Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutr...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4128779?pdf=render |
| _version_ | 1818493359318106112 |
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| author | Li Liu Ling Chen Ying Shao Lili Zhang Tilman Floehr Hongxia Xiao Yan Yan Kathrin Eichbaum Henner Hollert Lingling Wu |
| author_facet | Li Liu Ling Chen Ying Shao Lili Zhang Tilman Floehr Hongxia Xiao Yan Yan Kathrin Eichbaum Henner Hollert Lingling Wu |
| author_sort | Li Liu |
| collection | DOAJ |
| description | In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants. |
| first_indexed | 2024-12-10T17:54:12Z |
| format | Article |
| id | doaj.art-ede2ef03c3b54132a9197efd0cb88579 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T17:54:12Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ede2ef03c3b54132a9197efd0cb885792022-12-22T01:38:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10474810.1371/journal.pone.0104748Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.Li LiuLing ChenYing ShaoLili ZhangTilman FloehrHongxia XiaoYan YanKathrin EichbaumHenner HollertLingling WuIn this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.http://europepmc.org/articles/PMC4128779?pdf=render |
| spellingShingle | Li Liu Ling Chen Ying Shao Lili Zhang Tilman Floehr Hongxia Xiao Yan Yan Kathrin Eichbaum Henner Hollert Lingling Wu Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. PLoS ONE |
| title | Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. |
| title_full | Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. |
| title_fullStr | Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. |
| title_full_unstemmed | Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. |
| title_short | Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities. |
| title_sort | evaluation of the ecotoxicity of sediments from yangtze river estuary and contribution of priority pahs to ah receptor mediated activities |
| url | http://europepmc.org/articles/PMC4128779?pdf=render |
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