Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-05-01
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| Series: | European Journal of Translational Myology |
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| Online Access: | https://pagepressjournals.org/index.php/bam/article/view/10409 |
| _version_ | 1811309399991386112 |
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| author | Bahare Rafiee Saied Karbalay-doust Seyed Mohammad Bagher Tabei Negar Azarpira Sanaz Alaee Parvin Lohrasbi Soghra Bahmanpour |
| author_facet | Bahare Rafiee Saied Karbalay-doust Seyed Mohammad Bagher Tabei Negar Azarpira Sanaz Alaee Parvin Lohrasbi Soghra Bahmanpour |
| author_sort | Bahare Rafiee |
| collection | DOAJ |
| description |
In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-induced PCOS group (n=16), PCOS + metformin (n=16), PCOS+NAC (n=16) and a separate control group for NAC (n=16). Another PCOS group was maintained for a month to make sure that features remain till the end of the study. Testosterone level, vaginal cytology and stereological evaluations were assessed. Vaginal cytology in letrozole-receiving mice showed a diestrus phase continuity. Testosterone level, body weight, uterine weight, endometrial volume, myometrial volume, gland volume, stromal volume, epithelial volume, vessel volume, daughter and conglomerate glands, endometrial thickness, and myometrial thickness exhibited an increasing trend in the uterus of PCOS mice. While normal gland and vessel length decreased in the PCOS group. Ovarian volume, corticomedullary volume, primary follicles, secondary follicles, and ovarian cysts were increased in PCOS ovaries. While corpus luteum, primordial, graafian, and atretic follicles showed a decline in the PCOS group. NAC and metformin, however, managed to restore the condition to normal. Given the prevalence of PCOS and its impact on fertility, the use of noninvasive methods is of crucial significance. NAC can control and treat pathological parameters and help as a harmless drug in the treatment of women with PCOS.
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| first_indexed | 2024-04-13T09:41:27Z |
| format | Article |
| id | doaj.art-edc05a8dcf2740e3b7518135652830b0 |
| institution | Directory Open Access Journal |
| issn | 2037-7452 2037-7460 |
| language | English |
| last_indexed | 2024-04-13T09:41:27Z |
| publishDate | 2022-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Translational Myology |
| spelling | doaj.art-edc05a8dcf2740e3b7518135652830b02022-12-22T02:51:55ZengPAGEPress PublicationsEuropean Journal of Translational Myology2037-74522037-74602022-05-0110.4081/ejtm.2022.10409Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovaryBahare Rafiee0Saied Karbalay-doust1Seyed Mohammad Bagher Tabei2Negar Azarpira3Sanaz Alaee4Parvin Lohrasbi5Soghra Bahmanpour6Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sceineces, ShirazHistomorphometry and Stereology Research Center, Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, ShirazMaternal-Fetal Medicine Research Center, Department of Medical Genetics, School od Msedicine, Shiraz University of Medical Sciences, ShirazTransplant Research Center, Shiraz University of Medical Sciences, ShirazDepartment of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sceineces, ShirazDepartment of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sceineces, ShirazSchool of Medicine, Shiraz University of Medical Sciences, Shiraz In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-induced PCOS group (n=16), PCOS + metformin (n=16), PCOS+NAC (n=16) and a separate control group for NAC (n=16). Another PCOS group was maintained for a month to make sure that features remain till the end of the study. Testosterone level, vaginal cytology and stereological evaluations were assessed. Vaginal cytology in letrozole-receiving mice showed a diestrus phase continuity. Testosterone level, body weight, uterine weight, endometrial volume, myometrial volume, gland volume, stromal volume, epithelial volume, vessel volume, daughter and conglomerate glands, endometrial thickness, and myometrial thickness exhibited an increasing trend in the uterus of PCOS mice. While normal gland and vessel length decreased in the PCOS group. Ovarian volume, corticomedullary volume, primary follicles, secondary follicles, and ovarian cysts were increased in PCOS ovaries. While corpus luteum, primordial, graafian, and atretic follicles showed a decline in the PCOS group. NAC and metformin, however, managed to restore the condition to normal. Given the prevalence of PCOS and its impact on fertility, the use of noninvasive methods is of crucial significance. NAC can control and treat pathological parameters and help as a harmless drug in the treatment of women with PCOS. https://pagepressjournals.org/index.php/bam/article/view/10409Polycystic ovary syndrome (PCOS)metforminN acetylcysteinestereologypathology |
| spellingShingle | Bahare Rafiee Saied Karbalay-doust Seyed Mohammad Bagher Tabei Negar Azarpira Sanaz Alaee Parvin Lohrasbi Soghra Bahmanpour Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary European Journal of Translational Myology Polycystic ovary syndrome (PCOS) metformin N acetylcysteine stereology pathology |
| title | Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| title_full | Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| title_fullStr | Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| title_full_unstemmed | Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| title_short | Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| title_sort | effects of n acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary |
| topic | Polycystic ovary syndrome (PCOS) metformin N acetylcysteine stereology pathology |
| url | https://pagepressjournals.org/index.php/bam/article/view/10409 |
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