GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
<p>Abstract</p> <p>Background</p> <p>Overexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. We aimed to investigate the clinical significance of GOLPH3 expression...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/168 |
| _version_ | 1819135693332414464 |
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| author | Li Huan Guo Ling Chen Shu-Wei Zhao Xiao-Hui Zhuang Shi-Min Wang Li-Ping Song Li-Bing Song Ming |
| author_facet | Li Huan Guo Ling Chen Shu-Wei Zhao Xiao-Hui Zhuang Shi-Min Wang Li-Ping Song Li-Bing Song Ming |
| author_sort | Li Huan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Overexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. We aimed to investigate the clinical significance of GOLPH3 expression in the development and progression of clinically N0 (cN0) oral tongue cancer.</p> <p>Methods</p> <p>Real-time PCR and Western blotting analyses were employed to examine GOLPH3 expression in four oral tongue cancer cell lines, primary cultured normal tongue epithelial cells (TEC), eight matched pairs of oral tongue cancer samples and adjacent noncancerous tissue samples from the same patient. Immunohistochemistry (IHC) was performed to examine GOLPH3 protein expression in paraffin-embedded tissues from 179 cN0 oral tongue cancer patients. Statistical analyses were applied to evaluate the diagnostic value and the associations of GOLPH3 expression with clinical parameters.</p> <p>Results</p> <p>GOLPH3 mRNA and protein was up-regulated in oral tongue cancer cell lines and cancerous tissues compared with that in primary cultured normal tongue epithelial cells (TEC) and adjacent noncancerous tissue samples. GOLPH3 protein level was positively correlated with clinical stage (<it>P</it> = 0.001), T classification (<it>P</it> = 0.001), N classification (<it>P</it> = 0.043) and recurrence (<it>P</it> = 0.009). Patients with higher GOLPH3 expression had shorter overall survival time, whereas those with lower GOLPH3 expression had longer survival time.</p> <p>Conclusion</p> <p>Our results suggest GOLPH3 overexpression is associated with poor prognosis for cN0 oral tongue cancer patients and may represent a novel and useful prognostic indicator for cN0 oral tongue cancer.</p> |
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| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-22T10:23:08Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
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| series | Journal of Translational Medicine |
| spelling | doaj.art-ede712084fc04cc081fd3937efbb020d2022-12-21T18:29:35ZengBMCJournal of Translational Medicine1479-58762012-08-0110116810.1186/1479-5876-10-168GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancerLi HuanGuo LingChen Shu-WeiZhao Xiao-HuiZhuang Shi-MinWang Li-PingSong Li-BingSong Ming<p>Abstract</p> <p>Background</p> <p>Overexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. We aimed to investigate the clinical significance of GOLPH3 expression in the development and progression of clinically N0 (cN0) oral tongue cancer.</p> <p>Methods</p> <p>Real-time PCR and Western blotting analyses were employed to examine GOLPH3 expression in four oral tongue cancer cell lines, primary cultured normal tongue epithelial cells (TEC), eight matched pairs of oral tongue cancer samples and adjacent noncancerous tissue samples from the same patient. Immunohistochemistry (IHC) was performed to examine GOLPH3 protein expression in paraffin-embedded tissues from 179 cN0 oral tongue cancer patients. Statistical analyses were applied to evaluate the diagnostic value and the associations of GOLPH3 expression with clinical parameters.</p> <p>Results</p> <p>GOLPH3 mRNA and protein was up-regulated in oral tongue cancer cell lines and cancerous tissues compared with that in primary cultured normal tongue epithelial cells (TEC) and adjacent noncancerous tissue samples. GOLPH3 protein level was positively correlated with clinical stage (<it>P</it> = 0.001), T classification (<it>P</it> = 0.001), N classification (<it>P</it> = 0.043) and recurrence (<it>P</it> = 0.009). Patients with higher GOLPH3 expression had shorter overall survival time, whereas those with lower GOLPH3 expression had longer survival time.</p> <p>Conclusion</p> <p>Our results suggest GOLPH3 overexpression is associated with poor prognosis for cN0 oral tongue cancer patients and may represent a novel and useful prognostic indicator for cN0 oral tongue cancer.</p>http://www.translational-medicine.com/content/10/1/168GOLPH3PrognosiscN0 oral tongue cancer |
| spellingShingle | Li Huan Guo Ling Chen Shu-Wei Zhao Xiao-Hui Zhuang Shi-Min Wang Li-Ping Song Li-Bing Song Ming GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer Journal of Translational Medicine GOLPH3 Prognosis cN0 oral tongue cancer |
| title | GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer |
| title_full | GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer |
| title_fullStr | GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer |
| title_full_unstemmed | GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer |
| title_short | GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer |
| title_sort | golph3 overexpression correlates with tumor progression and poor prognosis in patients with clinically n0 oral tongue cancer |
| topic | GOLPH3 Prognosis cN0 oral tongue cancer |
| url | http://www.translational-medicine.com/content/10/1/168 |
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