Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats
Aaptamine, a natural marine compound isolated from the sea sponge, has various biological activities, including delta-opioid agonist properties. However, the effects of aaptamine in neuropathic pain remain unclear. In the present study, we used a chronic constriction injury (CCI)-induced peripheral...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/21/2/113 |
| _version_ | 1797619724813598720 |
|---|---|
| author | Chun-Sung Sung Hao-Jung Cheng Nan-Fu Chen Shih-Hsuan Tang Hsiao-Mei Kuo Ping-Jyun Sung Wu-Fu Chen Zhi-Hong Wen |
| author_facet | Chun-Sung Sung Hao-Jung Cheng Nan-Fu Chen Shih-Hsuan Tang Hsiao-Mei Kuo Ping-Jyun Sung Wu-Fu Chen Zhi-Hong Wen |
| author_sort | Chun-Sung Sung |
| collection | DOAJ |
| description | Aaptamine, a natural marine compound isolated from the sea sponge, has various biological activities, including delta-opioid agonist properties. However, the effects of aaptamine in neuropathic pain remain unclear. In the present study, we used a chronic constriction injury (CCI)-induced peripheral neuropathic rat model to explore the analgesic effects of intrathecal aaptamine administration. We also investigated cellular angiogenesis and lactate dehydrogenase A (LDHA) expression in the ipsilateral lumbar spinal cord after aaptamine administration in CCI rats by immunohistofluorescence. The results showed that aaptamine alleviates CCI-induced nociceptive sensitization, allodynia, and hyperalgesia. Moreover, aaptamine significantly downregulated CCI-induced vascular endothelial growth factor (VEGF), cluster of differentiation 31 (CD31), and LDHA expression in the spinal cord. Double immunofluorescent staining showed that the spinal VEGF and LDHA majorly expressed on astrocytes and neurons, respectively, in CCI rats and inhibited by aaptamine. Collectively, our results indicate aaptamine’s potential as an analgesic agent for neuropathic pain. Furthermore, inhibition of astrocyte-derived angiogenesis and neuronal LDHA expression might be beneficial in neuropathy. |
| first_indexed | 2024-03-11T08:32:00Z |
| format | Article |
| id | doaj.art-edb3ca4e7ed4490d84d09947888ec309 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T08:32:00Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-edb3ca4e7ed4490d84d09947888ec3092023-11-16T21:47:58ZengMDPI AGMarine Drugs1660-33972023-02-0121211310.3390/md21020113Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in RatsChun-Sung Sung0Hao-Jung Cheng1Nan-Fu Chen2Shih-Hsuan Tang3Hsiao-Mei Kuo4Ping-Jyun Sung5Wu-Fu Chen6Zhi-Hong Wen7Department of Anesthesiology, Division of Pain Management, Taipei Veterans General Hospital, Taipei 112201, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804201, TaiwanDepartment of Surgery, Division of Neurosurgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 802301, TaiwanDepartment of Anesthesiology, Division of Pain Management, Taipei Veterans General Hospital, Taipei 112201, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804201, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804201, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804201, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804201, TaiwanAaptamine, a natural marine compound isolated from the sea sponge, has various biological activities, including delta-opioid agonist properties. However, the effects of aaptamine in neuropathic pain remain unclear. In the present study, we used a chronic constriction injury (CCI)-induced peripheral neuropathic rat model to explore the analgesic effects of intrathecal aaptamine administration. We also investigated cellular angiogenesis and lactate dehydrogenase A (LDHA) expression in the ipsilateral lumbar spinal cord after aaptamine administration in CCI rats by immunohistofluorescence. The results showed that aaptamine alleviates CCI-induced nociceptive sensitization, allodynia, and hyperalgesia. Moreover, aaptamine significantly downregulated CCI-induced vascular endothelial growth factor (VEGF), cluster of differentiation 31 (CD31), and LDHA expression in the spinal cord. Double immunofluorescent staining showed that the spinal VEGF and LDHA majorly expressed on astrocytes and neurons, respectively, in CCI rats and inhibited by aaptamine. Collectively, our results indicate aaptamine’s potential as an analgesic agent for neuropathic pain. Furthermore, inhibition of astrocyte-derived angiogenesis and neuronal LDHA expression might be beneficial in neuropathy.https://www.mdpi.com/1660-3397/21/2/113aaptamineneuropathic painangiogenesisLDHA |
| spellingShingle | Chun-Sung Sung Hao-Jung Cheng Nan-Fu Chen Shih-Hsuan Tang Hsiao-Mei Kuo Ping-Jyun Sung Wu-Fu Chen Zhi-Hong Wen Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats Marine Drugs aaptamine neuropathic pain angiogenesis LDHA |
| title | Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats |
| title_full | Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats |
| title_fullStr | Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats |
| title_full_unstemmed | Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats |
| title_short | Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats |
| title_sort | antinociceptive effects of aaptamine a sponge component on peripheral neuropathy in rats |
| topic | aaptamine neuropathic pain angiogenesis LDHA |
| url | https://www.mdpi.com/1660-3397/21/2/113 |
| work_keys_str_mv | AT chunsungsung antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT haojungcheng antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT nanfuchen antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT shihhsuantang antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT hsiaomeikuo antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT pingjyunsung antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT wufuchen antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats AT zhihongwen antinociceptiveeffectsofaaptamineaspongecomponentonperipheralneuropathyinrats |