Production of specific polyclonal antibodies anti-human factor IX
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to an...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2006-07-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010 |
| _version_ | 1818561534703435776 |
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| author | Daniel Gonçalves Chaves Cibele Velloso Rodrigues Wanderley Almeida Ferreira Pollyana Fantini Miranda Siqueira Clara Guerra Duarte Anna Bárbara de Freitas Carneiro-Proietti Marcelo Matos Santoro |
| author_facet | Daniel Gonçalves Chaves Cibele Velloso Rodrigues Wanderley Almeida Ferreira Pollyana Fantini Miranda Siqueira Clara Guerra Duarte Anna Bárbara de Freitas Carneiro-Proietti Marcelo Matos Santoro |
| author_sort | Daniel Gonçalves Chaves |
| collection | DOAJ |
| description | In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.<br>Neste trabalho foram produzidos anticorpos policlonais anti-fator IX humano em coelhos New Zealand imunizados com FIX humano (hFIX) comercial puro (Octanyne®, Octapharma, EUA). O soro contendo as imunoglobulinas anti-hFIX foi útil para a detecção do antígeno hFIX em frações do plasma humano submetido a cromatografia de troca iônica. Imunoensaios (ELISA) usando soro-albumina bovina, tripsina e peptídeos gerados por ensaios de clivagem com tripsina com enzima de digestão foram realizados e revelaram especificidade adequada dos anticorpos policlonais produzidos. |
| first_indexed | 2024-12-14T00:52:06Z |
| format | Article |
| id | doaj.art-ee04409a665c432ebf041b37ec1cc305 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-14T00:52:06Z |
| publishDate | 2006-07-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ee04409a665c432ebf041b37ec1cc3052022-12-21T23:23:47ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242006-07-0149460560910.1590/S1516-89132006000500010Production of specific polyclonal antibodies anti-human factor IXDaniel Gonçalves ChavesCibele Velloso RodriguesWanderley Almeida FerreiraPollyana Fantini Miranda SiqueiraClara Guerra DuarteAnna Bárbara de Freitas Carneiro-ProiettiMarcelo Matos SantoroIn this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.<br>Neste trabalho foram produzidos anticorpos policlonais anti-fator IX humano em coelhos New Zealand imunizados com FIX humano (hFIX) comercial puro (Octanyne®, Octapharma, EUA). O soro contendo as imunoglobulinas anti-hFIX foi útil para a detecção do antígeno hFIX em frações do plasma humano submetido a cromatografia de troca iônica. Imunoensaios (ELISA) usando soro-albumina bovina, tripsina e peptídeos gerados por ensaios de clivagem com tripsina com enzima de digestão foram realizados e revelaram especificidade adequada dos anticorpos policlonais produzidos.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010Factor IXhemophilia BHPLCpurificationantibodiesELISA |
| spellingShingle | Daniel Gonçalves Chaves Cibele Velloso Rodrigues Wanderley Almeida Ferreira Pollyana Fantini Miranda Siqueira Clara Guerra Duarte Anna Bárbara de Freitas Carneiro-Proietti Marcelo Matos Santoro Production of specific polyclonal antibodies anti-human factor IX Brazilian Archives of Biology and Technology Factor IX hemophilia B HPLC purification antibodies ELISA |
| title | Production of specific polyclonal antibodies anti-human factor IX |
| title_full | Production of specific polyclonal antibodies anti-human factor IX |
| title_fullStr | Production of specific polyclonal antibodies anti-human factor IX |
| title_full_unstemmed | Production of specific polyclonal antibodies anti-human factor IX |
| title_short | Production of specific polyclonal antibodies anti-human factor IX |
| title_sort | production of specific polyclonal antibodies anti human factor ix |
| topic | Factor IX hemophilia B HPLC purification antibodies ELISA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010 |
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