The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease
Parkinsons disease is an important medico-social problem worldwide, with a lot of attention paid to preclinical studies to assess the efficacy of new treatments, including cell therapy. Study objective. To assess the migratory ability of multipotent mesenchymal stromal cells (MMSC) using differen...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Research Center of Neurology
2019-06-01
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| Series: | Анналы клинической и экспериментальной неврологии |
| Subjects: | |
| Online Access: | https://annaly-nevrologii.com/journal/pathID/article/viewFile/589/472 |
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| author | Marina M. Zafranskaya Daria B. Nizhegorodova Natalia E. Aleynikova Tatiana E. Kuznetsova Margarita I. Vanslav Tatiana V. Ignatovich Alexander V. Boiko Vladimir V. Ponomarev |
| author_facet | Marina M. Zafranskaya Daria B. Nizhegorodova Natalia E. Aleynikova Tatiana E. Kuznetsova Margarita I. Vanslav Tatiana V. Ignatovich Alexander V. Boiko Vladimir V. Ponomarev |
| author_sort | Marina M. Zafranskaya |
| collection | DOAJ |
| description | Parkinsons disease is an important medico-social problem worldwide, with a lot of attention paid to preclinical studies to assess the efficacy of new treatments, including cell therapy.
Study objective. To assess the migratory ability of multipotent mesenchymal stromal cells (MMSC) using different methods of administration in an experimental model of Parkinsons disease in laboratory rats.
Materials and methods. MMSC, stained with the PKH26 fluorescent dye, were systemically (intravenously) or locally (intranasally and intrathecally) administered to experimental animals with rotenone-induced Parkinsons disease. The migratory ability of MMSC was assessed on days 1 and 21 after administration, using immunofluorescence microscopy.
Results. The migratory ability of MMSC after both systemic and local administration was more pronounced in the animal group with the experimental model of Parkinsons disease compared with the control group. It was characterized by maximum accumulation of cells in the brain on the first day after administration, with viability preserved in the area of neuronal inflammation throughout 21 days.
Conclusion. Local administration (intranasal and intrathecal) leads to faster accumulation of MMSC in the brain of both the animals with the experimental model of Parkinsons disease and healthy rats. Intravenous administration of cell cultures also helps to reveal the migratory properties of MMSC and can form the basis for planning further studies of cell therapy in Parkinsons disease. |
| first_indexed | 2024-04-13T18:05:05Z |
| format | Article |
| id | doaj.art-edf5c13a32494ea5973a063fa0bc8916 |
| institution | Directory Open Access Journal |
| issn | 2075-5473 2409-2533 |
| language | English |
| last_indexed | 2024-04-13T18:05:05Z |
| publishDate | 2019-06-01 |
| publisher | Research Center of Neurology |
| record_format | Article |
| series | Анналы клинической и экспериментальной неврологии |
| spelling | doaj.art-edf5c13a32494ea5973a063fa0bc89162022-12-22T02:36:06ZengResearch Center of NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-25332019-06-01132324010.25692/ACEN.2019.2.4453The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s diseaseMarina M. Zafranskaya0Daria B. Nizhegorodova1Natalia E. Aleynikova2Tatiana E. Kuznetsova3Margarita I. Vanslav4Tatiana V. Ignatovich5Alexander V. Boiko6Vladimir V. Ponomarev7Belarusian Medical Academy of Postgraduate EducationBelarusian Medical Academy of Postgraduate EducationBelarusian Medical Academy of Postgraduate EducationInternational Sakharov Environmental Institute of the Belarusian State UniversityBelarusian Medical Academy of Postgraduate EducationBelarusian Medical Academy of Postgraduate EducationBelarusian Medical Academy of Postgraduate EducationBelarusian Medical Academy of Postgraduate EducationParkinsons disease is an important medico-social problem worldwide, with a lot of attention paid to preclinical studies to assess the efficacy of new treatments, including cell therapy. Study objective. To assess the migratory ability of multipotent mesenchymal stromal cells (MMSC) using different methods of administration in an experimental model of Parkinsons disease in laboratory rats. Materials and methods. MMSC, stained with the PKH26 fluorescent dye, were systemically (intravenously) or locally (intranasally and intrathecally) administered to experimental animals with rotenone-induced Parkinsons disease. The migratory ability of MMSC was assessed on days 1 and 21 after administration, using immunofluorescence microscopy. Results. The migratory ability of MMSC after both systemic and local administration was more pronounced in the animal group with the experimental model of Parkinsons disease compared with the control group. It was characterized by maximum accumulation of cells in the brain on the first day after administration, with viability preserved in the area of neuronal inflammation throughout 21 days. Conclusion. Local administration (intranasal and intrathecal) leads to faster accumulation of MMSC in the brain of both the animals with the experimental model of Parkinsons disease and healthy rats. Intravenous administration of cell cultures also helps to reveal the migratory properties of MMSC and can form the basis for planning further studies of cell therapy in Parkinsons disease.https://annaly-nevrologii.com/journal/pathID/article/viewFile/589/472multipotent mesenchymal stromal cellsparkinson’s diseaseexperimental model of parkinson’s diseasemigrationcell therapy |
| spellingShingle | Marina M. Zafranskaya Daria B. Nizhegorodova Natalia E. Aleynikova Tatiana E. Kuznetsova Margarita I. Vanslav Tatiana V. Ignatovich Alexander V. Boiko Vladimir V. Ponomarev The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease Анналы клинической и экспериментальной неврологии multipotent mesenchymal stromal cells parkinson’s disease experimental model of parkinson’s disease migration cell therapy |
| title | The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease |
| title_full | The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease |
| title_fullStr | The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease |
| title_full_unstemmed | The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease |
| title_short | The migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of Parkinson’s disease |
| title_sort | migration of multipotent mesenchymal stromal cells after systemic and local administration in an experimental model of parkinson s disease |
| topic | multipotent mesenchymal stromal cells parkinson’s disease experimental model of parkinson’s disease migration cell therapy |
| url | https://annaly-nevrologii.com/journal/pathID/article/viewFile/589/472 |
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