Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana
<p>Abstract</p> <p>Background</p> <p>Mosquito resistance to the pyrethroid insecticides used to treat bednets threatens the sustainability of malaria control in sub-Saharan Africa. While the impact of target site insensitivity alleles is being widely discussed the impli...
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| Format: | Article |
| Language: | English |
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BMC
2007-01-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/36 |
| _version_ | 1818675694607007744 |
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| author | Donnelly Martin J Müller Pie Ranson Hilary |
| author_facet | Donnelly Martin J Müller Pie Ranson Hilary |
| author_sort | Donnelly Martin J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Mosquito resistance to the pyrethroid insecticides used to treat bednets threatens the sustainability of malaria control in sub-Saharan Africa. While the impact of target site insensitivity alleles is being widely discussed the implications of insecticide detoxification – though equally important – remains elusive. The successful development of new tools for malaria intervention and management requires a comprehensive understanding of insecticide resistance, including metabolic resistance mechanisms. Although three enzyme families (cytochrome P450s, glutathione S-transferases and carboxylesterases) have been widely associated with insecticide detoxification the role of individual enzymes is largely unknown.</p> <p>Results</p> <p>Here, constitutive expression patterns of genes putatively involved in conferring pyrethroid resistance was investigated in a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Odumasy, Southern Ghana. RNA from the resistant strain and a standard laboratory susceptible strain, of both sexes was extracted, reverse transcribed and labelled with either Cy3- or Cy5-dye. Labelled cDNA was co-hybridised to the <it>detox chip</it>, a custom-made microarray containing over 230 <it>A. gambiae </it>gene fragments predominantly from enzyme families associated with insecticide resistance. After hybridisation, Cy3- and Cy5-signal intensities were measured and compared gene by gene. In both females and males of the resistant strain the cytochrome P450s <it>CYP6Z2 </it>and <it>CYP6M2 </it>are highly over-expressed along with a member of the <it>superoxide dismutase </it>(<it>SOD</it>) gene family.</p> <p>Conclusion</p> <p>These genes differ from those found up-regulated in East African strains of pyrethroid resistant <it>A. gambiae </it>and constitute a novel set of candidate genes implicated in insecticide detoxification. These data suggest that metabolic resistance may have multiple origins in <it>A. gambiae</it>, which has strong implications for the management of resistance.</p> |
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| format | Article |
| id | doaj.art-ee057cb493fe4c30af86fefc537a2f16 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T08:31:39Z |
| publishDate | 2007-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-ee057cb493fe4c30af86fefc537a2f162022-12-21T21:56:35ZengBMCBMC Genomics1471-21642007-01-01813610.1186/1471-2164-8-36Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from GhanaDonnelly Martin JMüller PieRanson Hilary<p>Abstract</p> <p>Background</p> <p>Mosquito resistance to the pyrethroid insecticides used to treat bednets threatens the sustainability of malaria control in sub-Saharan Africa. While the impact of target site insensitivity alleles is being widely discussed the implications of insecticide detoxification – though equally important – remains elusive. The successful development of new tools for malaria intervention and management requires a comprehensive understanding of insecticide resistance, including metabolic resistance mechanisms. Although three enzyme families (cytochrome P450s, glutathione S-transferases and carboxylesterases) have been widely associated with insecticide detoxification the role of individual enzymes is largely unknown.</p> <p>Results</p> <p>Here, constitutive expression patterns of genes putatively involved in conferring pyrethroid resistance was investigated in a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Odumasy, Southern Ghana. RNA from the resistant strain and a standard laboratory susceptible strain, of both sexes was extracted, reverse transcribed and labelled with either Cy3- or Cy5-dye. Labelled cDNA was co-hybridised to the <it>detox chip</it>, a custom-made microarray containing over 230 <it>A. gambiae </it>gene fragments predominantly from enzyme families associated with insecticide resistance. After hybridisation, Cy3- and Cy5-signal intensities were measured and compared gene by gene. In both females and males of the resistant strain the cytochrome P450s <it>CYP6Z2 </it>and <it>CYP6M2 </it>are highly over-expressed along with a member of the <it>superoxide dismutase </it>(<it>SOD</it>) gene family.</p> <p>Conclusion</p> <p>These genes differ from those found up-regulated in East African strains of pyrethroid resistant <it>A. gambiae </it>and constitute a novel set of candidate genes implicated in insecticide detoxification. These data suggest that metabolic resistance may have multiple origins in <it>A. gambiae</it>, which has strong implications for the management of resistance.</p>http://www.biomedcentral.com/1471-2164/8/36 |
| spellingShingle | Donnelly Martin J Müller Pie Ranson Hilary Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana BMC Genomics |
| title | Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana |
| title_full | Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana |
| title_fullStr | Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana |
| title_full_unstemmed | Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana |
| title_short | Transcription profiling of a recently colonised pyrethroid resistant <it>Anopheles gambiae </it>strain from Ghana |
| title_sort | transcription profiling of a recently colonised pyrethroid resistant it anopheles gambiae it strain from ghana |
| url | http://www.biomedcentral.com/1471-2164/8/36 |
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