A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1987-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520386545 |
| _version_ | 1818647464086863872 |
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| author | J C Khoo E Miller D I Goldberg |
| author_facet | J C Khoo E Miller D I Goldberg |
| author_sort | J C Khoo |
| collection | DOAJ |
| description | An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles. |
| first_indexed | 2024-12-17T01:02:57Z |
| format | Article |
| id | doaj.art-edffbec23a754e428c00e1386b601ab2 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T01:02:57Z |
| publishDate | 1987-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-edffbec23a754e428c00e1386b601ab22022-12-21T22:09:23ZengElsevierJournal of Lipid Research0022-22751987-07-01287873877A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.J C KhooE MillerD I GoldbergAn enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.http://www.sciencedirect.com/science/article/pii/S0022227520386545 |
| spellingShingle | J C Khoo E Miller D I Goldberg A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. Journal of Lipid Research |
| title | A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. |
| title_full | A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. |
| title_fullStr | A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. |
| title_full_unstemmed | A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. |
| title_short | A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides. |
| title_sort | rapid and sensitive enzymatic radiochemical assay for the determination of triglycerides |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520386545 |
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