Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period
Non-buffered plasma-activated liquids such as water and saline have shown bactericidal effects. In the present study, we investigated the anti-bacterial efficacy and stability of plasma-activated water (PAW) and plasma-activated saline (PAS), generated using a high voltage dielectric barrier dischar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/12/11/3021 |
| _version_ | 1797549633769046016 |
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| author | Evanthia Tsoukou Paula Bourke Daniela Boehm |
| author_facet | Evanthia Tsoukou Paula Bourke Daniela Boehm |
| author_sort | Evanthia Tsoukou |
| collection | DOAJ |
| description | Non-buffered plasma-activated liquids such as water and saline have shown bactericidal effects. In the present study, we investigated the anti-bacterial efficacy and stability of plasma-activated water (PAW) and plasma-activated saline (PAS), generated using a high voltage dielectric barrier discharge system. This study compares the potential of non-buffered plasma-activated liquids (PAL) for the inactivation of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>) after storage of the solutions at five different temperatures for a storage time up to 18 months after their generation. The temperatures used were room temperature, 4 °C, −16 °C, −80 °C, −150 °C. Both PAW and PAS achieved 6 log reduction for both bacteria on the first day of their generation after 60 min contact time and they retained these effects after 18 months when stored at the lowest temperatures. Chemical analysis of PAL showed that plasma caused a drop in pH, generation of reactive oxygen species and nitrates, whereas no nitrites are detected in the system used. The concentrations of chemical species were affected by the storage at different temperatures and a thermocouple probe was used to investigate the freezing behaviour of the PAL. |
| first_indexed | 2024-03-10T15:17:21Z |
| format | Article |
| id | doaj.art-ee0265a162494e7291d615d54a0efc41 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T15:17:21Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-ee0265a162494e7291d615d54a0efc412023-11-20T18:49:46ZengMDPI AGWater2073-44412020-10-011211302110.3390/w12113021Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months PeriodEvanthia Tsoukou0Paula Bourke1Daniela Boehm2Environmental Sustainability and Health Institute and School of Food Science and Environmental Health, Technological University Dublin, Dublin, IrelandEnvironmental Sustainability and Health Institute and School of Food Science and Environmental Health, Technological University Dublin, Dublin, IrelandEnvironmental Sustainability and Health Institute and School of Food Science and Environmental Health, Technological University Dublin, Dublin, IrelandNon-buffered plasma-activated liquids such as water and saline have shown bactericidal effects. In the present study, we investigated the anti-bacterial efficacy and stability of plasma-activated water (PAW) and plasma-activated saline (PAS), generated using a high voltage dielectric barrier discharge system. This study compares the potential of non-buffered plasma-activated liquids (PAL) for the inactivation of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>) after storage of the solutions at five different temperatures for a storage time up to 18 months after their generation. The temperatures used were room temperature, 4 °C, −16 °C, −80 °C, −150 °C. Both PAW and PAS achieved 6 log reduction for both bacteria on the first day of their generation after 60 min contact time and they retained these effects after 18 months when stored at the lowest temperatures. Chemical analysis of PAL showed that plasma caused a drop in pH, generation of reactive oxygen species and nitrates, whereas no nitrites are detected in the system used. The concentrations of chemical species were affected by the storage at different temperatures and a thermocouple probe was used to investigate the freezing behaviour of the PAL.https://www.mdpi.com/2073-4441/12/11/3021plasma activated liquids (PAL)plasma activated water (PAW)plasma activated saline (PAS)storagetemperature stabilityROS |
| spellingShingle | Evanthia Tsoukou Paula Bourke Daniela Boehm Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period Water plasma activated liquids (PAL) plasma activated water (PAW) plasma activated saline (PAS) storage temperature stability ROS |
| title | Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period |
| title_full | Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period |
| title_fullStr | Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period |
| title_full_unstemmed | Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period |
| title_short | Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period |
| title_sort | temperature stability and effectiveness of plasma activated liquids over an 18 months period |
| topic | plasma activated liquids (PAL) plasma activated water (PAW) plasma activated saline (PAS) storage temperature stability ROS |
| url | https://www.mdpi.com/2073-4441/12/11/3021 |
| work_keys_str_mv | AT evanthiatsoukou temperaturestabilityandeffectivenessofplasmaactivatedliquidsoveran18monthsperiod AT paulabourke temperaturestabilityandeffectivenessofplasmaactivatedliquidsoveran18monthsperiod AT danielaboehm temperaturestabilityandeffectivenessofplasmaactivatedliquidsoveran18monthsperiod |