Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies
<i>Drosophila melanogaster (Drosophila)</i> models of cancer are emerging as powerful tools to investigate the basic mechanisms underlying tumour progression and identify novel therapeutics. Rapid and inexpensive, it is possible to carry out genetic and drug screens at a far larger scale...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/10/5101 |
| _version_ | 1797534486277128192 |
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| author | Jamie Adams Andreu Casali Kyra Campbell |
| author_facet | Jamie Adams Andreu Casali Kyra Campbell |
| author_sort | Jamie Adams |
| collection | DOAJ |
| description | <i>Drosophila melanogaster (Drosophila)</i> models of cancer are emerging as powerful tools to investigate the basic mechanisms underlying tumour progression and identify novel therapeutics. Rapid and inexpensive, it is possible to carry out genetic and drug screens at a far larger scale than in vertebrate organisms. Such whole-organism-based drug screens permits assessment of drug absorption and toxicity, reducing the possibility of false positives. Activating mutations in the Wnt and Ras signalling pathways are common in many epithelial cancers, and when driven in the adult <i>Drosophila</i> midgut, it induces aggressive intestinal tumour-like outgrowths that recapitulate many aspects of human colorectal cancer (CRC). Here we have taken a <i>Drosophila</i> CRC model in which tumourous cells are marked with both GFP and luciferase reporter genes, and developed novel high-throughput assays for quantifying tumour burden. Leveraging these assays, we find that the <i>Drosophila</i> CRC model responds rapidly to treatment with standard CRC-drugs, opening the door to future rapid genetic and drug screens. |
| first_indexed | 2024-03-10T11:31:11Z |
| format | Article |
| id | doaj.art-ede4b34d0a2b48bfa2cf77a6271c0eb1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:31:11Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ede4b34d0a2b48bfa2cf77a6271c0eb12023-11-21T19:18:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012210510110.3390/ijms22105101Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard ChemotherapiesJamie Adams0Andreu Casali1Kyra Campbell2Department of Biomedical Science and Bateson Centre, The University of Sheffield, Sheffield S10 2TN, UKDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida and IRBLleida, 25198 Lleida, SpainDepartment of Biomedical Science and Bateson Centre, The University of Sheffield, Sheffield S10 2TN, UK<i>Drosophila melanogaster (Drosophila)</i> models of cancer are emerging as powerful tools to investigate the basic mechanisms underlying tumour progression and identify novel therapeutics. Rapid and inexpensive, it is possible to carry out genetic and drug screens at a far larger scale than in vertebrate organisms. Such whole-organism-based drug screens permits assessment of drug absorption and toxicity, reducing the possibility of false positives. Activating mutations in the Wnt and Ras signalling pathways are common in many epithelial cancers, and when driven in the adult <i>Drosophila</i> midgut, it induces aggressive intestinal tumour-like outgrowths that recapitulate many aspects of human colorectal cancer (CRC). Here we have taken a <i>Drosophila</i> CRC model in which tumourous cells are marked with both GFP and luciferase reporter genes, and developed novel high-throughput assays for quantifying tumour burden. Leveraging these assays, we find that the <i>Drosophila</i> CRC model responds rapidly to treatment with standard CRC-drugs, opening the door to future rapid genetic and drug screens.https://www.mdpi.com/1422-0067/22/10/5101<i>Drosophila</i>colorectal cancerhigh-throughputdrug screening |
| spellingShingle | Jamie Adams Andreu Casali Kyra Campbell Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies International Journal of Molecular Sciences <i>Drosophila</i> colorectal cancer high-throughput drug screening |
| title | Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies |
| title_full | Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies |
| title_fullStr | Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies |
| title_full_unstemmed | Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies |
| title_short | Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a <i>Drosophila melanogaster</i> Model of Colorectal Cancer to Standard Chemotherapies |
| title_sort | sensitive high throughput assays for tumour burden reveal the response of a i drosophila melanogaster i model of colorectal cancer to standard chemotherapies |
| topic | <i>Drosophila</i> colorectal cancer high-throughput drug screening |
| url | https://www.mdpi.com/1422-0067/22/10/5101 |
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