Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China
Shigella flexneri serotype 2 variant (II:3,4,7,8) was isolated in 2008 and first reported in China in 2013. In the present study, epidemiological surveillance from 2003 to 2013 in China suggested that this serotype first appeared in Guangxi in 2003; it then emerged in Shanghai and Xinjiang in 2004 a...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00435/full |
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| author | Xianyan eCui Xianyan eCui Jian eWang Chaojie eYang Beibei eLiang Qiuxia eMa Shengjie eYi Hao eLi Hongbo eLiu Peng eLi Zhihao eWu Jing eXie Leili eJia Rongzhang eHao Ligui eWang Yuejin eHua Shaofu eQiu Hongbin eSong |
| author_facet | Xianyan eCui Xianyan eCui Jian eWang Chaojie eYang Beibei eLiang Qiuxia eMa Shengjie eYi Hao eLi Hongbo eLiu Peng eLi Zhihao eWu Jing eXie Leili eJia Rongzhang eHao Ligui eWang Yuejin eHua Shaofu eQiu Hongbin eSong |
| author_sort | Xianyan eCui |
| collection | DOAJ |
| description | Shigella flexneri serotype 2 variant (II:3,4,7,8) was isolated in 2008 and first reported in China in 2013. In the present study, epidemiological surveillance from 2003 to 2013 in China suggested that this serotype first appeared in Guangxi in 2003; it then emerged in Shanghai and Xinjiang in 2004 and in Henan in 2008. Of the 1813 S. flexneri isolates, 58 S. flexneri serotype 2 variant strains were identified. Serotype 2 variant has emerged as a prominent serotype in recent years, with 2a (32.6%), X variant (25.2%), 1a (9.4%), X (6.3%), 2b (5.4%), and 1b (3.6%). According to phenotypic and genotypic analysis, the serotype 2 variant originated from 2a and 2b. A higher antibiotic resistance rate was observed between 2009 and 2013 than that between 2003 and 2008. Among 22 cephalosporin-resistant isolates, blaTEM-1, blaOXA-1, blaCTX-3, blaCTX-14, and blaCTX-79 were detected. Among 22 fluoroquinolone-resistant isolates, a Ser80Ile mutation in parC was present in all of the isolates. Moreover, 21 isolates had three gyrA point mutations (Ser83Leu, His211Tyr, Asp87Asn or Gly) and one isolate had two gyrA point mutations (Ser83Leu and His211Tyr). The prevalence of His211Tyr in the fluoroquinolone-resistant isolates is concerning, and the mutation was first reported in China. Besides, 22 isolates harbored the aac(6')-Ib-cr gene, and two isolates harbored qnrS1. In view of the increased epidemic frequency and multidrug-resistant strain emergence, continuous surveillance will be needed to understand the actual disease burden and provide guidance for shigellosis. |
| first_indexed | 2024-12-19T17:17:06Z |
| format | Article |
| id | doaj.art-edaffd66a356447abb5499d47d1b8bfb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T17:17:06Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-edaffd66a356447abb5499d47d1b8bfb2022-12-21T20:12:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-05-01610.3389/fmicb.2015.00435140945Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in ChinaXianyan eCui0Xianyan eCui1Jian eWang2Chaojie eYang3Beibei eLiang4Qiuxia eMa5Shengjie eYi6Hao eLi7Hongbo eLiu8Peng eLi9Zhihao eWu10Jing eXie11Leili eJia12Rongzhang eHao13Ligui eWang14Yuejin eHua15Shaofu eQiu16Hongbin eSong17Zhejiang UniversityInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesZhejiang UniversityInstitute of Disease Control and Prevention, Academy of Military Medical SciencesInstitute of Disease Control and Prevention, Academy of Military Medical SciencesShigella flexneri serotype 2 variant (II:3,4,7,8) was isolated in 2008 and first reported in China in 2013. In the present study, epidemiological surveillance from 2003 to 2013 in China suggested that this serotype first appeared in Guangxi in 2003; it then emerged in Shanghai and Xinjiang in 2004 and in Henan in 2008. Of the 1813 S. flexneri isolates, 58 S. flexneri serotype 2 variant strains were identified. Serotype 2 variant has emerged as a prominent serotype in recent years, with 2a (32.6%), X variant (25.2%), 1a (9.4%), X (6.3%), 2b (5.4%), and 1b (3.6%). According to phenotypic and genotypic analysis, the serotype 2 variant originated from 2a and 2b. A higher antibiotic resistance rate was observed between 2009 and 2013 than that between 2003 and 2008. Among 22 cephalosporin-resistant isolates, blaTEM-1, blaOXA-1, blaCTX-3, blaCTX-14, and blaCTX-79 were detected. Among 22 fluoroquinolone-resistant isolates, a Ser80Ile mutation in parC was present in all of the isolates. Moreover, 21 isolates had three gyrA point mutations (Ser83Leu, His211Tyr, Asp87Asn or Gly) and one isolate had two gyrA point mutations (Ser83Leu and His211Tyr). The prevalence of His211Tyr in the fluoroquinolone-resistant isolates is concerning, and the mutation was first reported in China. Besides, 22 isolates harbored the aac(6')-Ib-cr gene, and two isolates harbored qnrS1. In view of the increased epidemic frequency and multidrug-resistant strain emergence, continuous surveillance will be needed to understand the actual disease burden and provide guidance for shigellosis.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00435/fullShigella flexneriMultidrug-resistantEpidemic frequencyserotype 2 variantantibiotic resistance rate |
| spellingShingle | Xianyan eCui Xianyan eCui Jian eWang Chaojie eYang Beibei eLiang Qiuxia eMa Shengjie eYi Hao eLi Hongbo eLiu Peng eLi Zhihao eWu Jing eXie Leili eJia Rongzhang eHao Ligui eWang Yuejin eHua Shaofu eQiu Hongbin eSong Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China Frontiers in Microbiology Shigella flexneri Multidrug-resistant Epidemic frequency serotype 2 variant antibiotic resistance rate |
| title | Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China |
| title_full | Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China |
| title_fullStr | Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China |
| title_full_unstemmed | Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China |
| title_short | Prevalence and antimicrobial resistance of Shigella flexneri serotype 2 variant in China |
| title_sort | prevalence and antimicrobial resistance of shigella flexneri serotype 2 variant in china |
| topic | Shigella flexneri Multidrug-resistant Epidemic frequency serotype 2 variant antibiotic resistance rate |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00435/full |
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