Marker-free co-selection for successive rounds of prime editing in human cells
Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. Here the authors develop a co-selection strategy to facilitate prime editing in human cells and provide design principles to prevent the formation of undesir...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33669-z |
| _version_ | 1811226700377227264 |
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| author | Sébastien Levesque Diana Mayorga Jean-Philippe Fiset Claudia Goupil Alexis Duringer Andréanne Loiselle Eva Bouchard Daniel Agudelo Yannick Doyon |
| author_facet | Sébastien Levesque Diana Mayorga Jean-Philippe Fiset Claudia Goupil Alexis Duringer Andréanne Loiselle Eva Bouchard Daniel Agudelo Yannick Doyon |
| author_sort | Sébastien Levesque |
| collection | DOAJ |
| description | Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. Here the authors develop a co-selection strategy to facilitate prime editing in human cells and provide design principles to prevent the formation of undesired editing byproducts at the target site. |
| first_indexed | 2024-04-12T09:29:44Z |
| format | Article |
| id | doaj.art-edf7cdca85ce4089b7c630fd93fd7034 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T09:29:44Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-edf7cdca85ce4089b7c630fd93fd70342022-12-22T03:38:24ZengNature PortfolioNature Communications2041-17232022-10-0113111410.1038/s41467-022-33669-zMarker-free co-selection for successive rounds of prime editing in human cellsSébastien Levesque0Diana Mayorga1Jean-Philippe Fiset2Claudia Goupil3Alexis Duringer4Andréanne Loiselle5Eva Bouchard6Daniel Agudelo7Yannick Doyon8Centre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalCentre Hospitalier Universitaire de Québec Research Center—Université LavalPrime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. Here the authors develop a co-selection strategy to facilitate prime editing in human cells and provide design principles to prevent the formation of undesired editing byproducts at the target site.https://doi.org/10.1038/s41467-022-33669-z |
| spellingShingle | Sébastien Levesque Diana Mayorga Jean-Philippe Fiset Claudia Goupil Alexis Duringer Andréanne Loiselle Eva Bouchard Daniel Agudelo Yannick Doyon Marker-free co-selection for successive rounds of prime editing in human cells Nature Communications |
| title | Marker-free co-selection for successive rounds of prime editing in human cells |
| title_full | Marker-free co-selection for successive rounds of prime editing in human cells |
| title_fullStr | Marker-free co-selection for successive rounds of prime editing in human cells |
| title_full_unstemmed | Marker-free co-selection for successive rounds of prime editing in human cells |
| title_short | Marker-free co-selection for successive rounds of prime editing in human cells |
| title_sort | marker free co selection for successive rounds of prime editing in human cells |
| url | https://doi.org/10.1038/s41467-022-33669-z |
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