Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin
Alloxan and streptozotocin are the most popular diabetogenic agents in assessing antidiabetic activity. Self-recovery, indicated by unstable hyperglycemia conditions in animals induced by those agents, becomes a significant disturbance to accurate examination. This study aimed to evaluate and reveal...
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Elsevier
2023-05-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023027408 |
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| author | Indah Fajarwati Dedy Duryadi Solihin Tutik Wresdiyati Irmanida Batubara |
| author_facet | Indah Fajarwati Dedy Duryadi Solihin Tutik Wresdiyati Irmanida Batubara |
| author_sort | Indah Fajarwati |
| collection | DOAJ |
| description | Alloxan and streptozotocin are the most popular diabetogenic agents in assessing antidiabetic activity. Self-recovery, indicated by unstable hyperglycemia conditions in animals induced by those agents, becomes a significant disturbance to accurate examination. This study aimed to evaluate and reveal the self-recovery incidence in Sprague Dawley rats induced with alloxan and streptozotocin. Each dose of alloxan (120, 150, 180 mg/kg) and streptozotocin (40, 50, 60 mg/kg) was administered through intraperitoneal injection. The results showed that each dose of alloxan induced self-recovery incidence. In rats given streptozotocin, self-recovery only occurred at a dose of 40 mg/kg. The other higher doses of streptozotocin induced stable hyperglycemia. Furthermore, this study revealed two types of self-recovery, namely temporary recovery and end recovery. Temporary recovery occurred in rats given alloxan, during end recovery in alloxan and streptozotocin. The examination of insulin levels showed a significant reduction in the temporary recovery and stable diabetic rats compared to the end recovery rats. Besides, the bodyweight of rats was also affected by different incidences of self-recovery. This study recommends paying more attention to the possibility of self-recovery in obtaining animal models of diabetes, emphasizing the determination of suitable diabetogenic agents and proper doses to reduce self-recovery incidences. The finding of temporary recovery in rats receiving alloxan indicates that alloxan induced delayed diabetes in rats. |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:27:21Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ee042cfd08b145b183999aaecffb6d242023-05-31T04:44:42ZengElsevierHeliyon2405-84402023-05-0195e15533Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocinIndah Fajarwati0Dedy Duryadi Solihin1Tutik Wresdiyati2Irmanida Batubara3Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia; Corresponding author.Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia; Corresponding author.Divisions of Anatomy, Histology, and Embriology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Jalan Agatis Kampus IPB Dramaga, Bogor 16680, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia; Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jalan Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, IndonesiaAlloxan and streptozotocin are the most popular diabetogenic agents in assessing antidiabetic activity. Self-recovery, indicated by unstable hyperglycemia conditions in animals induced by those agents, becomes a significant disturbance to accurate examination. This study aimed to evaluate and reveal the self-recovery incidence in Sprague Dawley rats induced with alloxan and streptozotocin. Each dose of alloxan (120, 150, 180 mg/kg) and streptozotocin (40, 50, 60 mg/kg) was administered through intraperitoneal injection. The results showed that each dose of alloxan induced self-recovery incidence. In rats given streptozotocin, self-recovery only occurred at a dose of 40 mg/kg. The other higher doses of streptozotocin induced stable hyperglycemia. Furthermore, this study revealed two types of self-recovery, namely temporary recovery and end recovery. Temporary recovery occurred in rats given alloxan, during end recovery in alloxan and streptozotocin. The examination of insulin levels showed a significant reduction in the temporary recovery and stable diabetic rats compared to the end recovery rats. Besides, the bodyweight of rats was also affected by different incidences of self-recovery. This study recommends paying more attention to the possibility of self-recovery in obtaining animal models of diabetes, emphasizing the determination of suitable diabetogenic agents and proper doses to reduce self-recovery incidences. The finding of temporary recovery in rats receiving alloxan indicates that alloxan induced delayed diabetes in rats.http://www.sciencedirect.com/science/article/pii/S2405844023027408Alloxan-induced diabetesDiabetogenic agentsIntraperitoneal routeRecoverySprague Dawley ratsStable diabetes |
| spellingShingle | Indah Fajarwati Dedy Duryadi Solihin Tutik Wresdiyati Irmanida Batubara Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin Heliyon Alloxan-induced diabetes Diabetogenic agents Intraperitoneal route Recovery Sprague Dawley rats Stable diabetes |
| title | Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin |
| title_full | Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin |
| title_fullStr | Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin |
| title_full_unstemmed | Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin |
| title_short | Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin |
| title_sort | self recovery in diabetic sprague dawley rats induced by intraperitoneal alloxan and streptozotocin |
| topic | Alloxan-induced diabetes Diabetogenic agents Intraperitoneal route Recovery Sprague Dawley rats Stable diabetes |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023027408 |
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