Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study
Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Plasma |
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| Online Access: | https://www.mdpi.com/2571-6182/5/2/18 |
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| author | Hideo Fukuhara Endre J. Szili Jun-Seok Oh Kawada Chiaki Shinkuro Yamamoto Atsushi Kurabayashi Mutsuo Furihata Masayuki Tsuda Hiroshi Furuta Howard D. Lindsay Robert D. Short Akimitsu Hatta Keiji Inoue |
| author_facet | Hideo Fukuhara Endre J. Szili Jun-Seok Oh Kawada Chiaki Shinkuro Yamamoto Atsushi Kurabayashi Mutsuo Furihata Masayuki Tsuda Hiroshi Furuta Howard D. Lindsay Robert D. Short Akimitsu Hatta Keiji Inoue |
| author_sort | Hideo Fukuhara |
| collection | DOAJ |
| description | Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pre-treatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer. |
| first_indexed | 2024-03-09T22:43:04Z |
| format | Article |
| id | doaj.art-edb5d4bad2094e63bd214c81c543264d |
| institution | Directory Open Access Journal |
| issn | 2571-6182 |
| language | English |
| last_indexed | 2024-03-09T22:43:04Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plasma |
| spelling | doaj.art-edb5d4bad2094e63bd214c81c543264d2023-11-23T18:35:34ZengMDPI AGPlasma2571-61822022-04-015223324610.3390/plasma5020018Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo StudyHideo Fukuhara0Endre J. Szili1Jun-Seok Oh2Kawada Chiaki3Shinkuro Yamamoto4Atsushi Kurabayashi5Mutsuo Furihata6Masayuki Tsuda7Hiroshi Furuta8Howard D. Lindsay9Robert D. Short10Akimitsu Hatta11Keiji Inoue12Department of Urology, Kochi Medical School, Nankoku 783-8505, JapanFuture Industries Institute, Adelaide, SA 5095, AustraliaDepartment of Physics and Electronics, Graduate School of Engineering, Osaka Metropolitan University, Osaka 558-8585, JapanDepartment of Urology, Kochi Medical School, Nankoku 783-8505, JapanDepartment of Urology, Kochi Medical School, Nankoku 783-8505, JapanDepartment of Pathology, Kochi Medical School, Nankoku 783-8505, JapanDepartment of Pathology, Kochi Medical School, Nankoku 783-8505, JapanDivision of Laboratory Animal Science, Life Sciences and Functional Materials Section, Kochi Medical School, Nankoku 783-8505, JapanDepartment of Electronic & Photonic Systems Engineering, Kochi University of Technology, Kami 782-8502, JapanLancaster Medical School, Lancaster University, Lancaster LA1 4YW, UKDepartment of Chemistry and Material Science Institute, Lancaster University, Lancaster LA1 4YW, UKDepartment of Electronic & Photonic Systems Engineering, Kochi University of Technology, Kami 782-8502, JapanDepartment of Urology, Kochi Medical School, Nankoku 783-8505, JapanCurrent methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pre-treatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.https://www.mdpi.com/2571-6182/5/2/18plasma activated mediabladder cancerreactive oxygen speciesoxidative stresscaspase 3cytochrome c |
| spellingShingle | Hideo Fukuhara Endre J. Szili Jun-Seok Oh Kawada Chiaki Shinkuro Yamamoto Atsushi Kurabayashi Mutsuo Furihata Masayuki Tsuda Hiroshi Furuta Howard D. Lindsay Robert D. Short Akimitsu Hatta Keiji Inoue Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study Plasma plasma activated media bladder cancer reactive oxygen species oxidative stress caspase 3 cytochrome c |
| title | Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study |
| title_full | Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study |
| title_fullStr | Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study |
| title_full_unstemmed | Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study |
| title_short | Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study |
| title_sort | oxidative stress pathways linked to apoptosis induction by low temperature plasma jet activated media in bladder cancer cells an in vitro and in vivo study |
| topic | plasma activated media bladder cancer reactive oxygen species oxidative stress caspase 3 cytochrome c |
| url | https://www.mdpi.com/2571-6182/5/2/18 |
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