Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant
Xiawei Jiang1, Xinjie Cui1, Wenhong Liu1, Hao Xu2, Beiwen Zheng2 1College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laborator...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-09-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/genetic-characterization-of-a-novel-sequence-type-of-multidrug-resista-peer-reviewed-article-IDR |
| _version_ | 1818349851961720832 |
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| author | Jiang X Cui X Liu W Xu H Zheng B |
| author_facet | Jiang X Cui X Liu W Xu H Zheng B |
| author_sort | Jiang X |
| collection | DOAJ |
| description | Xiawei Jiang1, Xinjie Cui1, Wenhong Liu1, Hao Xu2, Beiwen Zheng2 1College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Beiwen ZhengCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou 310000, People’s Republic of ChinaTel +86 5 718 723 6423Fax +86 5 718 723 6423Email zhengbw@zju.edu.cnAbstract: A multidrug-resistant Citrobacter freundii strain R17 was isolated from a wastewater treatment plant in China. Whole-genome sequencing of strain R17 revealed a new sequence type (ST412) chromosome (length 5,124,258 bp) and an Inc FII (Yp) group plasmid pCFR17_1 (length 206,820 bp). A total of 13 antibiotic-resistance genes (ARGs) that confer resistance to eight different antibiotic groups were encoded by strain R17 and 12 of them were carried by plasmid pCFR17_1. These data and analysis suggest that the environment-derived C. freundii strains may serve as potential sources of ARGs and highlight the need of further surveillance of this bacteria in the future.Keywords: Citrobacter freundii, multidrug resistant, antibiotic-resistance genes, whole-genome sequencing, wastewater treatment plant, insertion elements |
| first_indexed | 2024-12-13T18:12:32Z |
| format | Article |
| id | doaj.art-edbd354a51fb4edf951b99b09a0fd541 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-13T18:12:32Z |
| publishDate | 2019-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-edbd354a51fb4edf951b99b09a0fd5412022-12-21T23:35:55ZengDove Medical PressInfection and Drug Resistance1178-69732019-09-01Volume 122775277948448Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plantJiang XCui XLiu WXu HZheng BXiawei Jiang1, Xinjie Cui1, Wenhong Liu1, Hao Xu2, Beiwen Zheng2 1College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Beiwen ZhengCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou 310000, People’s Republic of ChinaTel +86 5 718 723 6423Fax +86 5 718 723 6423Email zhengbw@zju.edu.cnAbstract: A multidrug-resistant Citrobacter freundii strain R17 was isolated from a wastewater treatment plant in China. Whole-genome sequencing of strain R17 revealed a new sequence type (ST412) chromosome (length 5,124,258 bp) and an Inc FII (Yp) group plasmid pCFR17_1 (length 206,820 bp). A total of 13 antibiotic-resistance genes (ARGs) that confer resistance to eight different antibiotic groups were encoded by strain R17 and 12 of them were carried by plasmid pCFR17_1. These data and analysis suggest that the environment-derived C. freundii strains may serve as potential sources of ARGs and highlight the need of further surveillance of this bacteria in the future.Keywords: Citrobacter freundii, multidrug resistant, antibiotic-resistance genes, whole-genome sequencing, wastewater treatment plant, insertion elementshttps://www.dovepress.com/genetic-characterization-of-a-novel-sequence-type-of-multidrug-resista-peer-reviewed-article-IDRCitrobacter freundiimultidrug resistantantibiotic resistance geneswhole genome sequencingwastewater treatment plantinsertion elements |
| spellingShingle | Jiang X Cui X Liu W Xu H Zheng B Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant Infection and Drug Resistance Citrobacter freundii multidrug resistant antibiotic resistance genes whole genome sequencing wastewater treatment plant insertion elements |
| title | Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant |
| title_full | Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant |
| title_fullStr | Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant |
| title_full_unstemmed | Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant |
| title_short | Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant |
| title_sort | genetic characterization of a novel sequence type of multidrug resistant citrobacter freundii strain recovered from wastewater treatment plant |
| topic | Citrobacter freundii multidrug resistant antibiotic resistance genes whole genome sequencing wastewater treatment plant insertion elements |
| url | https://www.dovepress.com/genetic-characterization-of-a-novel-sequence-type-of-multidrug-resista-peer-reviewed-article-IDR |
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