| Summary: | 【Abstract】Objective: To study the adherence,
proliferation and osteogenesis of mesenchymal stem cells
(MSCs) cultured on different HA/ZrO
2
composites.
Methods: The simplex and graded HA/ZrO
2
compo-sites were prepared using dry-laid method. The surface to-pography of the composites was observed by scanning
electron microscope (SEM). The MSCs were isolated from
rabbits and cultured on experimental groups (simplex HA/
ZrO
2
composite, graded HA/ZrO
2
composite, pure HA or
pure ZrO
2
coatings respectively) and control group (ordinary
culture plate). Then, we observed the adherence, prolifera-tion and osteogenesis of the MSCs, detected the cellular
alkaline phosphatase (ALP) activities, extracted total RNA
and detected the mRNA expression of collagen I, osteocalcin
and osteopontin using the reverse transcription and poly-merase chain reaction (RT-PCR) method.
Results: The SEM images confirmed that the surface
of the simplex HA/ZrO
2
composite was coverd by discon-tiguous HA layer with clear visualization of the partial ZrO
2
matrix, while the surface of the graded HA/ZrO
2
composite
was fairly rough with porosity. X-ray diffraction showed
that after high temperature sintering, the ZrO
2
phase still
remained, while the HA phase was transformed to β-Ca
3
(PO
4
)
2
, α-Ca
3
(PO
4
)
2
and CaZrO
3
phases on the surface of
both composites. Cell culture indicated that the HA/
ZrO
2
composites supported cell attachment. Neither ALP
expression nor mRNA expression of collagen I, osteocalcin
or osteopontin from RT-PCR results showed significant de-viation among four groups.
Conclusion: Among these four composites, the graded
HA/ZrO
2
composite promotes the MSCs proliferation and
the osteogenic differentiation to a certain extent.
Key words: Zirconium oxide; Durapatite; Mesenchy-mal stem cells
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