Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators
Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene express...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00547/full |
| _version_ | 1811223347651936256 |
|---|---|
| author | Jun Wang Jun Wang Run-Ze Sun Run-Ze Sun Guo Cheng Guo Cheng Qiang Li Qiang Li Yan-Nan He Yu Wang Yu Wang Yi-Bin Lan Yi-Bin Lan Si-Yu Li Si-Yu Li Yan-Rong Zhu Wen-Feng Song Xue Zhang Xiao-Di Cui Wu Chen |
| author_facet | Jun Wang Jun Wang Run-Ze Sun Run-Ze Sun Guo Cheng Guo Cheng Qiang Li Qiang Li Yan-Nan He Yu Wang Yu Wang Yi-Bin Lan Yi-Bin Lan Si-Yu Li Si-Yu Li Yan-Rong Zhu Wen-Feng Song Xue Zhang Xiao-Di Cui Wu Chen |
| author_sort | Jun Wang |
| collection | DOAJ |
| description | Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries. |
| first_indexed | 2024-04-12T08:31:21Z |
| format | Article |
| id | doaj.art-ede60210b3b4427c860d8a0aa5c79e85 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T08:31:21Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ede60210b3b4427c860d8a0aa5c79e852022-12-22T03:40:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00547246188Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal RegulatorsJun Wang0Jun Wang1Run-Ze Sun2Run-Ze Sun3Guo Cheng4Guo Cheng5Qiang Li6Qiang Li7Yan-Nan He8Yu Wang9Yu Wang10Yi-Bin Lan11Yi-Bin Lan12Si-Yu Li13Si-Yu Li14Yan-Rong Zhu15Wen-Feng Song16Xue Zhang17Xiao-Di Cui18Wu Chen19Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of AgricultureBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaKey Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of ScienceBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaGrape and Wine Research Institute, Guangxi Academy of Agricultural SciencesNanning, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Enology, Northwest A&F UniversityYangling, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of AgricultureBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of AgricultureBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of AgricultureBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural UniversityBeijing, ChinaCITIC Guoan Wine Co. Ltd.Xinjiang, ChinaLight environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries.http://journal.frontiersin.org/article/10.3389/fpls.2017.00547/fullVitis viniferasunlight exposurephenolic compoundstranscriptometranscription factorphytohormone signaling |
| spellingShingle | Jun Wang Jun Wang Run-Ze Sun Run-Ze Sun Guo Cheng Guo Cheng Qiang Li Qiang Li Yan-Nan He Yu Wang Yu Wang Yi-Bin Lan Yi-Bin Lan Si-Yu Li Si-Yu Li Yan-Rong Zhu Wen-Feng Song Xue Zhang Xiao-Di Cui Wu Chen Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators Frontiers in Plant Science Vitis vinifera sunlight exposure phenolic compounds transcriptome transcription factor phytohormone signaling |
| title | Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators |
| title_full | Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators |
| title_fullStr | Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators |
| title_full_unstemmed | Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators |
| title_short | Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators |
| title_sort | light induced variation in phenolic compounds in cabernet sauvignon grapes vitis vinifera l involves extensive transcriptome reprogramming of biosynthetic enzymes transcription factors and phytohormonal regulators |
| topic | Vitis vinifera sunlight exposure phenolic compounds transcriptome transcription factor phytohormone signaling |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00547/full |
| work_keys_str_mv | AT junwang lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT junwang lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT runzesun lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT runzesun lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT guocheng lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT guocheng lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT qiangli lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT qiangli lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yannanhe lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yuwang lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yuwang lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yibinlan lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yibinlan lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT siyuli lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT siyuli lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT yanrongzhu lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT wenfengsong lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT xuezhang lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT xiaodicui lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators AT wuchen lightinducedvariationinphenoliccompoundsincabernetsauvignongrapesvitisviniferalinvolvesextensivetranscriptomereprogrammingofbiosyntheticenzymestranscriptionfactorsandphytohormonalregulators |