Plant cell wall hydrolysis process reveals structure–activity relationships
Abstract Background Recent interest in Populus as a source of renewable energy, combined with its numerous available pretreatment methods, has enabled further research on structural modification and hydrolysis. To improve the biodegradation efficiency of biomass, a better understanding of the relati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-11-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13007-020-00691-5 |
| _version_ | 1818116751028649984 |
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| author | Yanan Zhang Shengnan Xu Fan Ji Yubing Hu Zhongwei Gu Bingqian Xu |
| author_facet | Yanan Zhang Shengnan Xu Fan Ji Yubing Hu Zhongwei Gu Bingqian Xu |
| author_sort | Yanan Zhang |
| collection | DOAJ |
| description | Abstract Background Recent interest in Populus as a source of renewable energy, combined with its numerous available pretreatment methods, has enabled further research on structural modification and hydrolysis. To improve the biodegradation efficiency of biomass, a better understanding of the relationship between its macroscopic structures and enzymatic process is important. Results This study investigated mutant cell wall structures compared with wild type on a molecular level. Furthermore, a novel insight into the structural dynamics occurring on mutant biomass was assessed in situ and in real time by functional Atomic Force Microscopy (AFM) imaging. High-resolution AFM images confirmed that genetic pretreatment effectively inhibited the production of irregular lignin. The average roughness values of the wild type are 78, 60, and 30 nm which are much higher than that of the mutant cell wall, approximately 10 nm. It is shown that the action of endoglucanases would expose pure crystalline cellulose with more cracks for easier hydrolysis by cellobiohydrolase I (CBHI). Throughout the entire CBHI hydrolytic process, when the average roughness exceeded 3 nm, the hydrolysis mode consisted of a peeling action. Conclusion Functional AFM imaging is helpful for biomass structural characterization. In addition, the visualization of the enzymatic hydrolysis process will be useful to explore the cell wall structure–activity relationships. |
| first_indexed | 2024-12-11T04:27:29Z |
| format | Article |
| id | doaj.art-edbcbbd669764ebf9bad53ba7af7e7f5 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-11T04:27:29Z |
| publishDate | 2020-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-edbcbbd669764ebf9bad53ba7af7e7f52022-12-22T01:20:57ZengBMCPlant Methods1746-48112020-11-0116111010.1186/s13007-020-00691-5Plant cell wall hydrolysis process reveals structure–activity relationshipsYanan Zhang0Shengnan Xu1Fan Ji2Yubing Hu3Zhongwei Gu4Bingqian Xu5College of Materials Science and Engineering, Nanjing Tech UniversityCollege of Materials Science and Engineering, Nanjing Tech UniversityCollege of Materials Science and Engineering, Nanjing Tech UniversityNational Special Superfine Powder Engineering Research Center of China, Nanjing University of Science & TechnologyCollege of Materials Science and Engineering, Nanjing Tech UniversitySingle Molecule Study Laboratory, College of Engineering and Nanoscale Science and Engineering Center, University of GeorgiaAbstract Background Recent interest in Populus as a source of renewable energy, combined with its numerous available pretreatment methods, has enabled further research on structural modification and hydrolysis. To improve the biodegradation efficiency of biomass, a better understanding of the relationship between its macroscopic structures and enzymatic process is important. Results This study investigated mutant cell wall structures compared with wild type on a molecular level. Furthermore, a novel insight into the structural dynamics occurring on mutant biomass was assessed in situ and in real time by functional Atomic Force Microscopy (AFM) imaging. High-resolution AFM images confirmed that genetic pretreatment effectively inhibited the production of irregular lignin. The average roughness values of the wild type are 78, 60, and 30 nm which are much higher than that of the mutant cell wall, approximately 10 nm. It is shown that the action of endoglucanases would expose pure crystalline cellulose with more cracks for easier hydrolysis by cellobiohydrolase I (CBHI). Throughout the entire CBHI hydrolytic process, when the average roughness exceeded 3 nm, the hydrolysis mode consisted of a peeling action. Conclusion Functional AFM imaging is helpful for biomass structural characterization. In addition, the visualization of the enzymatic hydrolysis process will be useful to explore the cell wall structure–activity relationships.http://link.springer.com/article/10.1186/s13007-020-00691-5PopulusMutant plant cell wallBiomass degradationCarbohydrate active enzymeAFM imagingReal-time |
| spellingShingle | Yanan Zhang Shengnan Xu Fan Ji Yubing Hu Zhongwei Gu Bingqian Xu Plant cell wall hydrolysis process reveals structure–activity relationships Plant Methods Populus Mutant plant cell wall Biomass degradation Carbohydrate active enzyme AFM imaging Real-time |
| title | Plant cell wall hydrolysis process reveals structure–activity relationships |
| title_full | Plant cell wall hydrolysis process reveals structure–activity relationships |
| title_fullStr | Plant cell wall hydrolysis process reveals structure–activity relationships |
| title_full_unstemmed | Plant cell wall hydrolysis process reveals structure–activity relationships |
| title_short | Plant cell wall hydrolysis process reveals structure–activity relationships |
| title_sort | plant cell wall hydrolysis process reveals structure activity relationships |
| topic | Populus Mutant plant cell wall Biomass degradation Carbohydrate active enzyme AFM imaging Real-time |
| url | http://link.springer.com/article/10.1186/s13007-020-00691-5 |
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