Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro
Abstract Purpose Dimethyl α‐ketoglutarate (dm‐α‐KG) promotes in vitro development to blastocysts of C57BL/6J X C3He F1 mouse zygotes cultured in medium lacking pyruvate. Here, we examined the effects of pyruvate and dm‐α‐KG on in vitro development to blastocysts of ICR mouse zygotes and their post‐i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12288 |
| _version_ | 1819068043989352448 |
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| author | Eun Sol Choi Koga Kawano Misaki Hiraya Eibai Matsukawa Masayasu Yamada |
| author_facet | Eun Sol Choi Koga Kawano Misaki Hiraya Eibai Matsukawa Masayasu Yamada |
| author_sort | Eun Sol Choi |
| collection | DOAJ |
| description | Abstract Purpose Dimethyl α‐ketoglutarate (dm‐α‐KG) promotes in vitro development to blastocysts of C57BL/6J X C3He F1 mouse zygotes cultured in medium lacking pyruvate. Here, we examined the effects of pyruvate and dm‐α‐KG on in vitro development to blastocysts of ICR mouse zygotes and their post‐implantation developmental ability. Methods Zygotes were cultured in medium with pyruvate at 0‐0.2 mmol/L in the presence or absence of 1 mmol/L dm‐α‐KG for 96 hours and evaluated for blastocyst formation rates. The resultant blastocysts were non‐surgically transferred to surrogates and evaluated for birth rates. Results In medium lacking pyruvate, zygotes could not develop beyond the two‐cell stage, in the presence or absence of dm‐α‐KG. However, the blastocyst formation rate in medium with 0.01 mmol/L pyruvate (12%) was markedly increased with addition of dm‐α‐KG (49%). Around 80% of embryos developed to blastocysts in medium with 0.2 mmol/L pyruvate, in the presence or absence of dm‐α‐KG. Importantly, birth rate was markedly improved by treatment with 0.2 mmol/L pyruvate and dm‐αKG (31.0%), compared with those with pyruvate treatment alone (16.3%). Conclusions Pyruvate and dm‐α‐KG synergistically work during in vitro culture to markedly improve the blastocyst formation rate and post‐implantation developmental ability of the resultant blastocysts in ICR mice. |
| first_indexed | 2024-12-21T16:27:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-12-21T16:27:53Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-edbfc3dba91a4e4da957e96e5eb702a52022-12-21T18:57:25ZengWileyReproductive Medicine and Biology1445-57811447-05782019-10-0118440541010.1002/rmb2.12288Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitroEun Sol Choi0Koga Kawano1Misaki Hiraya2Eibai Matsukawa3Masayasu Yamada4Laboratory of Reproductive Biology, Graduate School of Agriculture Kyoto University Kyoto JapanLaboratory of Reproductive Biology, Graduate School of Agriculture Kyoto University Kyoto JapanLaboratory of Reproductive Biology, Graduate School of Agriculture Kyoto University Kyoto JapanNippon Medical & Chemical Instruments Co., LTD. Osaka JapanLaboratory of Reproductive Biology, Graduate School of Agriculture Kyoto University Kyoto JapanAbstract Purpose Dimethyl α‐ketoglutarate (dm‐α‐KG) promotes in vitro development to blastocysts of C57BL/6J X C3He F1 mouse zygotes cultured in medium lacking pyruvate. Here, we examined the effects of pyruvate and dm‐α‐KG on in vitro development to blastocysts of ICR mouse zygotes and their post‐implantation developmental ability. Methods Zygotes were cultured in medium with pyruvate at 0‐0.2 mmol/L in the presence or absence of 1 mmol/L dm‐α‐KG for 96 hours and evaluated for blastocyst formation rates. The resultant blastocysts were non‐surgically transferred to surrogates and evaluated for birth rates. Results In medium lacking pyruvate, zygotes could not develop beyond the two‐cell stage, in the presence or absence of dm‐α‐KG. However, the blastocyst formation rate in medium with 0.01 mmol/L pyruvate (12%) was markedly increased with addition of dm‐α‐KG (49%). Around 80% of embryos developed to blastocysts in medium with 0.2 mmol/L pyruvate, in the presence or absence of dm‐α‐KG. Importantly, birth rate was markedly improved by treatment with 0.2 mmol/L pyruvate and dm‐αKG (31.0%), compared with those with pyruvate treatment alone (16.3%). Conclusions Pyruvate and dm‐α‐KG synergistically work during in vitro culture to markedly improve the blastocyst formation rate and post‐implantation developmental ability of the resultant blastocysts in ICR mice.https://doi.org/10.1002/rmb2.12288dimethyl α‐ketoglutaratein vitro culturemicepre‐and post‐implantation developmentpyruvate |
| spellingShingle | Eun Sol Choi Koga Kawano Misaki Hiraya Eibai Matsukawa Masayasu Yamada Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro Reproductive Medicine and Biology dimethyl α‐ketoglutarate in vitro culture mice pre‐and post‐implantation development pyruvate |
| title | Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro |
| title_full | Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro |
| title_fullStr | Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro |
| title_full_unstemmed | Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro |
| title_short | Effects of pyruvate and dimethyl‐α‐ketoglutarate, either alone or in combination, on pre‐ and post‐implantation development of mouse zygotes cultured in vitro |
| title_sort | effects of pyruvate and dimethyl α ketoglutarate either alone or in combination on pre and post implantation development of mouse zygotes cultured in vitro |
| topic | dimethyl α‐ketoglutarate in vitro culture mice pre‐and post‐implantation development pyruvate |
| url | https://doi.org/10.1002/rmb2.12288 |
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