Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams
Fish side streams represent between 20–80% of the weight of fish, and their disposal represents an environmental and economic problem. These side streams contain proteins and antioxidant compounds that give them added value. In order to valorise these side streams, an extraction assisted by pulsed e...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Biology and Life Sciences Forum |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-9976/6/1/18 |
| _version_ | 1797613170601230336 |
|---|---|
| author | Francisco J. Martí-Quijal Francisco J. Barba Francesc Ramon-Mascarell |
| author_facet | Francisco J. Martí-Quijal Francisco J. Barba Francesc Ramon-Mascarell |
| author_sort | Francisco J. Martí-Quijal |
| collection | DOAJ |
| description | Fish side streams represent between 20–80% of the weight of fish, and their disposal represents an environmental and economic problem. These side streams contain proteins and antioxidant compounds that give them added value. In order to valorise these side streams, an extraction assisted by pulsed electric fields (PEF) was carried out to obtain proteins and compounds with antioxidant activity. An extraction for the same time but without PEF pre-treatment was used as a control. The side streams used were head, skin, viscera and backbone from sea bass. The antioxidant capacity was measured by two complementary methods, TEAC and ORAC. The results showed that in the case of head and skin, treatment with PEF notably improved protein extraction by 37.7 and 37.8%, respectively. However, for viscera, the best values were achieved with the control extraction. Finally, backbone did not show significant differences in protein recovery between PEF-assisted extraction and control extraction. Regarding antioxidant capacity, the results followed the same trend as for proteins. For head and skin, the pre-treatment with PEF increased the extraction of antioxidant compounds, improving the TEAC values by 21.74 and 29.11%, respectively. On the other hand, ORAC results increased 22.11% for head extracts and 40.93% for skin extracts. In the extracts obtained from the viscera, the TEAC and ORAC values were better in the control sample, being 21.43% and 24.82% higher, respectively. The antioxidant compounds present in the samples may be bioactive peptides, but a more exhaustive analysis using chromatographic techniques would be necessary to complete the information. Therefore, it has been seen that PEF-assisted extraction can be a good strategy to increase the recovery of proteins and antioxidant compounds from fish side streams such as head or skin, but not for viscera and backbone. |
| first_indexed | 2024-03-11T06:52:06Z |
| format | Article |
| id | doaj.art-edb2fc3edbff44dfb806b080c7010e24 |
| institution | Directory Open Access Journal |
| issn | 2673-9976 |
| language | English |
| last_indexed | 2024-03-11T06:52:06Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology and Life Sciences Forum |
| spelling | doaj.art-edb2fc3edbff44dfb806b080c7010e242023-11-17T09:57:18ZengMDPI AGBiology and Life Sciences Forum2673-99762021-10-01611810.3390/Foods2021-10962Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side StreamsFrancisco J. Martí-Quijal0Francisco J. Barba1Francesc Ramon-Mascarell2Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, Burjassot, 46100 València, SpainNutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, Burjassot, 46100 València, SpainNutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, Burjassot, 46100 València, SpainFish side streams represent between 20–80% of the weight of fish, and their disposal represents an environmental and economic problem. These side streams contain proteins and antioxidant compounds that give them added value. In order to valorise these side streams, an extraction assisted by pulsed electric fields (PEF) was carried out to obtain proteins and compounds with antioxidant activity. An extraction for the same time but without PEF pre-treatment was used as a control. The side streams used were head, skin, viscera and backbone from sea bass. The antioxidant capacity was measured by two complementary methods, TEAC and ORAC. The results showed that in the case of head and skin, treatment with PEF notably improved protein extraction by 37.7 and 37.8%, respectively. However, for viscera, the best values were achieved with the control extraction. Finally, backbone did not show significant differences in protein recovery between PEF-assisted extraction and control extraction. Regarding antioxidant capacity, the results followed the same trend as for proteins. For head and skin, the pre-treatment with PEF increased the extraction of antioxidant compounds, improving the TEAC values by 21.74 and 29.11%, respectively. On the other hand, ORAC results increased 22.11% for head extracts and 40.93% for skin extracts. In the extracts obtained from the viscera, the TEAC and ORAC values were better in the control sample, being 21.43% and 24.82% higher, respectively. The antioxidant compounds present in the samples may be bioactive peptides, but a more exhaustive analysis using chromatographic techniques would be necessary to complete the information. Therefore, it has been seen that PEF-assisted extraction can be a good strategy to increase the recovery of proteins and antioxidant compounds from fish side streams such as head or skin, but not for viscera and backbone.https://www.mdpi.com/2673-9976/6/1/18fish side streamsproteinsantioxidantpulsed electric fields |
| spellingShingle | Francisco J. Martí-Quijal Francisco J. Barba Francesc Ramon-Mascarell Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams Biology and Life Sciences Forum fish side streams proteins antioxidant pulsed electric fields |
| title | Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams |
| title_full | Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams |
| title_fullStr | Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams |
| title_full_unstemmed | Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams |
| title_short | Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams |
| title_sort | effect of pulsed electric fields on the recovery of antioxidant protein extracts from fish side streams |
| topic | fish side streams proteins antioxidant pulsed electric fields |
| url | https://www.mdpi.com/2673-9976/6/1/18 |
| work_keys_str_mv | AT franciscojmartiquijal effectofpulsedelectricfieldsontherecoveryofantioxidantproteinextractsfromfishsidestreams AT franciscojbarba effectofpulsedelectricfieldsontherecoveryofantioxidantproteinextractsfromfishsidestreams AT francescramonmascarell effectofpulsedelectricfieldsontherecoveryofantioxidantproteinextractsfromfishsidestreams |