The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at t...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2359 |
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| author | Anna Wawrzyk Michał Łobacz Agnieszka Adamczuk Weronika Sofińska-Chmiel Sławomir Wilczyński Mansur Rahnama |
| author_facet | Anna Wawrzyk Michał Łobacz Agnieszka Adamczuk Weronika Sofińska-Chmiel Sławomir Wilczyński Mansur Rahnama |
| author_sort | Anna Wawrzyk |
| collection | DOAJ |
| description | The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material. |
| first_indexed | 2024-03-10T05:15:17Z |
| format | Article |
| id | doaj.art-eddfefa734404cf7aac57fb7e110c86e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:17Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-eddfefa734404cf7aac57fb7e110c86e2023-11-23T00:30:15ZengMDPI AGMicroorganisms2076-26072021-11-01911235910.3390/microorganisms9112359The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental ImplantsAnna Wawrzyk0Michał Łobacz1Agnieszka Adamczuk2Weronika Sofińska-Chmiel3Sławomir Wilczyński4Mansur Rahnama5Silesian Park of Medical Technology Kardio-Med Silesia in Zabrze, M. Curie Skłodowskiej 10C Str., 41-800 Zabrze, PolandChair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6 Str., 20-093 Lublin, PolandInstitute of Agrophysics PAS, Doświadczalna 4 Str., 20-290 Lublin, PolandAnalytical Laboratory, Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie Skłodowska University, Maria Curie Skłodowska Sq. 2, 20-031 Lublin, PolandDepartament of Basic Medical Science, Faculty of Farmaceutical Sciences in Sosnowiec, Medical University of Silesia, Kasztanowa Str. 3, 41-200 Sosnowiec, PolandChair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6 Str., 20-093 Lublin, PolandThe aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material.https://www.mdpi.com/2076-2607/9/11/2359diode laserimplant-prostheticsuperstructuremicroorganismsporcelainzirconia |
| spellingShingle | Anna Wawrzyk Michał Łobacz Agnieszka Adamczuk Weronika Sofińska-Chmiel Sławomir Wilczyński Mansur Rahnama The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants Microorganisms diode laser implant-prosthetic superstructure microorganisms porcelain zirconia |
| title | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
| title_full | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
| title_fullStr | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
| title_full_unstemmed | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
| title_short | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
| title_sort | use of a diode laser for removal of microorganisms from the surfaces of zirconia and porcelain applied to superstructure dental implants |
| topic | diode laser implant-prosthetic superstructure microorganisms porcelain zirconia |
| url | https://www.mdpi.com/2076-2607/9/11/2359 |
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