Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival
<p>Abstract</p> <p>Background</p> <p>Increased expression of Eph receptor tyrosine kinases and their ephrin ligands has been implicated in tumor progression in a number of malignancies. This report describes aberrant expression of these genes in ovarian cancer, the comm...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-06-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/6/144 |
| _version_ | 1818646446411350016 |
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| author | Lincoln Douglas Duffy Shannon Cummings Margaret Newton Tanya Sabesan Sabe Spanevello Mark D Herath Nirmitha I Boyle Glen Parsons Peter G Boyd Andrew W |
| author_facet | Lincoln Douglas Duffy Shannon Cummings Margaret Newton Tanya Sabesan Sabe Spanevello Mark D Herath Nirmitha I Boyle Glen Parsons Peter G Boyd Andrew W |
| author_sort | Lincoln Douglas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Increased expression of Eph receptor tyrosine kinases and their ephrin ligands has been implicated in tumor progression in a number of malignancies. This report describes aberrant expression of these genes in ovarian cancer, the commonest cause of death amongst gynaecological malignancies.</p> <p>Methods</p> <p>Eph and ephrin expression was determined using quantitative real time RT-PCR. Correlation of gene expression was measured using Spearman's rho statistic. Survival was analysed using log-rank analysis and (was visualised by) Kaplan-Meier survival curves.</p> <p>Results</p> <p>Greater than 10 fold over-expression of EphA1 and a more modest over-expression of EphA2 were observed in partially overlapping subsets of tumors. Over-expression of EphA1 strongly correlated (r = 0.801; p < 0.01) with the high affinity ligand ephrin A1. A similar trend was observed between EphA2 and ephrin A1 (r = 0.387; p = 0.06). A striking correlation of both ephrin A1 and ephrin A5 expression with poor survival (r = -0.470; p = 0.02 and r = -0.562; p < 0.01) was observed. Intriguingly, there was no correlation between survival and other clinical parameters or Eph expression.</p> <p>Conclusion</p> <p>These data imply that increased levels of ephrins A1 and A5 in the presence of high expression of Ephs A1 and A2 lead to a more aggressive tumor phenotype. The known functions of Eph/ephrin signalling in cell de-adhesion and movement may explain the observed correlation of ephrin expression with poor prognosis.</p> |
| first_indexed | 2024-12-17T00:46:46Z |
| format | Article |
| id | doaj.art-ede0ae9ba9594aac9f9be84c053c162f |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-17T00:46:46Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-ede0ae9ba9594aac9f9be84c053c162f2022-12-21T22:09:54ZengBMCBMC Cancer1471-24072006-06-016114410.1186/1471-2407-6-144Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survivalLincoln DouglasDuffy ShannonCummings MargaretNewton TanyaSabesan SabeSpanevello Mark DHerath Nirmitha IBoyle GlenParsons Peter GBoyd Andrew W<p>Abstract</p> <p>Background</p> <p>Increased expression of Eph receptor tyrosine kinases and their ephrin ligands has been implicated in tumor progression in a number of malignancies. This report describes aberrant expression of these genes in ovarian cancer, the commonest cause of death amongst gynaecological malignancies.</p> <p>Methods</p> <p>Eph and ephrin expression was determined using quantitative real time RT-PCR. Correlation of gene expression was measured using Spearman's rho statistic. Survival was analysed using log-rank analysis and (was visualised by) Kaplan-Meier survival curves.</p> <p>Results</p> <p>Greater than 10 fold over-expression of EphA1 and a more modest over-expression of EphA2 were observed in partially overlapping subsets of tumors. Over-expression of EphA1 strongly correlated (r = 0.801; p < 0.01) with the high affinity ligand ephrin A1. A similar trend was observed between EphA2 and ephrin A1 (r = 0.387; p = 0.06). A striking correlation of both ephrin A1 and ephrin A5 expression with poor survival (r = -0.470; p = 0.02 and r = -0.562; p < 0.01) was observed. Intriguingly, there was no correlation between survival and other clinical parameters or Eph expression.</p> <p>Conclusion</p> <p>These data imply that increased levels of ephrins A1 and A5 in the presence of high expression of Ephs A1 and A2 lead to a more aggressive tumor phenotype. The known functions of Eph/ephrin signalling in cell de-adhesion and movement may explain the observed correlation of ephrin expression with poor prognosis.</p>http://www.biomedcentral.com/1471-2407/6/144 |
| spellingShingle | Lincoln Douglas Duffy Shannon Cummings Margaret Newton Tanya Sabesan Sabe Spanevello Mark D Herath Nirmitha I Boyle Glen Parsons Peter G Boyd Andrew W Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival BMC Cancer |
| title | Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival |
| title_full | Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival |
| title_fullStr | Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival |
| title_full_unstemmed | Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival |
| title_short | Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival |
| title_sort | over expression of eph and ephrin genes in advanced ovarian cancer ephrin gene expression correlates with shortened survival |
| url | http://www.biomedcentral.com/1471-2407/6/144 |
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