Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i>
The coprophilous ascomycete <i>Podospora anserina</i> is known to have a high potential to synthesize a wide array of secondary metabolites (SMs). However, to date, the characterization of SMs in this species, as in other filamentous fungal species, is far less than expected by the funct...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/9 |
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| author | Ling Shen Catherine Roullier François-Hugues Porée Thomas Gaslonde Ludivine Riffault-Valois Olivier Grovel Gwenaël Ruprich-Robert Florence Chapeland-Leclerc |
| author_facet | Ling Shen Catherine Roullier François-Hugues Porée Thomas Gaslonde Ludivine Riffault-Valois Olivier Grovel Gwenaël Ruprich-Robert Florence Chapeland-Leclerc |
| author_sort | Ling Shen |
| collection | DOAJ |
| description | The coprophilous ascomycete <i>Podospora anserina</i> is known to have a high potential to synthesize a wide array of secondary metabolites (SMs). However, to date, the characterization of SMs in this species, as in other filamentous fungal species, is far less than expected by the functional prediction through genome mining, likely due to the inactivity of most SMs biosynthesis gene clusters (BGCs) under standard conditions. In this work, our main objective was to compare the global strategies usually used to deregulate SM gene clusters in <i>P. anserina</i>, including the variation of culture conditions and the modification of the chromatin state either by genetic manipulation or by chemical treatment, and to show the complementarity of the approaches between them. In this way, we showed that the metabolomics-driven comparative analysis unveils the unexpected diversity of metabolic changes in <i>P. anserina</i> and that the integrated strategies have a mutual complementary effect on the expression of the fungal metabolome. Then, our results demonstrate that metabolite production is significantly influenced by varied cultivation states and epigenetic modifications. We believe that the strategy described in this study will facilitate the discovery of fungal metabolites of interest and will improve the ability to prioritize the production of specific fungal SMs with an optimized treatment. |
| first_indexed | 2024-03-09T12:05:13Z |
| format | Article |
| id | doaj.art-edef326360094626bd0098865a2d1908 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:05:13Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-edef326360094626bd0098865a2d19082023-11-30T22:59:06ZengMDPI AGJournal of Fungi2309-608X2022-12-0191910.3390/jof9010009Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i>Ling Shen0Catherine Roullier1François-Hugues Porée2Thomas Gaslonde3Ludivine Riffault-Valois4Olivier Grovel5Gwenaël Ruprich-Robert6Florence Chapeland-Leclerc7School of Life Science, Jiangsu Normal University, Xuzhou 221116, ChinaInstitut des Substances et Organismes de la Mer (ISOMER), UR 2160, Nantes Université, F-44000 Nantes, FranceLaboratoire de Chimie Thérapeutique, UMR 6226, ISCR, CNRS, Faculté de Pharmacie, Université de Rennes 1, F-35065 Rennes, FranceCibles Thérapeutiques et Conception de Médicaments (CiTCoM), UMR 8038, CNRS, Faculté de Pharmacie de Paris, Université Paris Cité, F-75006 Paris, FranceInstitut Pluridisciplinaire Hubert Curien, UMR 7178, CNRS, Université de Strasbourg, F-67000 Strasbourg, FranceInstitut des Substances et Organismes de la Mer (ISOMER), UR 2160, Nantes Université, F-44000 Nantes, FranceLaboratoire Interdisciplinaire des Energies de Demain (LIED), UMR 8236, CNRS, Université Paris Cité, F-75013 Paris, FranceLaboratoire Interdisciplinaire des Energies de Demain (LIED), UMR 8236, CNRS, Université Paris Cité, F-75013 Paris, FranceThe coprophilous ascomycete <i>Podospora anserina</i> is known to have a high potential to synthesize a wide array of secondary metabolites (SMs). However, to date, the characterization of SMs in this species, as in other filamentous fungal species, is far less than expected by the functional prediction through genome mining, likely due to the inactivity of most SMs biosynthesis gene clusters (BGCs) under standard conditions. In this work, our main objective was to compare the global strategies usually used to deregulate SM gene clusters in <i>P. anserina</i>, including the variation of culture conditions and the modification of the chromatin state either by genetic manipulation or by chemical treatment, and to show the complementarity of the approaches between them. In this way, we showed that the metabolomics-driven comparative analysis unveils the unexpected diversity of metabolic changes in <i>P. anserina</i> and that the integrated strategies have a mutual complementary effect on the expression of the fungal metabolome. Then, our results demonstrate that metabolite production is significantly influenced by varied cultivation states and epigenetic modifications. We believe that the strategy described in this study will facilitate the discovery of fungal metabolites of interest and will improve the ability to prioritize the production of specific fungal SMs with an optimized treatment.https://www.mdpi.com/2309-608X/9/1/9<i>Podospora anserina</i>secondary metabolitescomplementary strategiesmetabolomics |
| spellingShingle | Ling Shen Catherine Roullier François-Hugues Porée Thomas Gaslonde Ludivine Riffault-Valois Olivier Grovel Gwenaël Ruprich-Robert Florence Chapeland-Leclerc Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> Journal of Fungi <i>Podospora anserina</i> secondary metabolites complementary strategies metabolomics |
| title | Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> |
| title_full | Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> |
| title_fullStr | Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> |
| title_full_unstemmed | Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> |
| title_short | Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous Fungus <i>Podospora anserina</i> |
| title_sort | complementary strategies to unlock biosynthesis gene clusters encoding secondary metabolites in the filamentous fungus i podospora anserina i |
| topic | <i>Podospora anserina</i> secondary metabolites complementary strategies metabolomics |
| url | https://www.mdpi.com/2309-608X/9/1/9 |
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