The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri
Xanthomonas citri subsp. citri (Xcc) is the major causal agent of citrus canker disease. The XAC1347 gene, which encodes a conserved membrane protein in Xcc, is required for virulence during infection. However, the molecular events mediated by XAC1347 remain unclear. In this study, we reported that...
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01171/full |
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| author | Xiaojing Fan Jing Guo Yinghui Zhou Tao Zhuo Xun Hu Huasong Zou |
| author_facet | Xiaojing Fan Jing Guo Yinghui Zhou Tao Zhuo Xun Hu Huasong Zou |
| author_sort | Xiaojing Fan |
| collection | DOAJ |
| description | Xanthomonas citri subsp. citri (Xcc) is the major causal agent of citrus canker disease. The XAC1347 gene, which encodes a conserved membrane protein in Xcc, is required for virulence during infection. However, the molecular events mediated by XAC1347 remain unclear. In this study, we reported that XAC1347 gene is positively regulated by two component regulatory system ColRS and required for type III secretion system function. A non-polar deletion mutant of the XAC1347 gene resulted in a Hrp minus phenotype in plants and reduced copper homeostasis. Real-time PCR experiments indicated that XAC1347 gene is induced by copper ions. The expression levels of representative genes from four hrp operons, including hrpB1, hrcV, hrpF, and hrpD6, were reduced in XAC1347 mutant, indicating that XAC1347 is involved hrp gene expression. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T06:01:29Z |
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| spelling | doaj.art-edd712cb47494117b6461b7a27e744f82022-12-22T00:35:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01171328051The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citriXiaojing Fan0Jing Guo1Yinghui Zhou2Tao Zhuo3Xun Hu4Huasong Zou5State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian University Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian University Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian University Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian University Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian University Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, ChinaXanthomonas citri subsp. citri (Xcc) is the major causal agent of citrus canker disease. The XAC1347 gene, which encodes a conserved membrane protein in Xcc, is required for virulence during infection. However, the molecular events mediated by XAC1347 remain unclear. In this study, we reported that XAC1347 gene is positively regulated by two component regulatory system ColRS and required for type III secretion system function. A non-polar deletion mutant of the XAC1347 gene resulted in a Hrp minus phenotype in plants and reduced copper homeostasis. Real-time PCR experiments indicated that XAC1347 gene is induced by copper ions. The expression levels of representative genes from four hrp operons, including hrpB1, hrcV, hrpF, and hrpD6, were reduced in XAC1347 mutant, indicating that XAC1347 is involved hrp gene expression.https://www.frontiersin.org/article/10.3389/fmicb.2018.01171/fullXanthomonas citri subsp. citriXAC1347membrane proteinhrp generegulation |
| spellingShingle | Xiaojing Fan Jing Guo Yinghui Zhou Tao Zhuo Xun Hu Huasong Zou The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri Frontiers in Microbiology Xanthomonas citri subsp. citri XAC1347 membrane protein hrp gene regulation |
| title | The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri |
| title_full | The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri |
| title_fullStr | The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri |
| title_full_unstemmed | The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri |
| title_short | The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri |
| title_sort | colrs regulated membrane protein gene xac1347 is involved in copper homeostasis and hrp gene expression in xanthomonas citri subsp citri |
| topic | Xanthomonas citri subsp. citri XAC1347 membrane protein hrp gene regulation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01171/full |
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