Key wheat GRF genes constraining wheat tillering of mutant dmc
Tillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This st...
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PeerJ Inc.
2021-04-01
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| author | Jing Zhang Junchang Li Yongjing Ni Yumei Jiang Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Mengyao Han Lei Li Hongjie Liu Qiaoyun Li Jishan Niu |
| author_facet | Jing Zhang Junchang Li Yongjing Ni Yumei Jiang Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Mengyao Han Lei Li Hongjie Liu Qiaoyun Li Jishan Niu |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Tillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This study is to systematically investigate the roles of all the wheat GRFs (T. aestivum GRFs, TaGRFs) in regulating tillering, and screen out the key regulators. A total of 30 TaGRFs were identified and their physicochemical properties, gene structures, conserved domains, phylogenetic relationships and tissue expression profiles were analyzed. The expression levels of all the TaGRFs were significantly lower in dmc than those in WT at early tillering stage, and the abnormal expressions of TaGRF2-7(A, B, D), TaGRF5-7D, TaGRF10-6(A, B, D) and TaGRF11-2A were major causes constraining the tillering of dmc. The transcriptions of TaGRFs were significantly affected by exogenous indole acetic acid (IAA) and gibberellin acid (GA3) applications, which suggested that TaGRFs as well as IAA, GA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of GRF genes in wheat. |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:09:51Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-edaf27c36a0d4e6fa811d74b40163f412023-12-03T09:18:08ZengPeerJ Inc.PeerJ2167-83592021-04-019e1123510.7717/peerj.11235Key wheat GRF genes constraining wheat tillering of mutant dmcJing Zhang0Junchang Li1Yongjing Ni2Yumei Jiang3Zhixin Jiao4Huijuan Li5Ting Wang6Peipei Zhang7Mengyao Han8Lei Li9Hongjie Liu10Qiaoyun Li11Jishan Niu12Henan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaShangqiu Academy of Agricultural and Forestry Sciences, Shangqiu, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaShangqiu Academy of Agricultural and Forestry Sciences, Shangqiu, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaHenan Agricultural University, National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, Henan, ChinaTillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This study is to systematically investigate the roles of all the wheat GRFs (T. aestivum GRFs, TaGRFs) in regulating tillering, and screen out the key regulators. A total of 30 TaGRFs were identified and their physicochemical properties, gene structures, conserved domains, phylogenetic relationships and tissue expression profiles were analyzed. The expression levels of all the TaGRFs were significantly lower in dmc than those in WT at early tillering stage, and the abnormal expressions of TaGRF2-7(A, B, D), TaGRF5-7D, TaGRF10-6(A, B, D) and TaGRF11-2A were major causes constraining the tillering of dmc. The transcriptions of TaGRFs were significantly affected by exogenous indole acetic acid (IAA) and gibberellin acid (GA3) applications, which suggested that TaGRFs as well as IAA, GA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of GRF genes in wheat.https://peerj.com/articles/11235.pdfWheat (Triticum aestivum L.)TilleringGrowth regulating factorExpression profilesIAAGA |
| spellingShingle | Jing Zhang Junchang Li Yongjing Ni Yumei Jiang Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Mengyao Han Lei Li Hongjie Liu Qiaoyun Li Jishan Niu Key wheat GRF genes constraining wheat tillering of mutant dmc PeerJ Wheat (Triticum aestivum L.) Tillering Growth regulating factor Expression profiles IAA GA |
| title | Key wheat GRF genes constraining wheat tillering of mutant dmc |
| title_full | Key wheat GRF genes constraining wheat tillering of mutant dmc |
| title_fullStr | Key wheat GRF genes constraining wheat tillering of mutant dmc |
| title_full_unstemmed | Key wheat GRF genes constraining wheat tillering of mutant dmc |
| title_short | Key wheat GRF genes constraining wheat tillering of mutant dmc |
| title_sort | key wheat grf genes constraining wheat tillering of mutant dmc |
| topic | Wheat (Triticum aestivum L.) Tillering Growth regulating factor Expression profiles IAA GA |
| url | https://peerj.com/articles/11235.pdf |
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