Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein
Disease resistance (R) genes have been isolated from many plant species. Most encode nucleotide binding leucine-rich repeat (NLR) proteins that trigger a rapid localized programmed cell death called the hypersensitive response (HR) upon pathogen recognition. Despite their structural similarities, di...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-15-0014-R |
| _version_ | 1811290242237333504 |
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| author | Guan-Feng Wang Peter J. Balint-Kurti |
| author_facet | Guan-Feng Wang Peter J. Balint-Kurti |
| author_sort | Guan-Feng Wang |
| collection | DOAJ |
| description | Disease resistance (R) genes have been isolated from many plant species. Most encode nucleotide binding leucine-rich repeat (NLR) proteins that trigger a rapid localized programmed cell death called the hypersensitive response (HR) upon pathogen recognition. Despite their structural similarities, different NLR are distributed in a range of subcellular locations, and analogous domains play diverse functional roles. The autoactive maize NLR gene Rp1-D21 derives from an intragenic recombination between two NLR genes, Rp1-D and Rp1-dp2, and confers a HR independent of the presence of a pathogen. Rp1-D21 and its N-terminal coiled coil (CC) domain (CCD21) confer autoactive HR when transiently expressed in Nicotiana benthamiana. Rp1-D21 was predominantly localized in cytoplasm with a small amount in the nucleus, while CCD21 was localized in both nucleus and cytoplasm. Targeting of Rp1-D21 or CCD21 predominantly to either the nucleus or the cytoplasm abolished HR-inducing activity. Coexpression of Rp1-D21 or CCD21 constructs confined, respectively, to the nucleus and cytoplasm did not rescue full activity, suggesting nucleocytoplasmic movement was important for HR induction. This work emphasizes the diverse structural and subcellular localization requirements for activity found among plant NLR R genes. |
| first_indexed | 2024-04-13T04:08:19Z |
| format | Article |
| id | doaj.art-edf59342f65e4a9895156ce9528446e1 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:08:19Z |
| publishDate | 2015-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-edf59342f65e4a9895156ce9528446e12022-12-22T03:03:10ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-09-012891023103110.1094/MPMI-01-15-0014-RCytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 ProteinGuan-Feng Wang0Peter J. Balint-Kurti1Dept. of Plant Pathology, North Carolina State University, Raleigh, NC 27695, U.S.A.;Dept. of Plant Pathology, North Carolina State University, Raleigh, NC 27695, U.S.A.;Disease resistance (R) genes have been isolated from many plant species. Most encode nucleotide binding leucine-rich repeat (NLR) proteins that trigger a rapid localized programmed cell death called the hypersensitive response (HR) upon pathogen recognition. Despite their structural similarities, different NLR are distributed in a range of subcellular locations, and analogous domains play diverse functional roles. The autoactive maize NLR gene Rp1-D21 derives from an intragenic recombination between two NLR genes, Rp1-D and Rp1-dp2, and confers a HR independent of the presence of a pathogen. Rp1-D21 and its N-terminal coiled coil (CC) domain (CCD21) confer autoactive HR when transiently expressed in Nicotiana benthamiana. Rp1-D21 was predominantly localized in cytoplasm with a small amount in the nucleus, while CCD21 was localized in both nucleus and cytoplasm. Targeting of Rp1-D21 or CCD21 predominantly to either the nucleus or the cytoplasm abolished HR-inducing activity. Coexpression of Rp1-D21 or CCD21 constructs confined, respectively, to the nucleus and cytoplasm did not rescue full activity, suggesting nucleocytoplasmic movement was important for HR induction. This work emphasizes the diverse structural and subcellular localization requirements for activity found among plant NLR R genes.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-15-0014-R |
| spellingShingle | Guan-Feng Wang Peter J. Balint-Kurti Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein Molecular Plant-Microbe Interactions |
| title | Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein |
| title_full | Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein |
| title_fullStr | Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein |
| title_full_unstemmed | Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein |
| title_short | Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein |
| title_sort | cytoplasmic and nuclear localizations are important for the hypersensitive response conferred by maize autoactive rp1 d21 protein |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-15-0014-R |
| work_keys_str_mv | AT guanfengwang cytoplasmicandnuclearlocalizationsareimportantforthehypersensitiveresponseconferredbymaizeautoactiverp1d21protein AT peterjbalintkurti cytoplasmicandnuclearlocalizationsareimportantforthehypersensitiveresponseconferredbymaizeautoactiverp1d21protein |