Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons
<p>Abstract</p> <p>Background</p> <p>Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of c...
| Main Authors: | , , , , |
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| Format: | Article |
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BMC
2011-11-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/11/244 |
| _version_ | 1818389865941696512 |
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| author | Siddiqui Huma Nederbragt Alexander J Lagesen Karin Jeansson Stig L Jakobsen Kjetill S |
| author_facet | Siddiqui Huma Nederbragt Alexander J Lagesen Karin Jeansson Stig L Jakobsen Kjetill S |
| author_sort | Siddiqui Huma |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques.</p> <p>Results</p> <p>Sequencing of the V1V2 and V6 regions of the 16S ribosomal RNA gene using the 454 GS FLX system was performed to characterize the possible bacterial composition in 8 culture-negative (<100,000 CFU/ml) healthy female urine specimens. Sequences were compared to 16S rRNA databases and showed significant diversity, with the predominant genera detected being <it>Lactobacillus</it>, <it>Prevotella </it>and <it>Gardnerella</it>. The bacterial profiles in the female urine samples studied were complex; considerable variation between individuals was observed and a common microbial signature was not evident. Notably, a significant amount of sequences belonging to bacteria with a known pathogenic potential was observed. The number of operational taxonomic units (OTUs) for individual samples varied substantially and was in the range of 20 - 500.</p> <p>Conclusions</p> <p>Normal female urine displays a noticeable and variable bacterial 16S rDNA sequence richness, which includes fastidious and anaerobic bacteria previously shown to be associated with female urogenital pathology.</p> |
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| format | Article |
| id | doaj.art-edc6d2be2f67400cbb510d2713d815e1 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T04:48:32Z |
| publishDate | 2011-11-01 |
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| series | BMC Microbiology |
| spelling | doaj.art-edc6d2be2f67400cbb510d2713d815e12022-12-21T23:16:37ZengBMCBMC Microbiology1471-21802011-11-0111124410.1186/1471-2180-11-244Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA ampliconsSiddiqui HumaNederbragt Alexander JLagesen KarinJeansson Stig LJakobsen Kjetill S<p>Abstract</p> <p>Background</p> <p>Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques.</p> <p>Results</p> <p>Sequencing of the V1V2 and V6 regions of the 16S ribosomal RNA gene using the 454 GS FLX system was performed to characterize the possible bacterial composition in 8 culture-negative (<100,000 CFU/ml) healthy female urine specimens. Sequences were compared to 16S rRNA databases and showed significant diversity, with the predominant genera detected being <it>Lactobacillus</it>, <it>Prevotella </it>and <it>Gardnerella</it>. The bacterial profiles in the female urine samples studied were complex; considerable variation between individuals was observed and a common microbial signature was not evident. Notably, a significant amount of sequences belonging to bacteria with a known pathogenic potential was observed. The number of operational taxonomic units (OTUs) for individual samples varied substantially and was in the range of 20 - 500.</p> <p>Conclusions</p> <p>Normal female urine displays a noticeable and variable bacterial 16S rDNA sequence richness, which includes fastidious and anaerobic bacteria previously shown to be associated with female urogenital pathology.</p>http://www.biomedcentral.com/1471-2180/11/244 |
| spellingShingle | Siddiqui Huma Nederbragt Alexander J Lagesen Karin Jeansson Stig L Jakobsen Kjetill S Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons BMC Microbiology |
| title | Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons |
| title_full | Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons |
| title_fullStr | Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons |
| title_full_unstemmed | Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons |
| title_short | Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons |
| title_sort | assessing diversity of the female urine microbiota by high throughput sequencing of 16s rdna amplicons |
| url | http://www.biomedcentral.com/1471-2180/11/244 |
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