Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia
Relapsing fever (RF) is an arthropod-borne disease caused by <i>Borrelia</i> spirochete, which is one of the major public health concerns in endemic regions including Africa. However, information on <i>Borrelia spirochetes</i> is limited in Zambia. Here, we investigate the &l...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/1/200 |
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| author | Yongjin Qiu Herman M. Chambaro Kozue Sato David Squarre Edgar Simulundu Masahiro Kajihara Katendi Changula Manyando Simbotwe Hayato Harima Joseph Ndebe Ladslav Moonga Ryo Nakao Ayato Takada Bernard Mudenda Hang’ombe Hirofumi Sawa Hiroki Kawabata |
| author_facet | Yongjin Qiu Herman M. Chambaro Kozue Sato David Squarre Edgar Simulundu Masahiro Kajihara Katendi Changula Manyando Simbotwe Hayato Harima Joseph Ndebe Ladslav Moonga Ryo Nakao Ayato Takada Bernard Mudenda Hang’ombe Hirofumi Sawa Hiroki Kawabata |
| author_sort | Yongjin Qiu |
| collection | DOAJ |
| description | Relapsing fever (RF) is an arthropod-borne disease caused by <i>Borrelia</i> spirochete, which is one of the major public health concerns in endemic regions including Africa. However, information on <i>Borrelia spirochetes</i> is limited in Zambia. Here, we investigate the <i>Borrelia spirochetes</i> harbored by <i>Ornithodoros</i> ticks in Zambian National Parks. We analyzed 182 DNA samples pooled from 886 <i>Ornithodoros</i> ticks. Of these, 43 tested positive, and their sequence revealed that the ticks harbored both Old and New World RF borreliae. This research presents the first evidence of Old-World RF borreliae in Zambia. The New World RF borreliae detected herein differed from the <i>Candidatus</i> Borrelia fainii previously reported in Zambia and were closely related to the pathogenic <i>Borrelia</i> sp. VS4 identified in Tanzania. Additionally, <i>Borrelia theileri</i> was recently reported in Zambia. Hence, at least four different <i>Borrelia</i> species occur in Zambia, and the organisms causing relapsing fever there might be more complex than previously thought. We empirically confirmed that real-time PCR with TaqMan minor groove binder probes accurately and simultaneously detected both Old and New World RF. In this manner, they could facilitate quantitative analyses of both types of RF borreliae. Subsequent investigations should endeavor to isolate the aforementioned <i>Borrelia</i> spp. and perform serosurveys on patients with RF. |
| first_indexed | 2024-03-09T11:38:42Z |
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| id | doaj.art-ede5fc914885452292e84ccb31bbbfe8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:38:42Z |
| publishDate | 2023-01-01 |
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| series | Microorganisms |
| spelling | doaj.art-ede5fc914885452292e84ccb31bbbfe82023-11-30T23:37:52ZengMDPI AGMicroorganisms2076-26072023-01-0111120010.3390/microorganisms11010200Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in ZambiaYongjin Qiu0Herman M. Chambaro1Kozue Sato2David Squarre3Edgar Simulundu4Masahiro Kajihara5Katendi Changula6Manyando Simbotwe7Hayato Harima8Joseph Ndebe9Ladslav Moonga10Ryo Nakao11Ayato Takada12Bernard Mudenda Hang’ombe13Hirofumi Sawa14Hiroki Kawabata15Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo 001-0020, JapanDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo 001-0020, JapanDepartment of Bacteriology-I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640, JapanFQM Trident Wildlife and Forestry Programs, P.O. Box 230022, Kalumbila 10101, ZambiaDepartment of Diseases Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDivision of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo 001-0020, JapanDepartment of Paraclinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDepartment of Diseases Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDivision of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo 001-0020, JapanDepartment of Diseases Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDepartment of Paraclinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaLaboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Kita-ku, Sapporo 060-0818, JapanDepartment of Diseases Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDepartment of Paraclinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, ZambiaDivision of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo 001-0020, JapanDepartment of Bacteriology-I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640, JapanRelapsing fever (RF) is an arthropod-borne disease caused by <i>Borrelia</i> spirochete, which is one of the major public health concerns in endemic regions including Africa. However, information on <i>Borrelia spirochetes</i> is limited in Zambia. Here, we investigate the <i>Borrelia spirochetes</i> harbored by <i>Ornithodoros</i> ticks in Zambian National Parks. We analyzed 182 DNA samples pooled from 886 <i>Ornithodoros</i> ticks. Of these, 43 tested positive, and their sequence revealed that the ticks harbored both Old and New World RF borreliae. This research presents the first evidence of Old-World RF borreliae in Zambia. The New World RF borreliae detected herein differed from the <i>Candidatus</i> Borrelia fainii previously reported in Zambia and were closely related to the pathogenic <i>Borrelia</i> sp. VS4 identified in Tanzania. Additionally, <i>Borrelia theileri</i> was recently reported in Zambia. Hence, at least four different <i>Borrelia</i> species occur in Zambia, and the organisms causing relapsing fever there might be more complex than previously thought. We empirically confirmed that real-time PCR with TaqMan minor groove binder probes accurately and simultaneously detected both Old and New World RF. In this manner, they could facilitate quantitative analyses of both types of RF borreliae. Subsequent investigations should endeavor to isolate the aforementioned <i>Borrelia</i> spp. and perform serosurveys on patients with RF.https://www.mdpi.com/2076-2607/11/1/200<i>Borrelia</i>national park<i>Ornithodoros moubata</i><i>Ornithodoros porcinus</i>relapsing feverTaqMan MGB |
| spellingShingle | Yongjin Qiu Herman M. Chambaro Kozue Sato David Squarre Edgar Simulundu Masahiro Kajihara Katendi Changula Manyando Simbotwe Hayato Harima Joseph Ndebe Ladslav Moonga Ryo Nakao Ayato Takada Bernard Mudenda Hang’ombe Hirofumi Sawa Hiroki Kawabata Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia Microorganisms <i>Borrelia</i> national park <i>Ornithodoros moubata</i> <i>Ornithodoros porcinus</i> relapsing fever TaqMan MGB |
| title | Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia |
| title_full | Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia |
| title_fullStr | Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia |
| title_full_unstemmed | Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia |
| title_short | Detection of Old and New World Relapsing Fever Borreliae in <i>Ornithodoros</i> Ticks Collected from Warthog Burrows in Zambia |
| title_sort | detection of old and new world relapsing fever borreliae in i ornithodoros i ticks collected from warthog burrows in zambia |
| topic | <i>Borrelia</i> national park <i>Ornithodoros moubata</i> <i>Ornithodoros porcinus</i> relapsing fever TaqMan MGB |
| url | https://www.mdpi.com/2076-2607/11/1/200 |
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