Deadpan contributes to the robustness of the notch response.
Notch signaling regulates many fundamental events including lateral inhibition and boundary formation to generate very reproducible patterns in developing tissues. Its targets include genes of the bHLH hairy and Enhancer of split [E(spl)] family, which contribute to many of these developmental decis...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3782438?pdf=render |
| _version_ | 1819163431498940416 |
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| author | A Burcu Babaoğlan Ben E Housden Marc Furriols Sarah J Bray |
| author_facet | A Burcu Babaoğlan Ben E Housden Marc Furriols Sarah J Bray |
| author_sort | A Burcu Babaoğlan |
| collection | DOAJ |
| description | Notch signaling regulates many fundamental events including lateral inhibition and boundary formation to generate very reproducible patterns in developing tissues. Its targets include genes of the bHLH hairy and Enhancer of split [E(spl)] family, which contribute to many of these developmental decisions. One member of this family in Drosophila, deadpan (dpn), was originally found to have functions independent of Notch in promoting neural development. Employing genome-wide chromatin-immunoprecipitation we have identified several Notch responsive enhancers in dpn, demonstrating its direct regulation by Notch in a range of contexts including the Drosophila wing and eye. dpn expression largely overlaps that of several E(spl) genes and the combined knock-down leads to more severe phenotypes than either alone. In addition, Dpn contributes to the establishment of Cut expression at the wing dorsal-ventral (D/V) boundary; in its absence Cut expression is delayed. Furthermore, over-expression of Dpn inhibits expression from E(spl) gene enhancers, but not vice versa, suggesting that dpn contributes to a feed-back mechanism that limits E(spl) gene expression following Notch activation. Thus the combined actions of dpn and E(spl) appear to provide a mechanism that confers an initial rapid output from Notch activity which becomes self-limited via feedback between the targets. |
| first_indexed | 2024-12-22T17:44:01Z |
| format | Article |
| id | doaj.art-edf22bdd87d5456bab21a31763f9066a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:44:01Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-edf22bdd87d5456bab21a31763f9066a2022-12-21T18:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7563210.1371/journal.pone.0075632Deadpan contributes to the robustness of the notch response.A Burcu BabaoğlanBen E HousdenMarc FurriolsSarah J BrayNotch signaling regulates many fundamental events including lateral inhibition and boundary formation to generate very reproducible patterns in developing tissues. Its targets include genes of the bHLH hairy and Enhancer of split [E(spl)] family, which contribute to many of these developmental decisions. One member of this family in Drosophila, deadpan (dpn), was originally found to have functions independent of Notch in promoting neural development. Employing genome-wide chromatin-immunoprecipitation we have identified several Notch responsive enhancers in dpn, demonstrating its direct regulation by Notch in a range of contexts including the Drosophila wing and eye. dpn expression largely overlaps that of several E(spl) genes and the combined knock-down leads to more severe phenotypes than either alone. In addition, Dpn contributes to the establishment of Cut expression at the wing dorsal-ventral (D/V) boundary; in its absence Cut expression is delayed. Furthermore, over-expression of Dpn inhibits expression from E(spl) gene enhancers, but not vice versa, suggesting that dpn contributes to a feed-back mechanism that limits E(spl) gene expression following Notch activation. Thus the combined actions of dpn and E(spl) appear to provide a mechanism that confers an initial rapid output from Notch activity which becomes self-limited via feedback between the targets.http://europepmc.org/articles/PMC3782438?pdf=render |
| spellingShingle | A Burcu Babaoğlan Ben E Housden Marc Furriols Sarah J Bray Deadpan contributes to the robustness of the notch response. PLoS ONE |
| title | Deadpan contributes to the robustness of the notch response. |
| title_full | Deadpan contributes to the robustness of the notch response. |
| title_fullStr | Deadpan contributes to the robustness of the notch response. |
| title_full_unstemmed | Deadpan contributes to the robustness of the notch response. |
| title_short | Deadpan contributes to the robustness of the notch response. |
| title_sort | deadpan contributes to the robustness of the notch response |
| url | http://europepmc.org/articles/PMC3782438?pdf=render |
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