Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile
In Chile, studies of parasites from the family Sarcocystidae (Apicomplexa) have mostly been related to domestic animals. We aimed to assess the presence of Sarcocystidae taxa in cricetid rodents from Central and Southern Chile. We studied 207 rodents, encompassing six species, from 13 localities. We...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/13/2100 |
| _version_ | 1797592236083380224 |
|---|---|
| author | Pablo Oyarzún-Ruiz Richard S. Thomas Adriana M. Santodomingo Juan E. Uribe Marlon M. Ardila Diana M. Echeverry Sebastián Muñoz-Leal María C. Silva-de la Fuente Marco Loyola Cristina J. Palma Carlos Landaeta-Aqueveque AnaLía Henríquez |
| author_facet | Pablo Oyarzún-Ruiz Richard S. Thomas Adriana M. Santodomingo Juan E. Uribe Marlon M. Ardila Diana M. Echeverry Sebastián Muñoz-Leal María C. Silva-de la Fuente Marco Loyola Cristina J. Palma Carlos Landaeta-Aqueveque AnaLía Henríquez |
| author_sort | Pablo Oyarzún-Ruiz |
| collection | DOAJ |
| description | In Chile, studies of parasites from the family Sarcocystidae (Apicomplexa) have mostly been related to domestic animals. We aimed to assess the presence of Sarcocystidae taxa in cricetid rodents from Central and Southern Chile. We studied 207 rodents, encompassing six species, from 13 localities. We isolated DNA from tissue samples, amplified the Sarcocystidae 18S rRNA gene with polymerase chain reaction, and performed phylogenetic analyses using maximum likelihood and Bayesian inferences. In addition, we examined blood smears and performed histological studies in organs from Sarcocystidae DNA-positive animals. Three specimens were DNA-positive and three genotypes were retrieved and named: <i>Sarcocystis</i> sp. P61, related to <i>Sarcocystis strixi</i>, was detected in two <i>Abrothrix olivacea</i>. Toxoplasmatinae gen. sp. P99 was retrieved from those same two specimens, and was related to <i>Toxoplasma</i> and other genera, although it branched independently. <i>Besnoitia</i> sp. R34 was detected in one <i>Abrothrix hirta</i>, and was clustered with congeneric species associated with rodents. No protozoa were found during microscopic studies; thus, it was not possible to confirm parasitic interactions rather than accidental encounters. However, the close relatedness of the retrieved genotypes to parasites of rodents supports the hypothesis of host–parasite associations. All three genotypes are suggested as potential new taxa, including a putative new genus. |
| first_indexed | 2024-03-11T01:49:34Z |
| format | Article |
| id | doaj.art-edb0bc684bba41c895be7c159bd1debc |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:49:34Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-edb0bc684bba41c895be7c159bd1debc2023-11-18T16:02:58ZengMDPI AGAnimals2076-26152023-06-011313210010.3390/ani13132100Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern ChilePablo Oyarzún-Ruiz0Richard S. Thomas1Adriana M. Santodomingo2Juan E. Uribe3Marlon M. Ardila4Diana M. Echeverry5Sebastián Muñoz-Leal6María C. Silva-de la Fuente7Marco Loyola8Cristina J. Palma9Carlos Landaeta-Aqueveque10AnaLía Henríquez11Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileDepartamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (MNCN-CSIC), 28006 Madrid, SpainFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias de la Naturaleza, Universidad San Sebastián, Concepción 4080871, ChileFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Curicó 3340000, ChileFacultad de Ciencias de la Naturaleza, Universidad San Sebastián, Concepción 4080871, ChileFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, ChileFacultad de Ciencias de la Naturaleza, Universidad San Sebastián, Concepción 4080871, ChileIn Chile, studies of parasites from the family Sarcocystidae (Apicomplexa) have mostly been related to domestic animals. We aimed to assess the presence of Sarcocystidae taxa in cricetid rodents from Central and Southern Chile. We studied 207 rodents, encompassing six species, from 13 localities. We isolated DNA from tissue samples, amplified the Sarcocystidae 18S rRNA gene with polymerase chain reaction, and performed phylogenetic analyses using maximum likelihood and Bayesian inferences. In addition, we examined blood smears and performed histological studies in organs from Sarcocystidae DNA-positive animals. Three specimens were DNA-positive and three genotypes were retrieved and named: <i>Sarcocystis</i> sp. P61, related to <i>Sarcocystis strixi</i>, was detected in two <i>Abrothrix olivacea</i>. Toxoplasmatinae gen. sp. P99 was retrieved from those same two specimens, and was related to <i>Toxoplasma</i> and other genera, although it branched independently. <i>Besnoitia</i> sp. R34 was detected in one <i>Abrothrix hirta</i>, and was clustered with congeneric species associated with rodents. No protozoa were found during microscopic studies; thus, it was not possible to confirm parasitic interactions rather than accidental encounters. However, the close relatedness of the retrieved genotypes to parasites of rodents supports the hypothesis of host–parasite associations. All three genotypes are suggested as potential new taxa, including a putative new genus.https://www.mdpi.com/2076-2615/13/13/2100molecular characterization<i>Abrothrix</i>CoccidiaCricetidae<i>Besnoitia</i>phylogeny |
| spellingShingle | Pablo Oyarzún-Ruiz Richard S. Thomas Adriana M. Santodomingo Juan E. Uribe Marlon M. Ardila Diana M. Echeverry Sebastián Muñoz-Leal María C. Silva-de la Fuente Marco Loyola Cristina J. Palma Carlos Landaeta-Aqueveque AnaLía Henríquez Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile Animals molecular characterization <i>Abrothrix</i> Coccidia Cricetidae <i>Besnoitia</i> phylogeny |
| title | Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile |
| title_full | Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile |
| title_fullStr | Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile |
| title_full_unstemmed | Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile |
| title_short | Survey and Molecular Characterization of Sarcocystidae protozoa in Wild Cricetid Rodents from Central and Southern Chile |
| title_sort | survey and molecular characterization of sarcocystidae protozoa in wild cricetid rodents from central and southern chile |
| topic | molecular characterization <i>Abrothrix</i> Coccidia Cricetidae <i>Besnoitia</i> phylogeny |
| url | https://www.mdpi.com/2076-2615/13/13/2100 |
| work_keys_str_mv | AT pablooyarzunruiz surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT richardsthomas surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT adrianamsantodomingo surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT juaneuribe surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT marlonmardila surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT dianamecheverry surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT sebastianmunozleal surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT mariacsilvadelafuente surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT marcoloyola surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT cristinajpalma surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT carloslandaetaaqueveque surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile AT analiahenriquez surveyandmolecularcharacterizationofsarcocystidaeprotozoainwildcricetidrodentsfromcentralandsouthernchile |