Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials
Zingiber mioga is a highly economic crop that is used to produce vegetables, spices and herbal pharmaceuticals. Its edible flower bud contributes most to the economic value, but the big leaves were discarded as agricultural waste, which urgently needs to be exploited. In this work, polysaccharides f...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417723004303 |
| _version_ | 1797386810103431168 |
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| author | Jingchun Yang Shuaiyi Dong Xu Zhou Wen Zhang Yunzhu Gu Lixue Zheng Guihong Yang Jing Wang Yang Zhang |
| author_facet | Jingchun Yang Shuaiyi Dong Xu Zhou Wen Zhang Yunzhu Gu Lixue Zheng Guihong Yang Jing Wang Yang Zhang |
| author_sort | Jingchun Yang |
| collection | DOAJ |
| description | Zingiber mioga is a highly economic crop that is used to produce vegetables, spices and herbal pharmaceuticals. Its edible flower bud contributes most to the economic value, but the big leaves were discarded as agricultural waste, which urgently needs to be exploited. In this work, polysaccharides from waste Z. mioga leaves (PWZMLs) were extracted using ultrasonic-microwave-assisted extraction (UMAE). After purification and characterization, the antioxidation and anticoagulation of PWZMLs were evaluated to appraise the potential in cardiovascular protection. Under the liquid–solid ratio of 26: 1 mL/g, after ultrasonication at 495 W for 10 min, followed by microwaving at 490 W for 5 min, the yield of PWZMLs achieved to 6.22 ± 0.14 %, notably higher (P < 0.01) than other methods, and ultrasound contributed more to the yield than microwave. Various analyses confirmed that PWZMLs were negatively charged polysaccharides with galacturonic acid the dominant uronic acid. PWZMLs exerted excellent antioxidant capacity, especially for scavenging 1, 1-diphenyl-2-picrylhydrazyl radical. PWZMLs also elicited promising anticoagulant property, particularly for prolonging activated partial thromboplastin time and lowering fibrinogen, which were almost equivalent to heparin at the same concentration. PWZMLs contained two polysaccharide fractions (199.53 and 275.42 kDa) that could synergistically contribute to the pronounced antioxidant and anticoagulant activities. The PWZMLs extracted with optimized UMAE have great potential in cardiovascular protection. |
| first_indexed | 2024-03-08T22:15:49Z |
| format | Article |
| id | doaj.art-eddd862983724ada9a29bbd208fcf159 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-08T22:15:49Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-eddd862983724ada9a29bbd208fcf1592023-12-19T04:16:47ZengElsevierUltrasonics Sonochemistry1350-41772023-12-01101106718Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentialsJingchun Yang0Shuaiyi Dong1Xu Zhou2Wen Zhang3Yunzhu Gu4Lixue Zheng5Guihong Yang6Jing Wang7Yang Zhang8School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaSchool of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaCorresponding author.; School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, ChinaZingiber mioga is a highly economic crop that is used to produce vegetables, spices and herbal pharmaceuticals. Its edible flower bud contributes most to the economic value, but the big leaves were discarded as agricultural waste, which urgently needs to be exploited. In this work, polysaccharides from waste Z. mioga leaves (PWZMLs) were extracted using ultrasonic-microwave-assisted extraction (UMAE). After purification and characterization, the antioxidation and anticoagulation of PWZMLs were evaluated to appraise the potential in cardiovascular protection. Under the liquid–solid ratio of 26: 1 mL/g, after ultrasonication at 495 W for 10 min, followed by microwaving at 490 W for 5 min, the yield of PWZMLs achieved to 6.22 ± 0.14 %, notably higher (P < 0.01) than other methods, and ultrasound contributed more to the yield than microwave. Various analyses confirmed that PWZMLs were negatively charged polysaccharides with galacturonic acid the dominant uronic acid. PWZMLs exerted excellent antioxidant capacity, especially for scavenging 1, 1-diphenyl-2-picrylhydrazyl radical. PWZMLs also elicited promising anticoagulant property, particularly for prolonging activated partial thromboplastin time and lowering fibrinogen, which were almost equivalent to heparin at the same concentration. PWZMLs contained two polysaccharide fractions (199.53 and 275.42 kDa) that could synergistically contribute to the pronounced antioxidant and anticoagulant activities. The PWZMLs extracted with optimized UMAE have great potential in cardiovascular protection.http://www.sciencedirect.com/science/article/pii/S1350417723004303Waste Zingiber mioga leavesPolysaccharidesUltrasonic-microwave-assisted extractionAntioxidationAnticoagulation |
| spellingShingle | Jingchun Yang Shuaiyi Dong Xu Zhou Wen Zhang Yunzhu Gu Lixue Zheng Guihong Yang Jing Wang Yang Zhang Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials Ultrasonics Sonochemistry Waste Zingiber mioga leaves Polysaccharides Ultrasonic-microwave-assisted extraction Antioxidation Anticoagulation |
| title | Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials |
| title_full | Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials |
| title_fullStr | Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials |
| title_full_unstemmed | Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials |
| title_short | Polysaccharides from waste Zingiber mioga leaves: Ultrasonic-microwave-assisted extraction, characterization, antioxidant and anticoagulant potentials |
| title_sort | polysaccharides from waste zingiber mioga leaves ultrasonic microwave assisted extraction characterization antioxidant and anticoagulant potentials |
| topic | Waste Zingiber mioga leaves Polysaccharides Ultrasonic-microwave-assisted extraction Antioxidation Anticoagulation |
| url | http://www.sciencedirect.com/science/article/pii/S1350417723004303 |
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