Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique
To address which mitochondria-related nuclear differentially expressed genes (DEGs) and related pathways are altered during human oocyte maturation, single-cell analysis was performed in three oocyte states: in vivo matured (M-IVO), in vitro matured (M-IVT), and failed to mature in vitro (IM-IVT). T...
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2022-01-01
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| author | Zhi-Yong Yang Min Ye Ya-Xin Xing Qi-Gui Xie Jian-Hong Zhou Xin-Rui Qi Kehkooi Kee Ri-Cheng Chian |
| author_facet | Zhi-Yong Yang Min Ye Ya-Xin Xing Qi-Gui Xie Jian-Hong Zhou Xin-Rui Qi Kehkooi Kee Ri-Cheng Chian |
| author_sort | Zhi-Yong Yang |
| collection | DOAJ |
| description | To address which mitochondria-related nuclear differentially expressed genes (DEGs) and related pathways are altered during human oocyte maturation, single-cell analysis was performed in three oocyte states: in vivo matured (M-IVO), in vitro matured (M-IVT), and failed to mature in vitro (IM-IVT). There were 691 DEGs and 16 mitochondria-related DEGs in the comparison of M-IVT vs. IM-IVT oocytes, and 2281 DEGs and 160 mitochondria-related DEGs in the comparison of M-IVT vs. M-IVO oocytes, respectively. The GO and KEGG analyses showed that most of them were involved in pathways such as oxidative phosphorylation, pyruvate metabolism, peroxisome, and amino acid metabolism, i.e., valine, leucine, isoleucine, glycine, serine, and threonine metabolism or degradation. During the progress of oocyte maturation, the metabolic pathway, which derives the main source of ATP, shifted from glucose metabolism to pyruvate and fatty acid oxidation in order to maintain a low level of damaging reactive oxygen species (ROS) production. Although the immature oocytes could be cultured to a mature stage by an in vitro technique (IVM), there were still some differences in mitochondria-related regulations, which showed that the mitochondria were regulated by nuclear genes to compensate for their developmental needs. Meanwhile, the results indicated that the current IVM culture medium should be optimized to compensate for the special need for further development according to this disclosure, as it was a latent strategy to improve the effectiveness of the IVM procedure. |
| first_indexed | 2024-03-10T01:43:46Z |
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| language | English |
| last_indexed | 2024-03-10T01:43:46Z |
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| spelling | doaj.art-edd60ae9e9784ea4bf7c225e115fb8492023-11-23T13:19:19ZengMDPI AGCells2073-44092022-01-0111229710.3390/cells11020297Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM TechniqueZhi-Yong Yang0Min Ye1Ya-Xin Xing2Qi-Gui Xie3Jian-Hong Zhou4Xin-Rui Qi5Kehkooi Kee6Ri-Cheng Chian7Center for Reproductive Medicine, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaCenter for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaCenter for Reproductive Medicine, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Human Anatomy, Histology and Embryology, Tongji University School of Medicine, Shanghai 200092, ChinaCenter for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaCenter for Reproductive Medicine, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaTo address which mitochondria-related nuclear differentially expressed genes (DEGs) and related pathways are altered during human oocyte maturation, single-cell analysis was performed in three oocyte states: in vivo matured (M-IVO), in vitro matured (M-IVT), and failed to mature in vitro (IM-IVT). There were 691 DEGs and 16 mitochondria-related DEGs in the comparison of M-IVT vs. IM-IVT oocytes, and 2281 DEGs and 160 mitochondria-related DEGs in the comparison of M-IVT vs. M-IVO oocytes, respectively. The GO and KEGG analyses showed that most of them were involved in pathways such as oxidative phosphorylation, pyruvate metabolism, peroxisome, and amino acid metabolism, i.e., valine, leucine, isoleucine, glycine, serine, and threonine metabolism or degradation. During the progress of oocyte maturation, the metabolic pathway, which derives the main source of ATP, shifted from glucose metabolism to pyruvate and fatty acid oxidation in order to maintain a low level of damaging reactive oxygen species (ROS) production. Although the immature oocytes could be cultured to a mature stage by an in vitro technique (IVM), there were still some differences in mitochondria-related regulations, which showed that the mitochondria were regulated by nuclear genes to compensate for their developmental needs. Meanwhile, the results indicated that the current IVM culture medium should be optimized to compensate for the special need for further development according to this disclosure, as it was a latent strategy to improve the effectiveness of the IVM procedure.https://www.mdpi.com/2073-4409/11/2/297human oocytein vitro maturationIVMmitochondriagene expressionRNAseq |
| spellingShingle | Zhi-Yong Yang Min Ye Ya-Xin Xing Qi-Gui Xie Jian-Hong Zhou Xin-Rui Qi Kehkooi Kee Ri-Cheng Chian Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique Cells human oocyte in vitro maturation IVM mitochondria gene expression RNAseq |
| title | Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique |
| title_full | Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique |
| title_fullStr | Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique |
| title_full_unstemmed | Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique |
| title_short | Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique |
| title_sort | changes in the mitochondria related nuclear gene expression profile during human oocyte maturation by the ivm technique |
| topic | human oocyte in vitro maturation IVM mitochondria gene expression RNAseq |
| url | https://www.mdpi.com/2073-4409/11/2/297 |
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