Kras2 RASSF1A mutations methylation lung cancer |
| title | RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer |
| title_full | RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer |
| title_fullStr | RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer |
| title_full_unstemmed | RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer |
| title_short | RASSF1A Promoter Methylation and Kras2 Mutations in Non Small Cell Lung Cancer |
| title_sort | rassf1a promoter methylation and kras2 mutations in non small cell lung cancer |
| topic | Kras2 RASSF1A mutations methylation lung cancer |
| url | http://www.sciencedirect.com/science/article/pii/S1476558603800295 |
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