Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham
Euphorbia prostrata (E. prostrata) and Crotalaria burhia (C. burhia) are widely found in flora of the Cholistan Desert of Bahawalpur, Pakistan and are traditionally used to treat pain and chronic disease. The current study aimed to evaluate their phytochemical screening, antioxidant activity, in-viv...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024033103 |
| _version_ | 1797224302110572544 |
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| author | Muhammad Rahil Aslam Hafiz Muhammad Asif Abdulaziz Alamri Adnan Ahmed Bhutto Muhammad Mukhtiar Khalil Ahmad Mehak Muhammad Ashfaq Hafiz Abdul Sattar Abdul Hayee Sana Jabbar Rabia Zahid Allah Nawaz |
| author_facet | Muhammad Rahil Aslam Hafiz Muhammad Asif Abdulaziz Alamri Adnan Ahmed Bhutto Muhammad Mukhtiar Khalil Ahmad Mehak Muhammad Ashfaq Hafiz Abdul Sattar Abdul Hayee Sana Jabbar Rabia Zahid Allah Nawaz |
| author_sort | Muhammad Rahil Aslam |
| collection | DOAJ |
| description | Euphorbia prostrata (E. prostrata) and Crotalaria burhia (C. burhia) are widely found in flora of the Cholistan Desert of Bahawalpur, Pakistan and are traditionally used to treat pain and chronic disease. The current study aimed to evaluate their phytochemical screening, antioxidant activity, in-vivo phagocytic activity, and analgesic activity. Both the plant extracts were investigated for phytochemical screening, Fourier Transform Infrared (FTIR) analysis, in-vitro antioxidant by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, in-vivo immunomodulatory activity by macrophages phagocytosis using carbon clearance rate assay and analgesic activity by acetic acid produced writhing method. Phytochemical screening showed the presence of carbohydrates, saponins, tannins, phenols, quinines, proteins, terpenes, glycosides, and alkaloids. FTIR analysis revealed the existence of different functional groups in both extracts. The DPPH method showed that E. prostrata exhibited a high antioxidant potential with an IC50 of 62.5 μg/ml whereas C. burhia showed a lower antioxidant potential. At the dose of 200 mg/kg body weight (b. wt), both the extracts showed a significant increase in the phagocytic index by 5.2 ± 0.2, and, 4.8 ± 0.1 (p < 0.001) respectively which was close to the 100 mg/kg b. wt of the standard drug (Levamisole) 5.4 ± 0.2. Both the extracts at the dose of 200 mg/kg b. wt also significantly reduced the writhing (abdominal contractions) count by 13.7 ± 0.3 and, 25.3 ± 1.5 (p < 0.001), showing 71.8% and 47.6% of reduced analgesic activity compared to the standard drug dicloran (diclofenac sodium), respectively. In conclusion, extracts of both plants indicate their role in the management of various disorders to relieve pain and modulate the immune system. |
| first_indexed | 2024-04-24T13:50:57Z |
| format | Article |
| id | doaj.art-edb14bdfd1be4fffa0389604fd1ded40 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:57Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-edb14bdfd1be4fffa0389604fd1ded402024-04-04T05:04:31ZengElsevierHeliyon2405-84402024-03-01106e27279Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–HamMuhammad Rahil Aslam0Hafiz Muhammad Asif1Abdulaziz Alamri2Adnan Ahmed Bhutto3Muhammad Mukhtiar4Khalil Ahmad5Mehak Muhammad Ashfaq6Hafiz Abdul Sattar7Abdul Hayee8Sana Jabbar9Rabia Zahid10Allah Nawaz11University College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan; First Department of Internal Medicine, Graduate School of Medical & Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, JapanUniversity College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan; Corresponding author.Department of Biochemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; Corresponding author.APPNA Institute of Public Health, Jinnah Sindh Medical University, PakistanDepartment of Pharmacy, The University of Poonch, Rawalakot, AJK, PakistanUniversity College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, PakistanCollege of Public Health, Ziaudin University Karachi, PakistanUniversity College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, PakistanDepartment of Immunology, Academic Assembly, University of Toyama, Toyama, JapanLaboratory of Cancer Biology and Immunology, Department of Pharmaceutical Sciences, Institute of Natural Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama Shi, 930-0194, JapanUniversity College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, PakistanJoslin Diabetes Center, Harvard Medical School Boston, Massachusetts, USAEuphorbia prostrata (E. prostrata) and Crotalaria burhia (C. burhia) are widely found in flora of the Cholistan Desert of Bahawalpur, Pakistan and are traditionally used to treat pain and chronic disease. The current study aimed to evaluate their phytochemical screening, antioxidant activity, in-vivo phagocytic activity, and analgesic activity. Both the plant extracts were investigated for phytochemical screening, Fourier Transform Infrared (FTIR) analysis, in-vitro antioxidant by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, in-vivo immunomodulatory activity by macrophages phagocytosis using carbon clearance rate assay and analgesic activity by acetic acid produced writhing method. Phytochemical screening showed the presence of carbohydrates, saponins, tannins, phenols, quinines, proteins, terpenes, glycosides, and alkaloids. FTIR analysis revealed the existence of different functional groups in both extracts. The DPPH method showed that E. prostrata exhibited a high antioxidant potential with an IC50 of 62.5 μg/ml whereas C. burhia showed a lower antioxidant potential. At the dose of 200 mg/kg body weight (b. wt), both the extracts showed a significant increase in the phagocytic index by 5.2 ± 0.2, and, 4.8 ± 0.1 (p < 0.001) respectively which was close to the 100 mg/kg b. wt of the standard drug (Levamisole) 5.4 ± 0.2. Both the extracts at the dose of 200 mg/kg b. wt also significantly reduced the writhing (abdominal contractions) count by 13.7 ± 0.3 and, 25.3 ± 1.5 (p < 0.001), showing 71.8% and 47.6% of reduced analgesic activity compared to the standard drug dicloran (diclofenac sodium), respectively. In conclusion, extracts of both plants indicate their role in the management of various disorders to relieve pain and modulate the immune system.http://www.sciencedirect.com/science/article/pii/S2405844024033103Analgesic effectAntioxidantCrotalaria burhiaEuphorbia prostrataImmunomodulatory |
| spellingShingle | Muhammad Rahil Aslam Hafiz Muhammad Asif Abdulaziz Alamri Adnan Ahmed Bhutto Muhammad Mukhtiar Khalil Ahmad Mehak Muhammad Ashfaq Hafiz Abdul Sattar Abdul Hayee Sana Jabbar Rabia Zahid Allah Nawaz Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham Heliyon Analgesic effect Antioxidant Crotalaria burhia Euphorbia prostrata Immunomodulatory |
| title | Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham |
| title_full | Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham |
| title_fullStr | Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham |
| title_full_unstemmed | Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham |
| title_short | Phytochemical screening, antioxidant, immunomodulatory and analgesic potential of aqueous-ethanolic extracts of Euphorbia prostrata ait. and Crotalaria burhia Buch–Ham |
| title_sort | phytochemical screening antioxidant immunomodulatory and analgesic potential of aqueous ethanolic extracts of euphorbia prostrata ait and crotalaria burhia buch ham |
| topic | Analgesic effect Antioxidant Crotalaria burhia Euphorbia prostrata Immunomodulatory |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024033103 |
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