Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential
In this study, we proposed a three-dimensional (3D) printed porous (termed as 3DPP) scaffold composed of bioceramic (beta-tricalcium phosphate (β-TCP)) and thermoreversible biopolymer (pluronic F-127 (PF127)) that may provide bone tissue ingrowth and loading support for bone defect treatment. The in...
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| Language: | English |
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/5/1971 |
| _version_ | 1797474543237857280 |
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| author | Kuo-Sheng Hung May-Show Chen Wen-Chien Lan Yung-Chieh Cho Takashi Saito Bai-Hung Huang Hsin-Yu Tsai Chia-Chien Hsieh Keng-Liang Ou Hung-Yang Lin |
| author_facet | Kuo-Sheng Hung May-Show Chen Wen-Chien Lan Yung-Chieh Cho Takashi Saito Bai-Hung Huang Hsin-Yu Tsai Chia-Chien Hsieh Keng-Liang Ou Hung-Yang Lin |
| author_sort | Kuo-Sheng Hung |
| collection | DOAJ |
| description | In this study, we proposed a three-dimensional (3D) printed porous (termed as 3DPP) scaffold composed of bioceramic (beta-tricalcium phosphate (β-TCP)) and thermoreversible biopolymer (pluronic F-127 (PF127)) that may provide bone tissue ingrowth and loading support for bone defect treatment. The investigated scaffolds were printed in three different ranges of pore sizes for comparison (3DPP-1: 150–200 μm, 3DPP-2: 250–300 μm, and 3DPP-3: 300–350 μm). The material properties and biocompatibility of the 3DPP scaffolds were characterized using scanning electron microscopy, X-ray diffractometry, contact angle goniometry, compression testing, and cell viability assay. In addition, micro-computed tomography was applied to investigate bone regeneration behavior of the 3DPP scaffolds in the mini-pig model. Analytical results showed that the 3DPP scaffolds exhibited well-defined porosity, excellent microstructural interconnectivity, and acceptable wettability (θ < 90°). Among all groups, the 3DPP-1 possessed a significantly highest compressive force 273 ± 20.8 Kgf (* <i>p</i> < 0.05). In vitro experiment results also revealed good cell viability and cell attachment behavior in all 3DPP scaffolds. Furthermore, the 3DPP-3 scaffold showed a significantly higher percentage of bone formation volume than the 3DPP-1 scaffold at week 8 (* <i>p</i> < 0.05) and week 12 (* <i>p</i> < 0.05). Hence, the 3DPP scaffold composed of β-TCP and F-127 is a promising candidate to promote bone tissue ingrowth into the porous scaffold with decent biocompatibility. This scaffold particularly fabricated with a pore size of around 350 μm (i.e., 3DPP-3 scaffold) can provide proper loading support and promote bone regeneration in bone defects when applied in dental and orthopedic fields. |
| first_indexed | 2024-03-09T20:31:36Z |
| format | Article |
| id | doaj.art-edc2fb7571f14a049c385491a4318caa |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T20:31:36Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-edc2fb7571f14a049c385491a4318caa2023-11-23T23:21:49ZengMDPI AGMaterials1996-19442022-03-01155197110.3390/ma15051971Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration PotentialKuo-Sheng Hung0May-Show Chen1Wen-Chien Lan2Yung-Chieh Cho3Takashi Saito4Bai-Hung Huang5Hsin-Yu Tsai6Chia-Chien Hsieh7Keng-Liang Ou8Hung-Yang Lin9Graduate Institute of Injury Prevention and Control, College of Public Health, Taipei Medical University, Taipei 110, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Ishikari 061-0293, JapanBiomedical Technology R & D Center, China Medical University, Taichung 404, TaiwanDivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Ishikari 061-0293, JapanGraduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei 110, TaiwanBiomedical Technology R & D Center, China Medical University, Taichung 404, TaiwanDepartment of Dentistry, Fu Jen Catholic University Hospital, Fu Jen Catholic University, New Taipei City 242, TaiwanIn this study, we proposed a three-dimensional (3D) printed porous (termed as 3DPP) scaffold composed of bioceramic (beta-tricalcium phosphate (β-TCP)) and thermoreversible biopolymer (pluronic F-127 (PF127)) that may provide bone tissue ingrowth and loading support for bone defect treatment. The investigated scaffolds were printed in three different ranges of pore sizes for comparison (3DPP-1: 150–200 μm, 3DPP-2: 250–300 μm, and 3DPP-3: 300–350 μm). The material properties and biocompatibility of the 3DPP scaffolds were characterized using scanning electron microscopy, X-ray diffractometry, contact angle goniometry, compression testing, and cell viability assay. In addition, micro-computed tomography was applied to investigate bone regeneration behavior of the 3DPP scaffolds in the mini-pig model. Analytical results showed that the 3DPP scaffolds exhibited well-defined porosity, excellent microstructural interconnectivity, and acceptable wettability (θ < 90°). Among all groups, the 3DPP-1 possessed a significantly highest compressive force 273 ± 20.8 Kgf (* <i>p</i> < 0.05). In vitro experiment results also revealed good cell viability and cell attachment behavior in all 3DPP scaffolds. Furthermore, the 3DPP-3 scaffold showed a significantly higher percentage of bone formation volume than the 3DPP-1 scaffold at week 8 (* <i>p</i> < 0.05) and week 12 (* <i>p</i> < 0.05). Hence, the 3DPP scaffold composed of β-TCP and F-127 is a promising candidate to promote bone tissue ingrowth into the porous scaffold with decent biocompatibility. This scaffold particularly fabricated with a pore size of around 350 μm (i.e., 3DPP-3 scaffold) can provide proper loading support and promote bone regeneration in bone defects when applied in dental and orthopedic fields.https://www.mdpi.com/1996-1944/15/5/19713D printingbone regenerationbiocompatibilitytricalcium phosphatepluronic F127 |
| spellingShingle | Kuo-Sheng Hung May-Show Chen Wen-Chien Lan Yung-Chieh Cho Takashi Saito Bai-Hung Huang Hsin-Yu Tsai Chia-Chien Hsieh Keng-Liang Ou Hung-Yang Lin Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential Materials 3D printing bone regeneration biocompatibility tricalcium phosphate pluronic F127 |
| title | Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential |
| title_full | Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential |
| title_fullStr | Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential |
| title_full_unstemmed | Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential |
| title_short | Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential |
| title_sort | three dimensional printing of a hybrid bioceramic and biopolymer porous scaffold for promoting bone regeneration potential |
| topic | 3D printing bone regeneration biocompatibility tricalcium phosphate pluronic F127 |
| url | https://www.mdpi.com/1996-1944/15/5/1971 |
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