Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice
Passive smoking is extensively studied because of its harmfulness to human health. In this study, the effects of fermented green tea waste extract gels (GTEG) on oxidative damage in mice exposed to short-term cigarette smoke (CS) were investigated. The GTEG is prepared from green tea waste extract a...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/8/461 |
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| author | Jiangwen Liu Yijun Wang Lei Sun Dongfeng Guo Xuefeng Wu Dongdong Mu Xingjiang Li |
| author_facet | Jiangwen Liu Yijun Wang Lei Sun Dongfeng Guo Xuefeng Wu Dongdong Mu Xingjiang Li |
| author_sort | Jiangwen Liu |
| collection | DOAJ |
| description | Passive smoking is extensively studied because of its harmfulness to human health. In this study, the effects of fermented green tea waste extract gels (GTEG) on oxidative damage in mice exposed to short-term cigarette smoke (CS) were investigated. The GTEG is prepared from green tea waste extract and microbial transglutaminase (MTGase). The lung injury model of mice was established through passive smoking for 5 days. The experimental results revealed the following findings. (1) The GTEG induced by MTGase has obvious gel properties; (2) GTEG has strong biological activity and antioxidant properties in vitro; (3) The passive smoking model was established successfully; specifically, the lung tissue of the model mice exhibited inflammatory symptoms, oxidative stress response appeared in their bodies, and their inflammatory indicators increased; (4) Compared with the passive smoking model group, the mice, which were exposed to CS and received GTEG treatment, exhibited increased food intake and body weight; increased total superoxide dismutase and glutathione peroxidase activity in serum; significant decreases (<i>p</i> < 0.05) in the content levels of the inflammatory factors malondialdehyde, interleukin (IL)-6, and tumor necrosis factor α (TNF-α); and inhibited expression of IL-6, IL-33, TNF-α, and IL-1β inflammatory genes. The results indicated that taking GTEG can relieve the oxidative stress injury of mice caused by short-term CS and has antioxidant properties. |
| first_indexed | 2024-03-09T04:25:22Z |
| format | Article |
| id | doaj.art-ede0878d282b471e913842180fff3345 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T04:25:22Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-ede0878d282b471e913842180fff33452023-12-03T13:42:00ZengMDPI AGGels2310-28612022-07-018846110.3390/gels8080461Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking MiceJiangwen Liu0Yijun Wang1Lei Sun2Dongfeng Guo3Xuefeng Wu4Dongdong Mu5Xingjiang Li6School of Food and Bioengineering, Hefei University of Technology, Hefei 230009, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, ChinaSinopharm Health Biotechnology (Huangshan) Co., Ltd., Huangshan 245061, ChinaTechnology Center of Anhui China Tobacco Industry Co., Ltd., Hefei 230088, ChinaSchool of Food and Bioengineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Bioengineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Bioengineering, Hefei University of Technology, Hefei 230009, ChinaPassive smoking is extensively studied because of its harmfulness to human health. In this study, the effects of fermented green tea waste extract gels (GTEG) on oxidative damage in mice exposed to short-term cigarette smoke (CS) were investigated. The GTEG is prepared from green tea waste extract and microbial transglutaminase (MTGase). The lung injury model of mice was established through passive smoking for 5 days. The experimental results revealed the following findings. (1) The GTEG induced by MTGase has obvious gel properties; (2) GTEG has strong biological activity and antioxidant properties in vitro; (3) The passive smoking model was established successfully; specifically, the lung tissue of the model mice exhibited inflammatory symptoms, oxidative stress response appeared in their bodies, and their inflammatory indicators increased; (4) Compared with the passive smoking model group, the mice, which were exposed to CS and received GTEG treatment, exhibited increased food intake and body weight; increased total superoxide dismutase and glutathione peroxidase activity in serum; significant decreases (<i>p</i> < 0.05) in the content levels of the inflammatory factors malondialdehyde, interleukin (IL)-6, and tumor necrosis factor α (TNF-α); and inhibited expression of IL-6, IL-33, TNF-α, and IL-1β inflammatory genes. The results indicated that taking GTEG can relieve the oxidative stress injury of mice caused by short-term CS and has antioxidant properties.https://www.mdpi.com/2310-2861/8/8/461fermented green tea waste extract gelsgel propertiesoxidative damageshort-termpassive smoking |
| spellingShingle | Jiangwen Liu Yijun Wang Lei Sun Dongfeng Guo Xuefeng Wu Dongdong Mu Xingjiang Li Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice Gels fermented green tea waste extract gels gel properties oxidative damage short-term passive smoking |
| title | Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice |
| title_full | Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice |
| title_fullStr | Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice |
| title_full_unstemmed | Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice |
| title_short | Effects of Fermented Green Tea Waste Extract Gels on Oxidative Damage in Short-Term Passive Smoking Mice |
| title_sort | effects of fermented green tea waste extract gels on oxidative damage in short term passive smoking mice |
| topic | fermented green tea waste extract gels gel properties oxidative damage short-term passive smoking |
| url | https://www.mdpi.com/2310-2861/8/8/461 |
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