Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants
<i>Agrobacterium</i>-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an <i>Agrobacterium</i>-based transient approach and the breakthrough discovery of CRISPR/...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10882 |
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| author | Maninder Kaur Pooja Manchanda Anu Kalia Farah K. Ahmed Eugenie Nepovimova Kamil Kuca Kamel A. Abd-Elsalam |
| author_facet | Maninder Kaur Pooja Manchanda Anu Kalia Farah K. Ahmed Eugenie Nepovimova Kamil Kuca Kamel A. Abd-Elsalam |
| author_sort | Maninder Kaur |
| collection | DOAJ |
| description | <i>Agrobacterium</i>-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an <i>Agrobacterium</i>-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on <i>Agrobacterium</i>-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future. |
| first_indexed | 2024-03-10T06:58:26Z |
| format | Article |
| id | doaj.art-edd4a037fed94b52854832420bae85be |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:58:26Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-edd4a037fed94b52854832420bae85be2023-11-22T16:16:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122191088210.3390/ijms221910882Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop PlantsManinder Kaur0Pooja Manchanda1Anu Kalia2Farah K. Ahmed3Eugenie Nepovimova4Kamil Kuca5Kamel A. Abd-Elsalam6School of Agricultural Biotechnology, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab 141004, IndiaSchool of Agricultural Biotechnology, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab 141004, IndiaElectron Microscopy and Nanoscience Laboratory, Department of Soil Science, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab 141004, IndiaBiotechnology English Program, Faculty of Agriculture, Cairo University, Giza 12613, EgyptDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech RepublicDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech RepublicPlant Pathology Research Institute, Agricultural Research Center (ARC), 9-Gamaa St., Giza 12619, Egypt<i>Agrobacterium</i>-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an <i>Agrobacterium</i>-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on <i>Agrobacterium</i>-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future.https://www.mdpi.com/1422-0067/22/19/10882<i>Agrobacterium</i>CRISPR/Cas9genome editingtargeted site modificationtransgene-freetransfer-DNA |
| spellingShingle | Maninder Kaur Pooja Manchanda Anu Kalia Farah K. Ahmed Eugenie Nepovimova Kamil Kuca Kamel A. Abd-Elsalam Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants International Journal of Molecular Sciences <i>Agrobacterium</i> CRISPR/Cas9 genome editing targeted site modification transgene-free transfer-DNA |
| title | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
| title_full | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
| title_fullStr | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
| title_full_unstemmed | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
| title_short | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
| title_sort | agroinfiltration mediated scalable transient gene expression in genome edited crop plants |
| topic | <i>Agrobacterium</i> CRISPR/Cas9 genome editing targeted site modification transgene-free transfer-DNA |
| url | https://www.mdpi.com/1422-0067/22/19/10882 |
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