Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma
Sarcomas are cancers that arise in soft tissues or bone and make up a small percentage of malignancies. In an effort to identify potential genetic targets for therapy, this study explores the genomic landscape of a metastatic undifferentiated pleomorphic sarcoma (UPS) with spindle cell morphology. T...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-03-01
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| Series: | Rare Tumors |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/rt/article/view/4603 |
| _version_ | 1818539397311627264 |
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| author | Paul Kurywchak Jeff Kiefer Elizabeth Lenkiewicz Lisa Evers Tara Holley Michael Barrett Glen J. Weiss |
| author_facet | Paul Kurywchak Jeff Kiefer Elizabeth Lenkiewicz Lisa Evers Tara Holley Michael Barrett Glen J. Weiss |
| author_sort | Paul Kurywchak |
| collection | DOAJ |
| description | Sarcomas are cancers that arise in soft tissues or bone and make up a small percentage of malignancies. In an effort to identify potential genetic targets for therapy, this study explores the genomic landscape of a metastatic undifferentiated pleomorphic sarcoma (UPS) with spindle cell morphology. Thick sections (50 μm) of formalin-fixed, paraffin-embedded tissue from a primary, recurrent, and metastatic tumor were collected and processed from a single patient for DNA content-based flow-sorting and analyses. Nuclei of diploid and aneuploid populations were sorted from the malignant tissues and their genomes interrogated with array comparative genomic hybridization. The third sample was highly degraded and did not contain any intact ploidy peaks in our flow assays. A 2.5N aneuploid population was identified in the primary and recurrent sample. We detected a series of shared and unique genomic aberrations in the sorted aneuploid populations. The patterns of aberrations suggest that two similar but independent clonal populations arose during the clinical history of this rare tumor. None of these aberrations were detected in the matching sorted diploid samples. The targeted regions of interest might play a role in UPS and may lead to clinical significance with further investigation. |
| first_indexed | 2024-12-11T21:41:29Z |
| format | Article |
| id | doaj.art-edf0b7701ac749f9bb9d89021f199552 |
| institution | Directory Open Access Journal |
| issn | 2036-3605 2036-3613 |
| language | English |
| last_indexed | 2024-12-11T21:41:29Z |
| publishDate | 2013-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Rare Tumors |
| spelling | doaj.art-edf0b7701ac749f9bb9d89021f1995522022-12-22T00:49:49ZengSAGE PublishingRare Tumors2036-36052036-36132013-03-0151e14e1410.4081/rt.2013.e142449Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcomaPaul Kurywchak0Jeff Kiefer1Elizabeth Lenkiewicz2Lisa Evers3Tara Holley4Michael Barrett5Glen J. Weiss6Cancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZCancer and Cell Biology Division, Translational Genomics Research Institute, Phoenix, AZ; Virginia G. Piper Cancer Center Clinical Trials at Scottsdale Healthcare, Scottsdale, AZSarcomas are cancers that arise in soft tissues or bone and make up a small percentage of malignancies. In an effort to identify potential genetic targets for therapy, this study explores the genomic landscape of a metastatic undifferentiated pleomorphic sarcoma (UPS) with spindle cell morphology. Thick sections (50 μm) of formalin-fixed, paraffin-embedded tissue from a primary, recurrent, and metastatic tumor were collected and processed from a single patient for DNA content-based flow-sorting and analyses. Nuclei of diploid and aneuploid populations were sorted from the malignant tissues and their genomes interrogated with array comparative genomic hybridization. The third sample was highly degraded and did not contain any intact ploidy peaks in our flow assays. A 2.5N aneuploid population was identified in the primary and recurrent sample. We detected a series of shared and unique genomic aberrations in the sorted aneuploid populations. The patterns of aberrations suggest that two similar but independent clonal populations arose during the clinical history of this rare tumor. None of these aberrations were detected in the matching sorted diploid samples. The targeted regions of interest might play a role in UPS and may lead to clinical significance with further investigation.http://www.pagepress.org/journals/index.php/rt/article/view/4603undifferentiated pleomorphic sarcoma, flow-sort cytometry, array comparative genomic hybridization |
| spellingShingle | Paul Kurywchak Jeff Kiefer Elizabeth Lenkiewicz Lisa Evers Tara Holley Michael Barrett Glen J. Weiss Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma Rare Tumors undifferentiated pleomorphic sarcoma, flow-sort cytometry, array comparative genomic hybridization |
| title | Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| title_full | Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| title_fullStr | Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| title_full_unstemmed | Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| title_short | Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| title_sort | elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma |
| topic | undifferentiated pleomorphic sarcoma, flow-sort cytometry, array comparative genomic hybridization |
| url | http://www.pagepress.org/journals/index.php/rt/article/view/4603 |
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