Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells
DNA methylation, repressive histone modifications, and PIWI-interacting RNAs are essential for controlling retroelement silencing in mammalian germ lines. Dysregulation of retroelement silencing is associated with male sterility. Although retroelement silencing mechanisms have been extensively studi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/76822 |
| _version_ | 1811235824951361536 |
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| author | Kei Fukuda Yoshinori Makino Satoru Kaneko Chikako Shimura Yuki Okada Kenji Ichiyanagi Yoichi Shinkai |
| author_facet | Kei Fukuda Yoshinori Makino Satoru Kaneko Chikako Shimura Yuki Okada Kenji Ichiyanagi Yoichi Shinkai |
| author_sort | Kei Fukuda |
| collection | DOAJ |
| description | DNA methylation, repressive histone modifications, and PIWI-interacting RNAs are essential for controlling retroelement silencing in mammalian germ lines. Dysregulation of retroelement silencing is associated with male sterility. Although retroelement silencing mechanisms have been extensively studied in mouse germ cells, little progress has been made in humans. Here, we show that the Krüppel-associated box domain zinc finger proteins are associated with DNA methylation of retroelements in human primordial germ cells. Further, we show that the hominoid-specific retroelement SINE-VNTR-Alus (SVA) is subjected to transcription-directed de novo DNA methylation during human spermatogenesis. The degree of de novo DNA methylation in SVAs varies among human individuals, which confers significant inter-individual epigenetic variation in sperm. Collectively, our results highlight potential molecular mechanisms for the regulation of retroelements in human male germ cells. |
| first_indexed | 2024-04-12T11:58:38Z |
| format | Article |
| id | doaj.art-ede302e6533b4adfa10cd77f596ff144 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T11:58:38Z |
| publishDate | 2022-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ede302e6533b4adfa10cd77f596ff1442022-12-22T03:33:56ZengeLife Sciences Publications LtdeLife2050-084X2022-03-011110.7554/eLife.76822Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cellsKei Fukuda0https://orcid.org/0000-0002-4111-9409Yoshinori Makino1Satoru Kaneko2Chikako Shimura3Yuki Okada4Kenji Ichiyanagi5Yoichi Shinkai6https://orcid.org/0000-0002-6051-2484Cellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako, JapanLaboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, JapanDepartment of Obstetrics and Gynecology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, JapanCellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako, JapanLaboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, JapanLaboratory of Genome and Epigenome Dynamics, Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, JapanCellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako, JapanDNA methylation, repressive histone modifications, and PIWI-interacting RNAs are essential for controlling retroelement silencing in mammalian germ lines. Dysregulation of retroelement silencing is associated with male sterility. Although retroelement silencing mechanisms have been extensively studied in mouse germ cells, little progress has been made in humans. Here, we show that the Krüppel-associated box domain zinc finger proteins are associated with DNA methylation of retroelements in human primordial germ cells. Further, we show that the hominoid-specific retroelement SINE-VNTR-Alus (SVA) is subjected to transcription-directed de novo DNA methylation during human spermatogenesis. The degree of de novo DNA methylation in SVAs varies among human individuals, which confers significant inter-individual epigenetic variation in sperm. Collectively, our results highlight potential molecular mechanisms for the regulation of retroelements in human male germ cells.https://elifesciences.org/articles/76822retroelementsDNA methylationhuman male germ cells |
| spellingShingle | Kei Fukuda Yoshinori Makino Satoru Kaneko Chikako Shimura Yuki Okada Kenji Ichiyanagi Yoichi Shinkai Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells eLife retroelements DNA methylation human male germ cells |
| title | Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells |
| title_full | Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells |
| title_fullStr | Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells |
| title_full_unstemmed | Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells |
| title_short | Potential role of KRAB-ZFP binding and transcriptional states on DNA methylation of retroelements in human male germ cells |
| title_sort | potential role of krab zfp binding and transcriptional states on dna methylation of retroelements in human male germ cells |
| topic | retroelements DNA methylation human male germ cells |
| url | https://elifesciences.org/articles/76822 |
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